product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF
catalog :
E-305-025
quantity :
25 ug
price :
190 USD
more info or order :
citations: 137
Reference
Yao Y, Hong S, Yoshida S, Swaroop V, Curtin B, Inoki K. The Cullin3-Rbx1-KLHL9 E3 ubiquitin ligase complex ubiquitinates Rheb and supports amino acid-induced mTORC1 activation. Cell Rep. 2025;44:115101 pubmed publisher
Tang X, Mei Y, He K, Liu R, Lv X, Zhao Y, et al. The RING-type E3 ligase RIE1 sustains leaf longevity by specifically targeting AtACS7 to fine-tune ethylene production in Arabidopsis. Proc Natl Acad Sci U S A. 2024;121:e2411271121 pubmed publisher
Lu J, Yu J, Liu P, Gu J, Chen Y, Zhang T, et al. Ubiquitin-mediated degradation of SlPsbS regulates low night temperature tolerance in tomatoes. Cell Rep. 2024;43:114757 pubmed publisher
Shi H, Yin J, Zhao Z, Yu H, Yi H, Xu L, et al. Fine-tuning of IPA1 transactivation activity by E3 ligase IPI7-mediated non-proteolytic K29-ubiquitination during Magnaporthe oryzae infection. Nat Commun. 2024;15:7608 pubmed publisher
Zhang R, Wu Y, Qu X, Yang W, Wu Q, Huang L, et al. The RING-finger ubiquitin E3 ligase TaPIR1 targets TaHRP1 for degradation to suppress chloroplast function. Nat Commun. 2024;15:6905 pubmed publisher
Alc xf3 n P, Kaczmarczyk A, Ray K, Liolios T, Guilbaud G, Sijacki T, et al. FANCD2-FANCI surveys DNA and recognizes double- to single-stranded junctions. Nature. 2024;632:1165-1173 pubmed publisher
Kubori T, Arasaki K, Oide H, Kitao T, Nagai H. Multi-tiered actions of Legionella effectors to modulate host Rab10 dynamics. elife. 2024;12: pubmed publisher
Radko Juettner S, Yue H, Myers J, Carter R, Robertson A, Mittal P, et al. Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF. Nature. 2024;628:442-449 pubmed publisher
Wu S, Hu C, Zhu C, Fan Y, Zhou J, Xia X, et al. The MYC2-PUB22-JAZ4 module plays a crucial role in jasmonate signaling in tomato. Mol Plant. 2024;17:598-613 pubmed publisher
Olazabal Herrero A, He B, Kwon Y, Gupta A, Dutta A, Huang Y, et al. The FANCI/FANCD2 complex links DNA damage response to R-loop regulation through SRSF1-mediated mRNA export. Cell Rep. 2024;43:113610 pubmed publisher
Sabri N, Cuneo M, Marzahn M, Lee J, Bouchard J, G xfc ll xfc l xfc x, et al. Reduction of oligomer size modulates the competition between cluster formation and phase separation of the tumor suppressor SPOP. J Biol Chem. 2023;299:105427 pubmed publisher
Xu Y, Qu M, He Y, He Q, Shen T, Luo J, et al. Smurf1 polyubiquitinates on K285/K282 of the kinases Mst1/2 to attenuate their tumor-suppressor functions. J Biol Chem. 2023;299:105395 pubmed publisher
Li P, Zhen Y, Kim C, Liu Z, Hao J, Deng H, et al. Nimbolide targets RNF114 to induce the trapping of PARP1 and synthetic lethality in BRCA-mutated cancer. Sci Adv. 2023;9:eadg7752 pubmed publisher
VanDyke D, Xu L, Sargunas P, Gilbreth R, Baca M, Gao C, et al. Redirecting the specificity of tripartite motif containing-21 scaffolds using a novel discovery and design approach. J Biol Chem. 2023;299:105381 pubmed publisher
Sun J, Huang S, Lu Q, Li S, Zhao S, Zheng X, et al. UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean. Nat Commun. 2023;14:6262 pubmed publisher
Li Y, Bao L, Zheng H, Geng M, Chen T, Dai X, et al. E3 ubiquitin ligase TRIM21 targets TIF1γ to regulate β-catenin signaling in glioblastoma. Theranostics. 2023;13:4919-4935 pubmed publisher
Meyers M, Cismoski S, Panidapu A, Chie Leon B, Nomura D. Targeted Protein Degradation through Recruitment of the CUL4A Complex Adaptor Protein DDB1. bioRxiv. 2023;: pubmed publisher
Yan W, Zhong Y, Hu X, Xu T, Zhang Y, Kales S, et al. Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes. Nat Commun. 2023;14:4798 pubmed publisher
Li Y, Wang H, Sun B, Su G, Cang Y, Zhao L, et al. Smurf1 and Smurf2 mediated polyubiquitination and degradation of RNF220 suppresses Shh-group medulloblastoma. Cell Death Dis. 2023;14:494 pubmed publisher
Fang Z, Li X, Yoshino Y, Suzuki M, Qi H, Murooka H, et al. Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation. Cell Rep. 2023;42:112850 pubmed publisher
Chen M, Wang K, Han Y, Yan S, Yuan H, Liu Q, et al. Identification of XAF1 as an endogenous AKT inhibitor. Cell Rep. 2023;42:112690 pubmed publisher
Zhou R, Chen J, Xu Y, Ye Y, Zhong G, Chen T, et al. PRPF19 facilitates colorectal cancer liver metastasis through activation of the Src-YAP1 pathway via K63-linked ubiquitination of MYL9. Cell Death Dis. 2023;14:258 pubmed publisher
Xu X, Chang C, Li M, Omabe K, Le N, Chen Y, et al. DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation. Nat Commun. 2023;14:1233 pubmed publisher
Gingerich M, Zhu J, Chai B, Vincent M, Xie N, Sidarala V, et al. Reciprocal regulatory balance within the CLEC16A-RNF41 mitophagy complex depends on an intrinsically disordered protein region. J Biol Chem. 2023;299:103057 pubmed publisher
Mevissen T, Prasad A, Walter J. TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away. Cell Rep. 2023;42:112125 pubmed publisher
Best K, Ikeuchi K, Kater L, Best D, Musial J, Matsuo Y, et al. Structural basis for clearing of ribosome collisions by the RQT complex. Nat Commun. 2023;14:921 pubmed publisher
Du X, Pang J, Gu B, Si T, Chang Y, Li T, et al. A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma. Nucleic Acids Res. 2023;51:1050-1066 pubmed publisher
Wei W, Yang Y, Chen J, Lakshmanan P, Kuang J, Lu W, et al. MaNAC029 modulates ethylene biosynthesis and fruit quality and undergoes MaXB3-mediated proteasomal degradation during banana ripening. J Adv Res. 2023;53:33-47 pubmed publisher
Lin L, Lu Y, Huang H, Chen H, Hsu S, Chang L. Regulation of Epstein-Barr Virus Minor Capsid Protein BORF1 by TRIM5α. Int J Mol Sci. 2022;23: pubmed publisher
Liu T, Yang H, Zhuo F, Yang Z, Zhao M, Guo Q, et al. Atypical E3 ligase ZFP91 promotes small-molecule-induced E2F2 transcription factor degradation for cancer therapy. EBioMedicine. 2022;86:104353 pubmed publisher
Kochenova O, Mukkavalli S, Raman M, Walter J. Cooperative assembly of p97 complexes involved in replication termination. Nat Commun. 2022;13:6591 pubmed publisher
Laneri F, Garc xed a Vi xf1 uales S, Lanza V, Licciardello N, Milardi D, Sortino S, et al. Dipyridamole for tracking amyloidogenic proteins aggregation and enhancing polyubiquitination. Arch Biochem Biophys. 2022;728:109354 pubmed publisher
Ma X, Ma X, Zhu L, Zhao Y, Chen M, Li T, et al. The E3 ubiquitin ligase MG53 inhibits hepatocellular carcinoma by targeting RAC1 signaling. Oncogenesis. 2022;11:40 pubmed publisher
Lao L, Bourdeau I, Gagliardi L, He X, Shi W, Hao B, et al. ARMC5 is part of an RPB1-specific ubiquitin ligase implicated in adrenal hyperplasia. Nucleic Acids Res. 2022;: pubmed publisher
Pirooznia S, Wang H, Panicker N, Kumar M, Neifert S, Dar M, et al. Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease. Sci Adv. 2022;8:eabh1824 pubmed publisher
Zhou Y, Zheng R, Liu S, Disoma C, Du A, Li S, et al. Host E3 ligase HUWE1 attenuates the proapoptotic activity of the MERS-CoV accessory protein ORF3 by promoting its ubiquitin-dependent degradation. J Biol Chem. 2022;298:101584 pubmed publisher
Yoon S, Bogdanov K, Wallach D. Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell Death Differ. 2022;: pubmed publisher
Won M, Park K, Kim S, Ju E, Ko Y, Yoo H, et al. ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-κB. Cell Death Differ. 2022;29:1152-1163 pubmed publisher
Mahlokozera T, Patel B, Chen H, Desouza P, Qu X, Mao D, et al. Competitive binding of E3 ligases TRIM26 and WWP2 controls SOX2 in glioblastoma. Nat Commun. 2021;12:6321 pubmed publisher
Manne R, Agrawal Y, Malonia S, Banday S, Edachery S, Patel A, et al. FBXL20 promotes breast cancer malignancy by inhibiting apoptosis through degradation of PUMA and BAX. J Biol Chem. 2021;297:101253 pubmed publisher
Liu Y, Qin C, Rao Y, Ngo C, Feng J, Zhao J, et al. SARS-CoV-2 Nsp5 Demonstrates Two Distinct Mechanisms Targeting RIG-I and MAVS To Evade the Innate Immune Response. MBio. 2021;12:e0233521 pubmed publisher
Kokic G, Wagner F, Chernev A, Urlaub H, Cramer P. Structural basis of human transcription-DNA repair coupling. Nature. 2021;598:368-372 pubmed publisher
Shen C, Nayak A, Neitzel L, Adams A, Silver Isenstadt M, Sawyer L, et al. The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling. Nat Commun. 2021;12:5263 pubmed publisher
Sun Y, Chen J, Huang S, Su Y, Wang W, Agama K, et al. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation. Nat Commun. 2021;12:5010 pubmed publisher
Davies C, Vidal S, Phu L, Sudhamsu J, Hinkle T, Chan Rosenberg S, et al. Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W. Nat Commun. 2021;12:4608 pubmed publisher
Shukla S, Ying W, Gray F, Yao Y, Simes M, Zhao Q, et al. Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain. Nat Chem Biol. 2021;17:784-793 pubmed publisher
McCormack N, Abera M, Arnold E, Gibbs R, Martin S, Buehler E, et al. A high-throughput genome-wide RNAi screen identifies modifiers of survival motor neuron protein. Cell Rep. 2021;35:109125 pubmed publisher
Simoneschi D, Róna G, Zhou N, Jeong Y, Jiang S, Milletti G, et al. CRL4AMBRA1 is a master regulator of D-type cyclins. Nature. 2021;592:789-793 pubmed publisher
Barrows J, Fullbright G, Long D. BRCA1-BARD1 regulates transcription through BRD4 in Xenopus nucleoplasmic extract. Nucleic Acids Res. 2021;49:3263-3273 pubmed publisher
Liebl M, Moehlenbrink J, Becker H, Raddatz G, Abdeen S, Aqeilan R, et al. DAZAP2 acts as specifier of the p53 response to DNA damage. Nucleic Acids Res. 2021;49:2759-2776 pubmed publisher
Huang D, Li Q, Sun X, Sun X, Tang Y, Qu Y, et al. CRL4DCAF8 dependent opposing stability control over the chromatin remodeler LSH orchestrates epigenetic dynamics in ferroptosis. Cell Death Differ. 2021;28:1593-1609 pubmed publisher
Lü X, Shi X, Hu A, Wang J, Ding Y, Jiang W, et al. Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis. Nature. 2020;588:479-484 pubmed publisher
Albert M, Brinkmann K, Pokrzywa W, Günther S, Kronke M, Hoppe T, et al. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage. Cell Death Dis. 2020;11:740 pubmed publisher
Gatti M, Imhof R, Huang Q, Baudis M, Altmeyer M. The Ubiquitin Ligase TRIP12 Limits PARP1 Trapping and Constrains PARP Inhibitor Efficiency. Cell Rep. 2020;32:107985 pubmed publisher
Słabicki M, Kozicka Z, Petzold G, Li Y, Manojkumar M, Bunker R, et al. The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K. Nature. 2020;: pubmed publisher
Matyskiela M, Clayton T, Zheng X, Mayne C, Tran E, Carpenter A, et al. Crystal structure of the SALL4-pomalidomide-cereblon-DDB1 complex. Nat Struct Mol Biol. 2020;27:319-322 pubmed publisher
Yoo Y, Park Y, Lee H, Oanh N, Cho M, Heo J, et al. Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesis. Cell Death Dis. 2019;10:938 pubmed publisher
Ting T, Goralski M, Klein K, Wang B, Kim J, Xie Y, et al. Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15. Cell Rep. 2019;29:1499-1510.e6 pubmed publisher
Sitz J, Blanchet S, Gameiro S, Biquand E, Morgan T, Galloy M, et al. Human papillomavirus E7 oncoprotein targets RNF168 to hijack the host DNA damage response. Proc Natl Acad Sci U S A. 2019;116:19552-19562 pubmed publisher
Vaughan R, Rothbart S, Dickson B. The finger loop of the SRA domain in the E3 ligase UHRF1 is a regulator of ubiquitin targeting and is required for the maintenance of DNA methylation. J Biol Chem. 2019;294:15724-15732 pubmed publisher
Wang P, Yan F, Li Z, Yu Y, Parnell S, Xiong Y. Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development. J Clin Invest. 2019;130:4393-4407 pubmed publisher
Ma P, Song N, Li Y, Zhang Q, Zhang L, Zhang L, et al. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning. Cell Rep. 2019;28:541-553.e4 pubmed publisher
Liang F, Miller A, Longerich S, Tang C, Maranon D, Williamson E, et al. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nat Commun. 2019;10:2849 pubmed publisher
Di Rienzo M, Antonioli M, Fusco C, Liu Y, Mari M, Orhon I, et al. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains. Sci Adv. 2019;5:eaau8857 pubmed publisher
Yin Q, Han T, Fang B, Zhang G, Zhang C, Roberts E, et al. K27-linked ubiquitination of BRAF by ITCH engages cytokine response to maintain MEK-ERK signaling. Nat Commun. 2019;10:1870 pubmed publisher
Yip M, Keszei A, Feng Q, Chu V, McKenna M, Shao S. Mechanism for recycling tRNAs on stalled ribosomes. Nat Struct Mol Biol. 2019;26:343-349 pubmed publisher
Lu G, Yi J, Gubas A, Wang Y, Wu Y, Ren Y, et al. Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation. Autophagy. 2019;:1-18 pubmed publisher
Liang Z, Liang F, Teng Y, Chen X, Liu J, Longerich S, et al. Binding of FANCI-FANCD2 Complex to RNA and R-Loops Stimulates Robust FANCD2 Monoubiquitination. Cell Rep. 2019;26:564-572.e5 pubmed publisher
Liu B, Yi J, Yang X, Liu L, Lou X, Zhang Z, et al. MDM2-mediated degradation of WRN promotes cellular senescence in a p53-independent manner. Oncogene. 2019;38:2501-2515 pubmed publisher
Dong Y, Zhang S, Wu Z, Li X, Wang W, Zhu Y, et al. Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome. Nature. 2019;565:49-55 pubmed publisher
Lee J, Choi S, Lee Y, Khim K, Yoon S, Kim B, et al. The E3 ubiquitin ligase TRIM25 regulates adipocyte differentiation via proteasome-mediated degradation of PPARγ. Exp Mol Med. 2018;50:135 pubmed publisher
Huang X, Wang X, Yuan X, Wu W, Lobie P, Wu Z. XIAP facilitates breast and colon carcinoma growth via promotion of p62 depletion through ubiquitination-dependent proteasomal degradation. Oncogene. 2019;38:1448-1460 pubmed publisher
Matyskiela M, Couto S, Zheng X, Lu G, Hui J, Stamp K, et al. SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrate. Nat Chem Biol. 2018;14:981-987 pubmed publisher
Lampert F, Stafa D, Goga A, Soste M, Gilberto S, Olieric N, et al. The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation. elife. 2018;7: pubmed publisher
Ito S, Ueno A, Ueda T, Nakagawa H, Taniguchi H, Kayukawa N, et al. CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells. Oncotarget. 2018;9:17645-17655 pubmed publisher
Khanna R, Krishnamoorthy V, Parnaik V. E3 ubiquitin ligase RNF123 targets lamin B1 and lamin-binding proteins. FEBS J. 2018;285:2243-2262 pubmed publisher
Hewawasam G, Dhatchinamoorthy K, Mattingly M, Seidel C, Gerton J. Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast. Nucleic Acids Res. 2018;46:4440-4455 pubmed publisher
Cho Y, Kang H, Kim S, Lee S, Jee S, Ahn S, et al. Post-translational modification of OCT4 in breast cancer tumorigenesis. Cell Death Differ. 2018;25:1781-1795 pubmed publisher
Zheng L, Conner S. PI5P4K? functions in DTX1-mediated Notch signaling. Proc Natl Acad Sci U S A. 2018;115:E1983-E1990 pubmed publisher
Higa M, Tanaka K, Saijo M. Inhibition of UVSSA ubiquitination suppresses transcription-coupled nucleotide excision repair deficiency caused by dissociation from USP7. FEBS J. 2018;285:965-976 pubmed publisher
Chen X, He Z, Fu M, Wang Y, Wu H, Li X, et al. The E3 Ubiquitin Ligase Siah-1 Suppresses Avian Reovirus Infection by Targeting p10 for Degradation. J Virol. 2018;92: pubmed publisher
Kim S, Kim H, Kim H, Kim D, Han J, Byeon H, et al. HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer. Autophagy. 2018;14:385-403 pubmed publisher
Brickner J, Soll J, Lombardi P, Vågbø C, Mudge M, Oyeniran C, et al. A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair. Nature. 2017;551:389-393 pubmed publisher
Kopp Y, Lang W, Schuster T, Martínez Limón A, Hofbauer H, Ernst R, et al. CHIP as a membrane-shuttling proteostasis sensor. elife. 2017;6: pubmed publisher
Lian N, Liu X, Wang X, Zhou Y, Li H, Li J, et al. COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation. Proc Natl Acad Sci U S A. 2017;114:12321-12326 pubmed publisher
Zhang B, Holmlund M, Lorrain S, Norberg M, Bako L, Fankhauser C, et al. BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance. elife. 2017;6: pubmed publisher
Janouskova H, El Tekle G, Bellini E, Udeshi N, Rinaldi A, Ulbricht A, et al. Opposing effects of cancer-type-specific SPOP mutants on BET protein degradation and sensitivity to BET inhibitors. Nat Med. 2017;23:1046-1054 pubmed publisher
Murali A, Shin J, Yurugi H, Krishnan A, Akutsu M, Carpy A, et al. Ubiquitin-dependent regulation of Cdc42 by XIAP. Cell Death Dis. 2017;8:e2900 pubmed publisher
Fan Z, Chen J, Kuang J, Lu W, Shan W. The Banana Fruit SINA Ubiquitin Ligase MaSINA1 Regulates the Stability of MaICE1 to be Negatively Involved in Cold Stress Response. Front Plant Sci. 2017;8:995 pubmed publisher
Bononi A, Giorgi C, Patergnani S, Larson D, Verbruggen K, Tanji M, et al. BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature. 2017;546:549-553 pubmed publisher
Inano S, Sato K, Katsuki Y, Kobayashi W, Tanaka H, Nakajima K, et al. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol Cell. 2017;66:622-634.e8 pubmed publisher
Takeda H, Zhou W, Kido K, Suno R, Iwasaki T, Kobayashi T, et al. CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag. PLoS ONE. 2017;12:e0178246 pubmed publisher
Glorian V, Allègre J, Berthelet J, Dumetier B, Boutanquoi P, Droin N, et al. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues. Cell Death Dis. 2017;8:e2816 pubmed publisher
Schunter S, Villa R, Flynn V, Heidelberger J, Classen A, Beli P, et al. Ubiquitylation of the acetyltransferase MOF in Drosophila melanogaster. PLoS ONE. 2017;12:e0177408 pubmed publisher
Xu X, Kathare P, Pham V, Bu Q, Nguyen A, Huq E. Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlies photomorphogenic development in Arabidopsis. Development. 2017;144:1831-1840 pubmed publisher
Perea Resa C, Rodriguez Milla M, Iniesto E, Rubio V, Salinas J. Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis thaliana by Promoting Nuclear Proteasome-Mediated HY5 Degradation. Mol Plant. 2017;10:791-804 pubmed publisher
Wei X, Wang X, Zhan J, Chen Y, Fang W, Zhang L, et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. J Cell Biol. 2017;216:1455-1471 pubmed publisher
Wang J, Yu H, Xiong G, Lu Z, Jiao Y, Meng X, et al. Tissue-Specific Ubiquitination by IPA1 INTERACTING PROTEIN1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice. Plant Cell. 2017;29:697-707 pubmed publisher
Kim J, Shin S, Seo J, Lee E, Jeong M, Lee M, et al. C-terminus of HSC70-Interacting Protein (CHIP) Inhibits Adipocyte Differentiation via Ubiquitin- and Proteasome-Mediated Degradation of PPARγ. Sci Rep. 2017;7:40023 pubmed publisher
Lang X, Tang T, Jin T, Ding C, Zhou R, Jiang W. TRIM65-catalized ubiquitination is essential for MDA5-mediated antiviral innate immunity. J Exp Med. 2017;214:459-473 pubmed publisher
Braganza A, Li J, Zeng X, Yates N, Dey N, Andrews J, et al. UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J Biol Chem. 2017;292:2470-2484 pubmed publisher
Tripathi E, Smith S. Cell cycle-regulated ubiquitination of tankyrase 1 by RNF8 and ABRO1/BRCC36 controls the timing of sister telomere resolution. EMBO J. 2017;36:503-519 pubmed publisher
Nazio F, Carinci M, Valacca C, Bielli P, Strappazzon F, Antonioli M, et al. Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation. J Cell Biol. 2016;215:841-856 pubmed
McClurg U, Cork D, Darby S, Ryan Munden C, Nakjang S, Mendes Côrtes L, et al. Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity. Nucleic Acids Res. 2017;45:1793-1804 pubmed publisher
Lu W, Liu S, Li B, Xie Y, Izban M, Ballard B, et al. SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer. Oncogene. 2017;36:1364-1373 pubmed publisher
Liu H, Li X, Ning G, Zhu S, Ma X, Liu X, et al. The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53. PLoS Biol. 2016;14:e2000733 pubmed publisher
Li L, Cao Y, Wu H, Ye X, Zhu Z, Xing G, et al. Enzymatic Activity of the Scaffold Protein Rapsyn for Synapse Formation. Neuron. 2016;92:1007-1019 pubmed publisher
Xie N, Ma L, Zhu F, Zhao W, Tian F, Yuan F, et al. Regulation of the MDM2-p53 pathway by the nucleolar protein CSIG in response to nucleolar stress. Sci Rep. 2016;6:36171 pubmed publisher
Rose N, King H, Blackledge N, Fursova N, Ember K, Fischer R, et al. RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes. elife. 2016;5: pubmed publisher
Mulvaney K, Matson J, Siesser P, Tamir T, Goldfarb D, Jacobs T, et al. Identification and Characterization of MCM3 as a Kelch-like ECH-associated Protein 1 (KEAP1) Substrate. J Biol Chem. 2016;291:23719-23733 pubmed
Ibarra R, Sandoval D, Fredrickson E, Gardner R, Kleiger G. The San1 Ubiquitin Ligase Functions Preferentially with Ubiquitin-conjugating Enzyme Ubc1 during Protein Quality Control. J Biol Chem. 2016;291:18778-90 pubmed publisher
Matyskiela M, Lu G, Ito T, Pagarigan B, Lu C, Miller K, et al. A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase. Nature. 2016;535:252-7 pubmed publisher
Raychaudhuri K, Chaudhary N, Gurjar M, D Souza R, Limzerwala J, Maddika S, et al. 14-3-3? Gene Loss Leads to Activation of the Epithelial to Mesenchymal Transition Due to the Stabilization of c-Jun Protein. J Biol Chem. 2016;291:16068-81 pubmed publisher
Link L, Howley B, Hussey G, Howe P. PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Mol Cancer Res. 2016;14:634-46 pubmed publisher
Qiu J, Sheedlo M, Yu K, Tan Y, Nakayasu E, Das C, et al. Ubiquitination independent of E1 and E2 enzymes by bacterial effectors. Nature. 2016;533:120-4 pubmed publisher
Zhang H, Liu H, Chen Y, Yang X, Wang P, Liu T, et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016;7:10201 pubmed publisher
Kim C, Yun N, Lee J, Youdim M, Ju C, Kim W, et al. Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated neuronal death. Cell Death Differ. 2016;23:333-46 pubmed publisher
Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, et al. Deltex1 antagonizes HIF-1α and sustains the stability of regulatory T cells in vivo. Nat Commun. 2015;6:6353 pubmed publisher
Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, et al. Tumour suppressor TRIM33 targets nuclear β-catenin degradation. Nat Commun. 2015;6:6156 pubmed publisher
Rout M, Hodge C, Markin C, Xu X, Glover J, Xiao W, et al. Stochastic gate dynamics regulate the catalytic activity of ubiquitination enzymes. J Am Chem Soc. 2014;136:17446-58 pubmed publisher
Yue S, Tang L, Tang Y, Tang Y, Shen Q, Ding J, et al. Requirement of Smurf-mediated endocytosis of Patched1 in sonic hedgehog signal reception. elife. 2014;3: pubmed publisher
Kumar R, Whitehurst C, Pagano J. The Rad6/18 ubiquitin complex interacts with the Epstein-Barr virus deubiquitinating enzyme, BPLF1, and contributes to virus infectivity. J Virol. 2014;88:6411-22 pubmed publisher
Townley Tilson W, Wu Y, Ferguson J, Patterson C. The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2. PLoS ONE. 2014;9:e89451 pubmed publisher
Lonskaya I, Desforges N, Hebron M, Moussa C. Ubiquitination increases parkin activity to promote autophagic ?-synuclein clearance. PLoS ONE. 2013;8:e83914 pubmed publisher
Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, et al. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation. FASEB J. 2014;28:1938-46 pubmed publisher
Liu W, Shang Y, Zeng Y, Liu C, Li Y, Zhai L, et al. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains. EMBO J. 2014;33:46-61 pubmed publisher
Kim J, Park K, Lee E, Jang W, Seo J, Shin S, et al. Suppression of PPAR? through MKRN1-mediated ubiquitination and degradation prevents adipocyte differentiation. Cell Death Differ. 2014;21:594-603 pubmed publisher
Lee H, Alpi A, Park M, Rose A, Koo H. C. elegans ring finger protein RNF-113 is involved in interstrand DNA crosslink repair and interacts with a RAD51C homolog. PLoS ONE. 2013;8:e60071 pubmed publisher
Rajbhandari P, Schalper K, Solodin N, Ellison Zelski S, Ping Lu K, Rimm D, et al. Pin1 modulates ERα levels in breast cancer through inhibition of phosphorylation-dependent ubiquitination and degradation. Oncogene. 2014;33:1438-47 pubmed publisher
Singer A, Schulze S, Skarina T, Xu X, Cui H, Eschen Lippold L, et al. A pathogen type III effector with a novel E3 ubiquitin ligase architecture. PLoS Pathog. 2013;9:e1003121 pubmed publisher
Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, et al. Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G. PLoS Pathog. 2012;8:e1003085 pubmed publisher
Bosch D, Siderovski D. Structural determinants of ubiquitin conjugation in Entamoeba histolytica. J Biol Chem. 2013;288:2290-302 pubmed publisher
Lee T, Park J, Leem S, Kang T. Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair. Oncogene. 2014;33:19-25 pubmed publisher
Wang J, Muntean A, Wu L, Hess J. A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity. J Biol Chem. 2012;287:43410-6 pubmed publisher
Varfolomeev E, Moradi E, Dynek J, Zha J, Fedorova A, Deshayes K, et al. Characterization of ML-IAP protein stability and physiological role in vivo. Biochem J. 2012;447:427-36 pubmed publisher
Pelisch F, Pozzi B, Risso G, Muñoz M, Srebrow A. DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylation regulates p53 transcriptional activation. J Biol Chem. 2012;287:30789-99 pubmed publisher
Bell J, Malyukova A, Holien J, Koach J, Parker M, Kavallaris M, et al. TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members. PLoS ONE. 2012;7:e37470 pubmed publisher
product information
master code :
E-305
SKU :
E-305-025
product name :
Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF
unit size :
25 ug
description :
The Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF has been validated for the following applications: Enzyme Activity.
target :
Ubiquitin-activating Enzyme/UBE1
category :
Proteins and Enzymes
buffer :
Supplied as a solution in HEPES, NaCl and TCEP.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.
species :
Human
theoretical molecular weight :
118 kDa
gene symbol :
UBA1
details of functionality :
Recombinant Human Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.
accessionNumbers :
P22314.3
applications :
Enzyme Activity
source long :
Spodoptera frugiperda , Sf 21 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein
source short :
Sf 21 (baculovirus)
USD :
190 USD
alt names :
A1S9T, A1S9T and BN75 temperature sensitivity complementing, A1ST, AMCX1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, UBA1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1A, UBE1, UBE1A1S9, UBE1X, Ubiquitinactivating Enzyme, Ubiquitin-activating enzyme E1, ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing), ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier-activating enzyme 1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.