Recombinant Human UbcH5a/UBE2D1 Protein, CF | R&D Systems E2-616 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human UbcH5a/UBE2D1 Protein, CF

catalog :

E2-616

quantity :

100 ug

price :

239 USD

citations: 31

Reference
Li X, Guo M, Cai L, Du T, Liu Y, Ding H, et al. Competitive ubiquitination activates the tumor suppressor p53. Cell Death Differ. 2020;27:1807-1818 pubmed publisher
Ting T, Goralski M, Klein K, Wang B, Kim J, Xie Y, et al. Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15. Cell Rep. 2019;29:1499-1510.e6 pubmed publisher
Sitz J, Blanchet S, Gameiro S, Biquand E, Morgan T, Galloy M, et al. Human papillomavirus E7 oncoprotein targets RNF168 to hijack the host DNA damage response. Proc Natl Acad Sci U S A. 2019;116:19552-19562 pubmed publisher
Yip M, Keszei A, Feng Q, Chu V, McKenna M, Shao S. Mechanism for recycling tRNAs on stalled ribosomes. Nat Struct Mol Biol. 2019;26:343-349 pubmed publisher
Lu G, Yi J, Gubas A, Wang Y, Wu Y, Ren Y, et al. Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation. Autophagy. 2019;:1-18 pubmed publisher
Akande O, Damle P, Pop M, Sherman N, Szomju B, Litovchick L, et al. DBC1 Regulates p53 Stability via Inhibition of CBP-Dependent p53 Polyubiquitination. Cell Rep. 2019;26:3323-3335.e4 pubmed publisher
Dong Y, Zhang S, Wu Z, Li X, Wang W, Zhu Y, et al. Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome. Nature. 2019;565:49-55 pubmed publisher
Matyskiela M, Couto S, Zheng X, Lu G, Hui J, Stamp K, et al. SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrate. Nat Chem Biol. 2018;14:981-987 pubmed publisher
Amin P, Florez M, Najafov A, Pan H, Geng J, Ofengeim D, et al. Regulation of a distinct activated RIPK1 intermediate bridging complex I and complex II in TNFα-mediated apoptosis. Proc Natl Acad Sci U S A. 2018;115:E5944-E5953 pubmed publisher
Ito S, Ueno A, Ueda T, Nakagawa H, Taniguchi H, Kayukawa N, et al. CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells. Oncotarget. 2018;9:17645-17655 pubmed publisher
Khanna R, Krishnamoorthy V, Parnaik V. E3 ubiquitin ligase RNF123 targets lamin B1 and lamin-binding proteins. FEBS J. 2018;285:2243-2262 pubmed publisher
Ratliff M, Hill Harfe K, Gleason E, Ling H, Kroft T, L hernault S. MIB-1 Is Required for Spermatogenesis and Facilitates LIN-12 and GLP-1 Activity in Caenorhabditis elegans. Genetics. 2018;209:173-193 pubmed publisher
Zheng L, Conner S. PI5P4K? functions in DTX1-mediated Notch signaling. Proc Natl Acad Sci U S A. 2018;115:E1983-E1990 pubmed publisher
Murali A, Shin J, Yurugi H, Krishnan A, Akutsu M, Carpy A, et al. Ubiquitin-dependent regulation of Cdc42 by XIAP. Cell Death Dis. 2017;8:e2900 pubmed publisher
Glorian V, Allègre J, Berthelet J, Dumetier B, Boutanquoi P, Droin N, et al. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues. Cell Death Dis. 2017;8:e2816 pubmed publisher
An J, Ponthier C, Sack R, Seebacher J, Stadler M, Donovan K, et al. pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4CRBN ubiquitin ligase. Nat Commun. 2017;8:15398 pubmed publisher
Yang C, Jividen K, Spencer A, Dworak N, Ni L, Oostdyk L, et al. Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9. Mol Cell. 2017;66:503-516.e5 pubmed publisher
Lee Y, Chou T, Pittman S, Keith A, Razani B, Weihl C. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination. Cell Rep. 2017;19:188-202 pubmed publisher
Kliza K, Taumer C, Pinzuti I, Franz Wachtel M, Kunzelmann S, Stieglitz B, et al. Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry. Nat Methods. 2017;14:504-512 pubmed publisher
Nakamura T, Murakami K, Tada H, Uehara Y, Nogami J, Maehara K, et al. Thymine DNA glycosylase modulates DNA damage response and gene expression by base excision repair-dependent and independent mechanisms. Genes Cells. 2017;22:392-405 pubmed publisher
McClurg U, Cork D, Darby S, Ryan Munden C, Nakjang S, Mendes Côrtes L, et al. Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity. Nucleic Acids Res. 2017;45:1793-1804 pubmed publisher
Du L, LI Y, Fakih M, Wiatrek R, Duldulao M, Chen Z, et al. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal. Nat Commun. 2016;7:12326 pubmed publisher
Matyskiela M, Lu G, Ito T, Pagarigan B, Lu C, Miller K, et al. A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase. Nature. 2016;535:252-7 pubmed publisher
Sohn S, Hearing P. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation. Proc Natl Acad Sci U S A. 2016;113:6725-30 pubmed publisher
Sanchez C, Liu C, Mayer S, Nurhasanah A, Cyrklaff M, Mu J, et al. A HECT ubiquitin-protein ligase as a novel candidate gene for altered quinine and quinidine responses in Plasmodium falciparum. PLoS Genet. 2014;10:e1004382 pubmed publisher
Guicciardi M, Werneburg N, Bronk S, Franke A, Yagita H, Thomas G, et al. Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity. PLoS ONE. 2014;9:e92124 pubmed publisher
Townley Tilson W, Wu Y, Ferguson J, Patterson C. The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2. PLoS ONE. 2014;9:e89451 pubmed publisher
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product information

brand :

R&D Systems, powered by Boston Biochem

master code :

E2-616

SKU :

E2-616-100

product name :

Recombinant Human UbcH5a/UBE2D1 Protein, CF

description :

The Recombinant Human UbcH5a/UBE2D1 Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UbcH5a/UBE2D1 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

UbcH5a / UBE2D1

category :

Proteins and Enzymes

unit size :

100 ug

buffer :

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP

conjugate :

Unconjugated

main entrez gene id :

7321

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

species :

Human

theoretical molecular weight :

17 kDa

gene symbol :

UBE2D1

details of functionality :

Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5a/UBE2D1 concentration of 0.1-1 µM.

catalog number base :

E2-616

accessionNumbers :

P51668

applications :

Enzyme Activity

source :

The Recombinant Human UbcH5a / UBE2D1 Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UbcH5a / UBE2D1 Protein, CF has been validated for the following applications: Enzyme Activity.

USD :

239 USD

alt names :

E2(17)KB1, Stimulator of Fe transportUBC4/5 homolog, UBC4/5, UBC5A, UbcH5, UbcH5A, UBCH5AEC 6.3.2.19, UBCH5SFT, UBE2D1, Ubiquitin carrier protein D1, ubiquitin-conjugating enzyme E2 D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast), Ubiquitin-protein ligase D1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.