Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF | R&D Systems E-313 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF

catalog :

E-313

quantity :

25 ug

price :

265 USD

citations: 5

Reference
Valleau D, Quaile A, Cui H, Xu X, Evdokimova E, Chang C, et al. Discovery of Ubiquitin Deamidases in the Pathogenic Arsenal of Legionella pneumophila. Cell Rep. 2018;23:568-583 pubmed publisher
Zhou H, Lu J, Liu L, Bernard D, Yang C, Fernandez Salas E, et al. A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation. Nat Commun. 2017;8:1150 pubmed publisher
Coleman K, BÃ©kÃ©s M, Chapman J, Crist S, Jones M, Ueberheide B, et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. elife. 2017;6: pubmed publisher
Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, et al. The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as a feedback regulator of biochemical and oncogenic activity. J Biol Chem. 2015;290:296-309 pubmed publisher
Huang G, Stock C, Bommelje C, Weeda V, Shah K, Bains S, et al. SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1). J Biol Chem. 2014;289:34728-42 pubmed publisher

product information

brand :

R&D Systems, powered by Boston Biochem

master code :

E-313

SKU :

E-313-025

product name :

Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF

description :

The Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF has been validated for the following applications: Enzyme Activity.

target :

NEDD8 Activating Enzyme (APPBP1 / UBA3)

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Supplied as a solution in HEPES and NaCl.

conjugate :

Unconjugated

main entrez gene id :

8883

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

species :

Human

theoretical molecular weight :

60 kDa (APP-BP1) . 52 kDa (UBA3)

gene symbol :

NAE1

details of functionality :

Recombinant Human NEDD8 E1 (APP-BP1/UBA3) is a member of the NEDD8-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a NEDD8-conjugating (E2) enzyme and a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human NEDD8 E1 (APP-BP1/UBA3) concentration of 50-200 nM.

catalog number base :

E-313

accessionNumbers :

Q13564 (APP-BP1) Q8TBC4 (UBA3)

applications :

Enzyme Activity

source :

The Recombinant Human NEDD8 E1 (APPBP1 / UBA3) Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human NEDD8 E1 (APPBP1 / UBA3) Protein, CF has been validated for the following applications: Enzyme Activity.

USD :

265 USD

alt names :

A-116A10.1, amyloid beta precursor protein binding protein 1, amyloid beta precursor protein binding protein 1, 59kDa, Amyloid beta precursor protein-binding protein 1, 59 kDa, amyloid beta precursor protein-binding protein 1, 59kD, Amyloid protein-binding protein 1, APPBP1APP-BP1, NEDD8 activating enzyme E1 subunit 1, NEDD8-activating enzyme E1 regulatory subunit, NEDD8-activating enzyme E1 subunit, protooncogene protein 1, Proto-oncogene protein 1, ula-1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.