Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF | R&D Systems E-307-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF

catalog :

E-307-025

quantity :

25 ug

more info or order :

R&D Systems product webpage

citations: 3

Reference
Yan W, Zhong Y, Hu X, Xu T, Zhang Y, Kales S, et al. Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes. Nat Commun. 2023;14:4798 pubmed publisher
Cathcart A, Bird G, Wales T, Herce H, Harvey E, Hauseman Z, et al. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer. Nat Chem Biol. 2020;16:1218-1226 pubmed publisher
Xu X, Keshwani M, Meyer K, Sarikas A, Taylor S, Pan Z. Identification of the degradation determinants of insulin receptor substrate 1 for signaling cullin-RING E3 ubiquitin ligase 7-mediated ubiquitination. J Biol Chem. 2012;287:40758-66 pubmed publisher

product information

master code :

E-307

SKU :

E-307-025

product name :

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF

unit size :

25 ug

description :

The Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF from R&D Systems is derived from Sf 9 (baculovirus). The Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF has been validated for the following applications: Enzyme Activity.

target :

Ubiquitin E1 Enzyme/UBE1L2

category :

Proteins and Enzymes

buffer :

Supplied as a solution in HEPES and DTT. See Certificate of Analysis for details.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.

species :

Human

theoretical molecular weight :

148 kDa

details of functionality :

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) concentration of 50-200 nM.

accessionNumbers :

ABR25253.1

applications :

Enzyme Activity

source long :

Spodoptera frugiperda , Sf 9 (baculovirus)-derived human Ubiquitin E1 Enzyme/UBE1L2 protein Met1 - Asp1052 with a N-terminal GST (glutathione S-transferase) tag

source short :

Sf 9 (baculovirus)

extended description :

Will be discontinued when existing inventory is gone.

product details :

Will be discontinued when existing inventory is gone.

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage