Recombinant Human UCH-L3 Protein, CF | R&D Systems E-325-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human UCH-L3 Protein, CF

catalog :

E-325-025

quantity :

25 ug

more info or order :

R&D Systems product webpage

citations: 4

Reference
Sato H, Ito T, Hayashi T, Kitano S, Erdjument Bromage H, Bott M, et al. The BAP1 nuclear deubiquitinase is involved in the nonhomologous end-joining pathway of double-strand DNA repair through interaction with DNA-PK. Oncogene. 2024;43:1087-1097 pubmed publisher
Su Z, Cao J, Wu Y, Chen W, Lin X, Wu Y, et al. Deubiquitylation of hepatitis B virus X protein (HBx) by ubiquitin-specific peptidase 15 (USP15) increases HBx stability and its transactivation activity. Sci Rep. 2017;7:40246 pubmed publisher
Shukla N, Somwar R, Smith R, Ambati S, Munoz S, Merchant M, et al. Proteasome Addiction Defined in Ewing Sarcoma Is Effectively Targeted by a Novel Class of 19S Proteasome Inhibitors. Cancer Res. 2016;76:4525-34 pubmed publisher
Liang Q, Dexheimer T, Zhang P, Rosenthal A, Villamil M, You C, et al. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nat Chem Biol. 2014;10:298-304 pubmed publisher

product information

master code :

E-325

SKU :

E-325-025

product name :

Recombinant Human UCH-L3 Protein, CF

unit size :

25 ug

description :

The Recombinant Human UCH-L3 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human UCH-L3 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

UCH-L3

category :

Proteins and Enzymes

buffer :

Supplied as a solution in HEPES, NaCl and TCEP.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.

species :

Human

theoretical molecular weight :

26 kDa

gene symbol :

UCHL3

details of functionality :

Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UCH-L3 concentration of 0.05-5 nM. Pre-incubation for 15 minutes with 10 mM DTT is recommended to achieve maximum activity.

accessionNumbers :

P15374.1

applications :

Enzyme Activity

source long :

E. coli -derived human UCH-L3 protein Met1 - Ala230

source short :

E. coli

extended description :

Will be discontinued when existing inventory is gone.

product details :

Will be discontinued when existing inventory is gone.

alt names :

EC 3.4.19.12, ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase), ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, ubiquitin thiolesterase, UCHL3, UCH-L3

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage