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company name :
R&D Systems
product type :
protein
product name :
Human 20S Proteasome Protein, CF
catalog :
E-360-050
quantity :
50 ug
price :
294 USD
more info or order :
citations: 28
Reference
Davidson K, Bano M, Parker D, Osmulski P, Gaczynska M, Pickering A. β-Amyloid impairs Proteasome structure and function. Proteasome activation mitigates amyloid induced toxicity and cognitive deficits. bioRxiv. 2025;: pubmed publisher
Zimbone S, Romanucci V, Zarrelli A, Giuffrida M, Sciacca M, Lanza V, et al. Exploring the therapeutic potential of Aloin: unraveling neuroprotective and anticancer mechanisms, and strategies for enhanced stability and delivery. Sci Rep. 2024;14:16731 pubmed publisher
Wellington D, Yin Z, Yu Z, Heilig R, Davis S, Fischer R, et al. SARS-CoV-2 mutations affect antigen processing by the proteasome to alter CD8+ T cell responses. Heliyon. 2023;9:e20076 pubmed publisher
Tundo G, Sbardella D, Oddone F, Grasso G, Marini S, Atzori M, et al. Insulin-Degrading Enzyme Is a Non Proteasomal Target of Carfilzomib and Affects the 20S Proteasome Inhibition by the Drug. Biomolecules. 2022;12: pubmed publisher
Lee J, Pandey A, Venkatesh S, Thilagavathi J, Honda T, Singh K, et al. Inhibition of mitochondrial LonP1 protease by allosteric blockade of ATP binding and hydrolysis via CDDO and its derivatives. J Biol Chem. 2022;298:101719 pubmed publisher
Wang B, Zhu Y, Yu C, Zhang C, Tang Q, Huang H, et al. Hepatitis C virus induces oxidation and degradation of apolipoprotein B to enhance lipid accumulation and promote viral production. PLoS Pathog. 2021;17:e1009889 pubmed publisher
Guan H, Wang Y, Yu T, Huang Y, Li M, Saeed A, et al. Cryo-EM structures of the human PA200 and PA200-20S complex reveal regulation of proteasome gate opening and two PA200 apertures. PLoS Biol. 2020;18:e3000654 pubmed publisher
Yeo I, Lee M, Baek A, Miller Z, Bhattarai D, Baek Y, et al. A dual inhibitor of the proteasome catalytic subunits LMP2 and Y attenuates disease progression in mouse models of Alzheimer's disease. Sci Rep. 2019;9:18393 pubmed publisher
Scarpino A, Bajusz D, Proj M, Gobec M, Sosič I, Gobec S, et al. Discovery of Immunoproteasome Inhibitors Using Large-Scale Covalent Virtual Screening. Molecules. 2019;24: pubmed publisher
Li X, Huang Q, Long H, Zhang P, Su H, Liu J. A new gold(I) complex-Au(PPh3)PT is a deubiquitinase inhibitor and inhibits tumor growth. EBioMedicine. 2019;39:159-172 pubmed publisher
Wang Q, Gao X, Yu T, Yuan L, Dai J, Wang W, et al. REGγ Controls Hippo Signaling and Reciprocal NF-κB-YAP Regulation to Promote Colon Cancer. Clin Cancer Res. 2018;24:2015-2025 pubmed publisher
Bosc E, Nastri J, Lefort V, Valli M, Contiguiba F, Pioli R, et al. Piperlongumine and some of its analogs inhibit selectively the human immunoproteasome over the constitutive proteasome. Biochem Biophys Res Commun. 2018;496:961-966 pubmed publisher
Winter M, La Greca F, Arastu Kapur S, Caiazza F, Cimermancic P, Buchholz T, et al. Immunoproteasome functions explained by divergence in cleavage specificity and regulation. elife. 2017;6: pubmed publisher
Mikkonen E, Haglund C, Holmberg C. Immunohistochemical analysis reveals variations in proteasome tissue expression in C. elegans. PLoS ONE. 2017;12:e0183403 pubmed publisher
Chang A, Dao T, Gejman R, Jarvis C, Scott A, Dubrovsky L, et al. A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens. J Clin Invest. 2017;127:2705-2718 pubmed publisher
Zhou W, Wei L, Xiao T, Lai C, Peng M, Xu L, et al. Diabetogenic agent alloxan is a proteasome inhibitor. Biochem Biophys Res Commun. 2017;488:400-406 pubmed publisher
Palumbo R, Gogliettino M, Cocca E, Iannitti R, Sandomenico A, Ruvo M, et al. APEH Inhibition Affects Osteosarcoma Cell Viability via Downregulation of the Proteasome. Int J Mol Sci. 2016;17: pubmed publisher
Santoro A, Monaco I, Attanasio F, Lanza V, Pappalardo G, Tomasello M, et al. Copper(II) ions affect the gating dynamics of the 20S proteasome: a molecular and in cell study. Sci Rep. 2016;6:33444 pubmed publisher
Lee S, Levitsky K, Parlati F, Bennett M, Arastu Kapur S, Kellerman L, et al. Clinical activity of carfilzomib correlates with inhibition of multiple proteasome subunits: application of a novel pharmacodynamic assay. Br J Haematol. 2016;173:884-95 pubmed publisher
Li H, O Donoghue A, van der Linden W, Xie S, Yoo E, Foe I, et al. Structure- and function-based design of Plasmodium-selective proteasome inhibitors. Nature. 2016;530:233-6 pubmed publisher
Pundir S, Vu H, Solomon V, McClure R, Lee H. VR23: A Quinoline-Sulfonyl Hybrid Proteasome Inhibitor That Selectively Kills Cancer via Cyclin E-Mediated Centrosome Amplification. Cancer Res. 2015;75:4164-75 pubmed publisher
Akopian T, Kandror O, Tsu C, Lai J, Wu W, Liu Y, et al. Cleavage Specificity of Mycobacterium tuberculosis ClpP1P2 Protease and Identification of Novel Peptide Substrates and Boronate Inhibitors with Anti-bacterial Activity. J Biol Chem. 2015;290:11008-20 pubmed publisher
Maehama T, Kawahara K, Nishio M, Suzuki A, Hanada K. Nucleolar stress induces ubiquitination-independent proteasomal degradation of PICT1 protein. J Biol Chem. 2014;289:20802-12 pubmed
Debowski D, Pikuła M, Lubos M, Langa P, Trzonkowski P, Lesner A, et al. Inhibition of human and yeast 20S proteasome by analogues of trypsin inhibitor SFTI-1. PLoS ONE. 2014;9:e89465 pubmed publisher
Nardon C, Schmitt S, Yang H, Zuo J, Fregona D, Dou Q. Gold(III)-dithiocarbamato peptidomimetics in the forefront of the targeted anticancer therapy: preclinical studies against human breast neoplasia. PLoS ONE. 2014;9:e84248 pubmed publisher
Bergamo P, Cocca E, Palumbo R, Gogliettino M, Rossi M, Palmieri G. RedOx status, proteasome and APEH: insights into anticancer mechanisms of t10,c12-conjugated linoleic acid isomer on A375 melanoma cells. PLoS ONE. 2013;8:e80900 pubmed publisher
Henle A, Erskine C, Benson L, Clynes R, Knutson K. Enzymatic discovery of a HER-2/neu epitope that generates cross-reactive T cells. J Immunol. 2013;190:479-88 pubmed publisher
Gobec M, Obreza A, Prijatelj M, Brus B, Gobec S, Mlinaric Rascan I. Selective cytotoxicity of amidinopiperidine based compounds towards Burkitt's lymphoma cells involves proteasome inhibition. PLoS ONE. 2012;7:e41961 pubmed publisher
product information
master code :
E-360
SKU :
E-360-050
product name :
Human 20S Proteasome Protein, CF
unit size :
50 ug
description :
The Human 20S Proteasome Protein, CF from R&D Systems is derived from Human Erythrocytes. The Human 20S Proteasome Protein, CF has been validated for the following applications: Enzyme Activity.
target :
20S Proteasome
category :
Proteins and Enzymes
buffer :
Supplied as a 0.2 ╡m filtered solution in HEPES, NaCl and DTT.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
theoretical molecular weight :
700 kDa
details of functionality :
The Human 20S Proteasome is able to degrade substrates in an ATP-independent manner. Reaction conditions will need to be optimized for each specific application. We recommend an initial Human 20S Proteasome concentration of 0.5-5 nM.
applications :
Enzyme Activity
source long :
Human erythrocyte-derived 20S Proteasome protein The Human 20S Proteasome protein has been purified from human erythrocytes, which have been screened and are negative for hepatitis B surface antigen, antibodies to hepatitis C virus, HIV type 1 antigens, and antibodies to HIV type 1 and 2.
source short :
Human Erythrocytes
USD :
294 USD
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.