Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF | R&D Systems E-300-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF

catalog :

E-300-050

quantity :

50 ug

more info or order :

R&D Systems product webpage

citations: 6

Reference
Singh S, Yeat N, Wang Y, Lin S, Kuo I, Wu K, et al. PTPN23 ubiquitination by WDR4 suppresses EGFR and c-MET degradation to define a lung cancer therapeutic target. Cell Death Dis. 2023;14:671 pubmed publisher
Radmall K, Shukla P, Leng A, Chandrasekharan M. Structure-function analysis of histone H2B and PCNA ubiquitination dynamics using deubiquitinase-deficient strains. Sci Rep. 2023;13:16731 pubmed publisher
Xu W, Yu Y, Jing J, Wu Z, Zhang X, You C, et al. SCFRMF mediates degradation of the meiosis-specific recombinase DMC1. Nat Commun. 2023;14:5044 pubmed publisher
Shukla P, Bissell J, Kumar S, Pokhrel S, Palani S, Radmall K, et al. Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex. Nucleic Acids Res. 2023;51:2117-2136 pubmed publisher
Shukla P, Sinha D, Leng A, Bissell J, Thatipamula S, Ganguly R, et al. Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126 in helix-3 affect ubiquitination activity and decrease enzyme stability. J Biol Chem. 2022;298:102524 pubmed publisher
Lee S, Tumolo J, EHLINGER A, Jernigan K, Qualls Histed S, Hsu P, et al. Ubiquitin turnover and endocytic trafficking in yeast are regulated by Ser57 phosphorylation of ubiquitin. elife. 2017;6: pubmed publisher

product information

master code :

E-300

SKU :

E-300-050

product name :

Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF

unit size :

50 ug

description :

The Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1), CF has been validated for the following applications: Bioactivity.

target :

Ubiquitin-activating Enzyme/UBE1

category :

Proteins and Enzymes

buffer :

Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.

species :

Yeast

theoretical molecular weight :

141 kDa

gene symbol :

UBA1

details of functionality :

Recombinant Yeast GST-Ubiquitin E1 Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial GST-Ubiquitin E1 Enzyme (UBE1) concentration of 50-200 nM.

accessionNumbers :

P22515.2

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived yeast Ubiquitin-activating Enzyme/UBE1 protein Met1 - Leu1024 with a N-terminal GST (glutathione S-transferase) tag

source short :

Sf 21 (baculovirus)

extended description :

Will be discontinued when existing inventory is gone.

product details :

Will be discontinued when existing inventory is gone.

alt names :

A1S9T, A1S9T and BN75 temperature sensitivity complementing, A1ST, AMCX1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, UBA1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1A, UBE1, UBE1A1S9, UBE1X, Ubiquitinactivating Enzyme, Ubiquitin-activating enzyme E1, ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing), ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier-activating enzyme 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage