Recombinant Human His8-USP7 Protein, CF | R&D Systems E-519-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human His8-USP7 Protein, CF

catalog :

E-519-025

quantity :

25 ug

price :

400 USD

more info or order :

R&D Systems product webpage

citations: 7

Reference
Sun Y, Chen J, Huang S, Su Y, Wang W, Agama K, et al. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation. Nat Commun. 2021;12:5010 pubmed publisher
Cathcart A, Bird G, Wales T, Herce H, Harvey E, Hauseman Z, et al. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer. Nat Chem Biol. 2020;16:1218-1226 pubmed publisher
Kim J, Yang Y, Park K, Ge X, Xu R, Li N, et al. A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation. Nat Commun. 2020;11:2289 pubmed publisher
Gavory G, O Dowd C, Helm M, Flasz J, Arkoudis E, Dossang A, et al. Discovery and characterization of highly potent and selective allosteric USP7 inhibitors. Nat Chem Biol. 2017;: pubmed publisher
LandrÃ© V, Revi B, Mir M, Verma C, Hupp T, Gilbert N, et al. Regulation of transcriptional activators by DNA-binding domain ubiquitination. Cell Death Differ. 2017;24:903-916 pubmed publisher
Papp S, Huber A, Jordan S, Kriebs A, Nguyen M, Moresco J, et al. DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization. elife. 2015;4: pubmed publisher
Liang Q, Dexheimer T, Zhang P, Rosenthal A, Villamil M, You C, et al. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nat Chem Biol. 2014;10:298-304 pubmed publisher

product information

master code :

E-519

SKU :

E-519-025

product name :

Recombinant Human His8-USP7 Protein, CF

unit size :

25 ug

description :

The Recombinant Human His8-USP7 Protein, CF from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human His8-USP7 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

USP7

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in HEPES, NaCl, EDTA, DTT, and Glycerol.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.

species :

Human

theoretical molecular weight :

130 kDa

gene symbol :

USP7

details of functionality :

Recombinant Human His8-USP7 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His8-USP7 concentration of 1-5 nM.

accessionNumbers :

Q93009.2

applications :

Enzyme Activity

source long :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived human USP7 protein Met1 - Asn1102 with a N-terminal 8-His tag

source short :

Sf 21 (baculovirus)

USD :

400 USD

alt names :

Deubiquitinating enzyme 7, EC 3.1.2.15, EC 3.4.19.12, HAUSP, HAUSPTEF1, Herpes virus-associated ubiquitin-specific protease, Herpesvirus-associated ubiquitin-specific protease, TEF1, ubiquitin carboxyl-terminal hydrolase 7, ubiquitin specific peptidase 7 (herpes virus-associated), ubiquitin specific protease 7 (herpes virus-associated), Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage