Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF

catalog :

E-306

quantity :

50 ug

price :

219 USD

citations: 4

Reference
Kaneko M, Iwase I, Yamasaki Y, Takai T, Wu Y, Kanemoto S, et al. Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation. Sci Rep. 2016;6:30955 pubmed publisher
Zhao Y, Zhang T, Huo H, Ye Y, Liu Y. Lunapark Is a Component of a Ubiquitin Ligase Complex Localized to the Endoplasmic Reticulum Three-way Junctions. J Biol Chem. 2016;291:18252-62 pubmed publisher
Rodrigo Brenni M, Gutierrez E, Hegde R. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell. 2014;55:227-37 pubmed publisher
Pan Y, Li R, Meng J, Mao H, Zhang Y, Zhang J. Smurf2 negatively modulates RIG-I-dependent antiviral response by targeting VISA/MAVS for ubiquitination and degradation. J Immunol. 2014;192:4758-64 pubmed publisher

product information

brand :

R&D Systems, powered by Boston Biochem

master code :

E-306

SKU :

E-306-050

product name :

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF

description :

The Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF from R&D Systems, powered by Boston Biochem is derived from Sf 9 (baculovirus). The Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Ubiquitin-activating Enzyme / UBE1

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 50 mM NaCl

conjugate :

Unconjugated

main entrez gene id :

7317

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

species :

Human

theoretical molecular weight :

148 kDa

gene symbol :

UBA1

details of functionality :

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) concentration of 50-200 nM.

catalog number base :

E-306

accessionNumbers :

P22314

applications :

Enzyme Activity

source :

USD :

219 USD

alt names :

A1S9T, A1S9T and BN75 temperature sensitivity complementing, A1ST, AMCX1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, UBA1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1A, UBE1, UBE1A1S9, UBE1X, Ubiquitinactivating Enzyme, Ubiquitin-activating enzyme E1, ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing), ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier-activating enzyme 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.