This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF
catalog :
E-305
quantity :
25 ug
price :
125 USD
citations: 82
Reference
Yoo Y, Park Y, Lee H, Oanh N, Cho M, Heo J, et al. Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesis. Cell Death Dis. 2019;10:938 pubmed publisher
Ting T, Goralski M, Klein K, Wang B, Kim J, Xie Y, et al. Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15. Cell Rep. 2019;29:1499-1510.e6 pubmed publisher
Sitz J, Blanchet S, Gameiro S, Biquand E, Morgan T, Galloy M, et al. Human papillomavirus E7 oncoprotein targets RNF168 to hijack the host DNA damage response. Proc Natl Acad Sci U S A. 2019;116:19552-19562 pubmed publisher
Vaughan R, Rothbart S, Dickson B. The finger loop of the SRA domain in the E3 ligase UHRF1 is a regulator of ubiquitin targeting and is required for the maintenance of DNA methylation. J Biol Chem. 2019;294:15724-15732 pubmed publisher
Wang P, Yan F, Li Z, Yu Y, Parnell S, Xiong Y. Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development. J Clin Invest. 2019;130:4393-4407 pubmed publisher
Ma P, Song N, Li Y, Zhang Q, Zhang L, Zhang L, et al. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning. Cell Rep. 2019;28:541-553.e4 pubmed publisher
Liang F, Miller A, Longerich S, Tang C, Maranon D, Williamson E, et al. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nat Commun. 2019;10:2849 pubmed publisher
Di Rienzo M, Antonioli M, Fusco C, Liu Y, Mari M, Orhon I, et al. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains. Sci Adv. 2019;5:eaau8857 pubmed publisher
Yin Q, Han T, Fang B, Zhang G, Zhang C, Roberts E, et al. K27-linked ubiquitination of BRAF by ITCH engages cytokine response to maintain MEK-ERK signaling. Nat Commun. 2019;10:1870 pubmed publisher
Yip M, Keszei A, Feng Q, Chu V, McKenna M, Shao S. Mechanism for recycling tRNAs on stalled ribosomes. Nat Struct Mol Biol. 2019;26:343-349 pubmed publisher
Lu G, Yi J, Gubas A, Wang Y, Wu Y, Ren Y, et al. Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation. Autophagy. 2019;:1-18 pubmed publisher
Liang Z, Liang F, Teng Y, Chen X, Liu J, Longerich S, et al. Binding of FANCI-FANCD2 Complex to RNA and R-Loops Stimulates Robust FANCD2 Monoubiquitination. Cell Rep. 2019;26:564-572.e5 pubmed publisher
Liu B, Yi J, Yang X, Liu L, Lou X, Zhang Z, et al. MDM2-mediated degradation of WRN promotes cellular senescence in a p53-independent manner. Oncogene. 2019;38:2501-2515 pubmed publisher
Dong Y, Zhang S, Wu Z, Li X, Wang W, Zhu Y, et al. Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome. Nature. 2019;565:49-55 pubmed publisher
Huang X, Wang X, Yuan X, Wu W, Lobie P, Wu Z. XIAP facilitates breast and colon carcinoma growth via promotion of p62 depletion through ubiquitination-dependent proteasomal degradation. Oncogene. 2019;38:1448-1460 pubmed publisher
Lee J, Choi S, Lee Y, Khim K, Yoon S, Kim B, et al. The E3 ubiquitin ligase TRIM25 regulates adipocyte differentiation via proteasome-mediated degradation of PPARγ. Exp Mol Med. 2018;50:135 pubmed publisher
Matyskiela M, Couto S, Zheng X, Lu G, Hui J, Stamp K, et al. SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrate. Nat Chem Biol. 2018;14:981-987 pubmed publisher
Lampert F, Stafa D, Goga A, Soste M, Gilberto S, Olieric N, et al. The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation. elife. 2018;7: pubmed publisher
Ito S, Ueno A, Ueda T, Nakagawa H, Taniguchi H, Kayukawa N, et al. CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells. Oncotarget. 2018;9:17645-17655 pubmed publisher
Khanna R, Krishnamoorthy V, Parnaik V. E3 ubiquitin ligase RNF123 targets lamin B1 and lamin-binding proteins. FEBS J. 2018;285:2243-2262 pubmed publisher
Hewawasam G, Dhatchinamoorthy K, Mattingly M, Seidel C, Gerton J. Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast. Nucleic Acids Res. 2018;46:4440-4455 pubmed publisher
Cho Y, Kang H, Kim S, Lee S, Jee S, Ahn S, et al. Post-translational modification of OCT4 in breast cancer tumorigenesis. Cell Death Differ. 2018;25:1781-1795 pubmed publisher
Zheng L, Conner S. PI5P4K? functions in DTX1-mediated Notch signaling. Proc Natl Acad Sci U S A. 2018;115:E1983-E1990 pubmed publisher
Higa M, Tanaka K, Saijo M. Inhibition of UVSSA ubiquitination suppresses transcription-coupled nucleotide excision repair deficiency caused by dissociation from USP7. FEBS J. 2018;285:965-976 pubmed publisher
Chen X, He Z, Fu M, Wang Y, Wu H, Li X, et al. The E3 Ubiquitin Ligase Siah-1 Suppresses Avian Reovirus Infection by Targeting p10 for Degradation. J Virol. 2018;92: pubmed publisher
Kim S, Kim H, Kim H, Kim D, Han J, Byeon H, et al. HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer. Autophagy. 2018;14:385-403 pubmed publisher
Brickner J, Soll J, Lombardi P, Vågbø C, Mudge M, Oyeniran C, et al. A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair. Nature. 2017;551:389-393 pubmed publisher
Kopp Y, Lang W, Schuster T, Martínez Limón A, Hofbauer H, Ernst R, et al. CHIP as a membrane-shuttling proteostasis sensor. elife. 2017;6: pubmed publisher
Lian N, Liu X, Wang X, Zhou Y, Li H, Li J, et al. COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation. Proc Natl Acad Sci U S A. 2017;114:12321-12326 pubmed publisher
Ma Q, Ruan H, Peng L, Zhang M, Gack M, Yao W. Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity. Proc Natl Acad Sci U S A. 2017;114:E8760-E8769 pubmed publisher
Wang S, Juan J, Zhang Z, Du Y, Xu Y, Tong J, et al. Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis. Cell Death Dis. 2017;8:e3058 pubmed publisher
Zhang B, Holmlund M, Lorrain S, Norberg M, Bako L, Fankhauser C, et al. BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance. elife. 2017;6: pubmed publisher
Janouskova H, El Tekle G, Bellini E, Udeshi N, Rinaldi A, Ulbricht A, et al. Opposing effects of cancer-type-specific SPOP mutants on BET protein degradation and sensitivity to BET inhibitors. Nat Med. 2017;23:1046-1054 pubmed publisher
Murali A, Shin J, Yurugi H, Krishnan A, Akutsu M, Carpy A, et al. Ubiquitin-dependent regulation of Cdc42 by XIAP. Cell Death Dis. 2017;8:e2900 pubmed publisher
Fan Z, Chen J, Kuang J, Lu W, Shan W. The Banana Fruit SINA Ubiquitin Ligase MaSINA1 Regulates the Stability of MaICE1 to be Negatively Involved in Cold Stress Response. Front Plant Sci. 2017;8:995 pubmed publisher
Bononi A, Giorgi C, Patergnani S, Larson D, Verbruggen K, Tanji M, et al. BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature. 2017;546:549-553 pubmed publisher
Inano S, Sato K, Katsuki Y, Kobayashi W, Tanaka H, Nakajima K, et al. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol Cell. 2017;66:622-634.e8 pubmed publisher
Glorian V, Allègre J, Berthelet J, Dumetier B, Boutanquoi P, Droin N, et al. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues. Cell Death Dis. 2017;8:e2816 pubmed publisher
Schunter S, Villa R, Flynn V, Heidelberger J, Classen A, Beli P, et al. Ubiquitylation of the acetyltransferase MOF in Drosophila melanogaster. PLoS ONE. 2017;12:e0177408 pubmed publisher
Xu X, Kathare P, Pham V, Bu Q, Nguyen A, Huq E. Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlies photomorphogenic development in Arabidopsis. Development. 2017;144:1831-1840 pubmed publisher
Perea Resa C, Rodriguez Milla M, Iniesto E, Rubio V, Salinas J. Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis thaliana by Promoting Nuclear Proteasome-Mediated HY5 Degradation. Mol Plant. 2017;10:791-804 pubmed publisher
Wei X, Wang X, Zhan J, Chen Y, Fang W, Zhang L, et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. J Cell Biol. 2017;216:1455-1471 pubmed publisher
Wang J, Yu H, Xiong G, Lu Z, Jiao Y, Meng X, et al. Tissue-Specific Ubiquitination by IPA1 INTERACTING PROTEIN1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice. Plant Cell. 2017;29:697-707 pubmed publisher
Kim J, Shin S, Seo J, Lee E, Jeong M, Lee M, et al. C-terminus of HSC70-Interacting Protein (CHIP) Inhibits Adipocyte Differentiation via Ubiquitin- and Proteasome-Mediated Degradation of PPARγ. Sci Rep. 2017;7:40023 pubmed publisher
Lang X, Tang T, Jin T, Ding C, Zhou R, Jiang W. TRIM65-catalized ubiquitination is essential for MDA5-mediated antiviral innate immunity. J Exp Med. 2017;214:459-473 pubmed publisher
Braganza A, Li J, Zeng X, Yates N, Dey N, Andrews J, et al. UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J Biol Chem. 2017;292:2470-2484 pubmed publisher
Tripathi E, Smith S. Cell cycle-regulated ubiquitination of tankyrase 1 by RNF8 and ABRO1/BRCC36 controls the timing of sister telomere resolution. EMBO J. 2017;36:503-519 pubmed publisher
Nazio F, Carinci M, Valacca C, Bielli P, Strappazzon F, Antonioli M, et al. Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation. J Cell Biol. 2016;215:841-856 pubmed
McClurg U, Cork D, Darby S, Ryan Munden C, Nakjang S, Mendes Côrtes L, et al. Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity. Nucleic Acids Res. 2017;45:1793-1804 pubmed publisher
Lu W, Liu S, Li B, Xie Y, Izban M, Ballard B, et al. SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer. Oncogene. 2017;36:1364-1373 pubmed publisher
Liu H, Li X, Ning G, Zhu S, Ma X, Liu X, et al. The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53. PLoS Biol. 2016;14:e2000733 pubmed publisher
Li L, Cao Y, Wu H, Ye X, Zhu Z, Xing G, et al. Enzymatic Activity of the Scaffold Protein Rapsyn for Synapse Formation. Neuron. 2016;92:1007-1019 pubmed publisher
Xie N, Ma L, Zhu F, Zhao W, Tian F, Yuan F, et al. Regulation of the MDM2-p53 pathway by the nucleolar protein CSIG in response to nucleolar stress. Sci Rep. 2016;6:36171 pubmed publisher
Rose N, King H, Blackledge N, Fursova N, Ember K, Fischer R, et al. RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes. elife. 2016;5: pubmed publisher
Mulvaney K, Matson J, Siesser P, Tamir T, Goldfarb D, Jacobs T, et al. Identification and Characterization of MCM3 as a Kelch-like ECH-associated Protein 1 (KEAP1) Substrate. J Biol Chem. 2016;291:23719-23733 pubmed
Ibarra R, Sandoval D, Fredrickson E, Gardner R, Kleiger G. The San1 Ubiquitin Ligase Functions Preferentially with Ubiquitin-conjugating Enzyme Ubc1 during Protein Quality Control. J Biol Chem. 2016;291:18778-90 pubmed publisher
Matyskiela M, Lu G, Ito T, Pagarigan B, Lu C, Miller K, et al. A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase. Nature. 2016;535:252-7 pubmed publisher
Raychaudhuri K, Chaudhary N, Gurjar M, D Souza R, Limzerwala J, Maddika S, et al. 14-3-3? Gene Loss Leads to Activation of the Epithelial to Mesenchymal Transition Due to the Stabilization of c-Jun Protein. J Biol Chem. 2016;291:16068-81 pubmed publisher
Link L, Howley B, Hussey G, Howe P. PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Mol Cancer Res. 2016;14:634-46 pubmed publisher
Qiu J, Sheedlo M, Yu K, Tan Y, Nakayasu E, Das C, et al. Ubiquitination independent of E1 and E2 enzymes by bacterial effectors. Nature. 2016;533:120-4 pubmed publisher
Zhang H, Liu H, Chen Y, Yang X, Wang P, Liu T, et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016;7:10201 pubmed publisher
Kim C, Yun N, Lee J, Youdim M, Ju C, Kim W, et al. Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated neuronal death. Cell Death Differ. 2016;23:333-46 pubmed publisher
Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, et al. Deltex1 antagonizes HIF-1α and sustains the stability of regulatory T cells in vivo. Nat Commun. 2015;6:6353 pubmed publisher
Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, et al. Tumour suppressor TRIM33 targets nuclear β-catenin degradation. Nat Commun. 2015;6:6156 pubmed publisher
Rout M, Hodge C, Markin C, Xu X, Glover J, Xiao W, et al. Stochastic gate dynamics regulate the catalytic activity of ubiquitination enzymes. J Am Chem Soc. 2014;136:17446-58 pubmed publisher
Yue S, Tang L, Tang Y, Tang Y, Shen Q, Ding J, et al. Requirement of Smurf-mediated endocytosis of Patched1 in sonic hedgehog signal reception. elife. 2014;3: pubmed publisher
Kumar R, Whitehurst C, Pagano J. The Rad6/18 ubiquitin complex interacts with the Epstein-Barr virus deubiquitinating enzyme, BPLF1, and contributes to virus infectivity. J Virol. 2014;88:6411-22 pubmed publisher
Townley Tilson W, Wu Y, Ferguson J, Patterson C. The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2. PLoS ONE. 2014;9:e89451 pubmed publisher
Lonskaya I, Desforges N, Hebron M, Moussa C. Ubiquitination increases parkin activity to promote autophagic ?-synuclein clearance. PLoS ONE. 2013;8:e83914 pubmed publisher
Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, et al. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation. FASEB J. 2014;28:1938-46 pubmed publisher
Liu W, Shang Y, Zeng Y, Liu C, Li Y, Zhai L, et al. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains. EMBO J. 2014;33:46-61 pubmed publisher
Kim J, Park K, Lee E, Jang W, Seo J, Shin S, et al. Suppression of PPAR? through MKRN1-mediated ubiquitination and degradation prevents adipocyte differentiation. Cell Death Differ. 2014;21:594-603 pubmed publisher
Lee H, Alpi A, Park M, Rose A, Koo H. C. elegans ring finger protein RNF-113 is involved in interstrand DNA crosslink repair and interacts with a RAD51C homolog. PLoS ONE. 2013;8:e60071 pubmed publisher
Rajbhandari P, Schalper K, Solodin N, Ellison Zelski S, Ping Lu K, Rimm D, et al. Pin1 modulates ERα levels in breast cancer through inhibition of phosphorylation-dependent ubiquitination and degradation. Oncogene. 2014;33:1438-47 pubmed publisher
Singer A, Schulze S, Skarina T, Xu X, Cui H, Eschen Lippold L, et al. A pathogen type III effector with a novel E3 ubiquitin ligase architecture. PLoS Pathog. 2013;9:e1003121 pubmed publisher
Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, et al. Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G. PLoS Pathog. 2012;8:e1003085 pubmed publisher
Bosch D, Siderovski D. Structural determinants of ubiquitin conjugation in Entamoeba histolytica. J Biol Chem. 2013;288:2290-302 pubmed publisher
Lee T, Park J, Leem S, Kang T. Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair. Oncogene. 2014;33:19-25 pubmed publisher
Wang J, Muntean A, Wu L, Hess J. A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity. J Biol Chem. 2012;287:43410-6 pubmed publisher
Varfolomeev E, Moradi E, Dynek J, Zha J, Fedorova A, Deshayes K, et al. Characterization of ML-IAP protein stability and physiological role in vivo. Biochem J. 2012;447:427-36 pubmed publisher
Pelisch F, Pozzi B, Risso G, Muñoz M, Srebrow A. DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylation regulates p53 transcriptional activation. J Biol Chem. 2012;287:30789-99 pubmed publisher
Bell J, Malyukova A, Holien J, Koach J, Parker M, Kavallaris M, et al. TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members. PLoS ONE. 2012;7:e37470 pubmed publisher
product information
brand :
R&D Systems, powered by Boston Biochem
master code :
E-305
SKU :
E-305-025
product name :
Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF
description :
The Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF from R&D Systems, powered by Boston Biochem is derived from Sf 21 (baculovirus). The Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF has been validated for the following applications: Enzyme Activity.
target :
Ubiquitin-activating Enzyme / UBE1
category :
Proteins and Enzymes
unit size :
25 ug
buffer :
X mg/ml (X µM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 1 mM TCEP
conjugate :
Unconjugated
main entrez gene id :
7317
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
species :
Human
theoretical molecular weight :
118 kDa
gene symbol :
UBA1
details of functionality :
Recombinant Human Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.
catalog number base :
E-305
accessionNumbers :
P22314
applications :
Enzyme Activity
source :
The Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF from R&D Systems, powered by Boston Biochem is derived from Sf 21 (baculovirus). The Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF has been validated for the following applications: Enzyme Activity.
USD :
125 USD
alt names :
A1S9T, A1S9T and BN75 temperature sensitivity complementing, A1ST, AMCX1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, UBA1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1A, UBE1, UBE1A1S9, UBE1X, Ubiquitinactivating Enzyme, Ubiquitin-activating enzyme E1, ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing), ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier-activating enzyme 1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.