Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF | R&D Systems E-306-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF

catalog :

E-306-050

quantity :

50 ug

price :

330 USD

more info or order :

R&D Systems product webpage

citations: 7

Reference
Wang S, Li H, Liu X, Yin T, Li T, Zheng M, et al. VHL suppresses UBE3B-mediated breast tumor growth and metastasis. Cell Death Dis. 2024;15:446 pubmed publisher
Kong L, Sui C, Chen T, Zhang L, Zhao W, Zheng Y, et al. The ubiquitin E3 ligase TRIM10 promotes STING aggregation and activation in the Golgi apparatus. Cell Rep. 2023;42:112306 pubmed publisher
Kiss L, Clift D, Renner N, Neuhaus D, James L. RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination. Nat Commun. 2021;12:1220 pubmed publisher
Kaneko M, Iwase I, Yamasaki Y, Takai T, Wu Y, Kanemoto S, et al. Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation. Sci Rep. 2016;6:30955 pubmed publisher
Zhao Y, Zhang T, Huo H, Ye Y, Liu Y. Lunapark Is a Component of a Ubiquitin Ligase Complex Localized to the Endoplasmic Reticulum Three-way Junctions. J Biol Chem. 2016;291:18252-62 pubmed publisher
Rodrigo Brenni M, Gutierrez E, Hegde R. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell. 2014;55:227-37 pubmed publisher
Pan Y, Li R, Meng J, Mao H, Zhang Y, Zhang J. Smurf2 negatively modulates RIG-I-dependent antiviral response by targeting VISA/MAVS for ubiquitination and degradation. J Immunol. 2014;192:4758-64 pubmed publisher

product information

master code :

E-306

SKU :

E-306-050

product name :

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF

unit size :

50 ug

description :

The Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF from R&D Systems is derived from Sf 9 (baculovirus). The Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Ubiquitin-activating Enzyme/UBE1

category :

Proteins and Enzymes

buffer :

Supplied as a solution in HEPES NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.

species :

Human

theoretical molecular weight :

148 kDa

gene symbol :

UBA1

details of functionality :

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1) concentration of 50-200 nM.

accessionNumbers :

P22314.3

applications :

Enzyme Activity

source long :

Spodoptera frugiperda , Sf 9 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein Met1 - Arg1058 with a N-terminal GST (glutathione S-transferase) tag

source short :

Sf 9 (baculovirus)

USD :

330 USD

alt names :

A1S9T, A1S9T and BN75 temperature sensitivity complementing, A1ST, AMCX1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, UBA1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1A, UBE1, UBE1A1S9, UBE1X, Ubiquitinactivating Enzyme, Ubiquitin-activating enzyme E1, ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing), ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier-activating enzyme 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage