Recombinant Human UCH-L1/PGP9.5 Protein, CF | R&D Systems E-340 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human UCH-L1/PGP9.5 Protein, CF

catalog :

E-340

quantity :

25 ug

price :

125 USD

citations: 5

Reference
Hewings D, Heideker J, Ma T, AhYoung A, El Oualid F, Amore A, et al. Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes. Nat Commun. 2018;9:1162 pubmed publisher
Toliczenko Bernatowicz D, Matuszczak E, Tylicka M, Szymanska B, Komarowska M, Gorodkiewicz E, et al. Overexpression of ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) in boys with cryptorchidism. PLoS ONE. 2018;13:e0191806 pubmed publisher
Matuszczak E, Tylicka M, Debek W, Sankiewicz A, Gorodkiewicz E, Hermanowicz A. Overexpression of ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) in serum of children after thermal injury. Adv Med Sci. 2017;62:83-86 pubmed publisher
Shukla N, Somwar R, Smith R, Ambati S, Munoz S, Merchant M, et al. Proteasome Addiction Defined in Ewing Sarcoma Is Effectively Targeted by a Novel Class of 19S Proteasome Inhibitors. Cancer Res. 2016;76:4525-34 pubmed publisher
Liang Q, Dexheimer T, Zhang P, Rosenthal A, Villamil M, You C, et al. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nat Chem Biol. 2014;10:298-304 pubmed publisher

product information

brand :

R&D Systems, powered by Boston Biochem

master code :

E-340

SKU :

E-340-025

product name :

Recombinant Human UCH-L1/PGP9.5 Protein, CF

description :

The Recombinant Human UCH-L1/PGP9.5 Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UCH-L1/PGP9.5 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

UCH-L1 / PGP9.5

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

X mg/ml (X µM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM EDTA, 2 mM DTT

conjugate :

Unconjugated

main entrez gene id :

7345

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

species :

Human

theoretical molecular weight :

25 kDa

gene symbol :

UCHL1

details of functionality :

Recombinant Human UCH-L1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UCH-L1 concentration of 10-50 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.

catalog number base :

E-340

accessionNumbers :

P09936

applications :

Enzyme Activity

source :

The Recombinant Human UCH-L1 / PGP9.5 Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human UCH-L1 / PGP9.5 Protein, CF has been validated for the following applications: Enzyme Activity.

USD :

125 USD

alt names :

EC 3.4.19.12, EC 6.-, Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, PGP9.5Uch-L1, PGP95, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin C-terminal hydrolase, Ubiquitin thioesterase L1, UCHL1, UCH-L1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.