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product summary
company name :
R&D Systems
product type :
ELISA/assay
product name :
Human PDGF-AB Quantikine ELISA Kit
catalog :
DHD00C
quantity :
1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
price :
655 USD
citations: 43
Reference
Michalak A, Guz M, Kozicka J, Cybulski M, Jeleniewicz W, Telejko I, et al. miRNAs and Hematological Markers in Non-Alcoholic Fatty Liver Disease-A New Diagnostic Path?. Biomedicines. 2025;13: pubmed publisher
Michalak A, Guz M, Kozicka J, Cybulski M, Jeleniewicz W, Szczygie x142 K, et al. microRNAs and Other Serological Markers of Liver Fibrosis in Patients with Alcohol-Related Liver Cirrhosis. Biomedicines. 2024;12: pubmed publisher
Guo C, Mo R, Kim H. Surface topography modulates initial platelet adhesion to titanium substrata. J Oral Biol Craniofac Res. 2024;14:471-477 pubmed publisher
Smoczer C, Yuth K, Askar M, Young L, Paurazas S. Growth Factors Released from Advanced Platelet-Rich Fibrin in the Presence of Calcium-Based Silicate Materials and Their Impact on the Viability and Migration of Stem Cells of Apical Papilla. Dent J (Basel). 2023;11: pubmed publisher
Kobari L, Auclair M, Piau O, Ferrand N, Zaoui M, Delhommeau F, et al. Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells. Leukemia. 2022;36:540-548 pubmed publisher
Walters B, Turner P, Rolauffs B, Hart M, Stegemann J. Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells. Cells. 2021;10: pubmed publisher
Casagrande N, Borghese C, Agostini F, Durante C, Mazzucato M, Colombatti A, et al. In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel. Int J Mol Sci. 2021;22: pubmed publisher
Michalak A, Cicho x17c Lach H, Guz M, Kozicka J, Cybulski M, Jeleniewicz W. Plateletcrit and Mean Platelet Volume in the Evaluation of Alcoholic Liver Cirrhosis and Nonalcoholic Fatty Liver Disease Patients. Biomed Res Int. 2021;2021:8867985 pubmed publisher
Michalak A, Cichoz Lach H, Guz M, Kozicka J, Cybulski M, Jeleniewicz W, et al. Towards an evaluation of alcoholic liver cirrhosis and nonalcoholic fatty liver disease patients with hematological scales. World J Gastroenterol. 2020;26:7538-7549 pubmed publisher
Pauly S, Klatte Schulz F, Stahnke K, Scheibel M, Wildemann B. The effect of autologous platelet rich plasma on tenocytes of the human rotator cuff. BMC Musculoskelet Disord. 2018;19:422 pubmed publisher
Agostini F, Ruzza M, Corpillo D, Biondi L, Acquadro E, Canepa B, et al. 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures. PLoS ONE. 2018;13:e0203048 pubmed publisher
Rossi S, Mori M, Vigani B, Bonferoni M, Sandri G, Riva F, et al. A novel dressing for the combined delivery of platelet lysate and vancomycin hydrochloride to chronic skin ulcers: Hyaluronic acid particles in alginate matrices. Eur J Pharm Sci. 2018;118:87-95 pubmed publisher
Nagaishi K, Mizue Y, Chikenji T, Otani M, Nakano M, Saijo Y, et al. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy. Sci Rep. 2017;7:8484 pubmed publisher
Shen B, Zhang Z, Zhou N, Huang Y, Bao Y, Wu D, et al. Preparing Platelet-Rich Plasma with Whole Blood Harvested Intraoperatively During Spinal Fusion. Med Sci Monit. 2017;23:3578-3584 pubmed
Bernardi M, Agostini F, Chieregato K, Amati E, Durante C, Rassu M, et al. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro. J Transl Med. 2017;15:90 pubmed publisher
Toth Zsamboki E, Horváth Z, Hajtman L, Leé S, Pallinger E, Kuklis E, et al. Cardiac rehabilitation programme as a non-pharmacological platelet inhibitory tool in acute coronary syndrome survivors. Eur J Prev Cardiol. 2017;24:1148-1156 pubmed publisher
Glovinski P, Herly M, Mathiasen A, Svalgaard J, Borup R, Talman M, et al. Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates. Cytotherapy. 2017;19:222-234 pubmed publisher
Anitua E, de la Fuente M, Ferrando M, Quintana F, Larreategui Z, Matorras R, et al. Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts. Eur J Obstet Gynecol Reprod Biol. 2016;206:125-130 pubmed publisher
Chou C, Modo M. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling. Sci Rep. 2016;6:29029 pubmed publisher
Chou M, Burnouf T, Wang T. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate. PLoS ONE. 2014;9:e99145 pubmed publisher
Kageyama Y, Doi T, Matsushima Nishiwaki R, Iida Y, Akamatsu S, Kondo A, et al. Involvement of Rac in thromboxane A2?induced human platelet activation: regulation of sCD40 ligand release and PDGF?AB secretion. Mol Med Rep. 2014;10:107-12 pubmed publisher
Iudicone P, Fioravanti D, Bonanno G, Miceli M, Lavorino C, Totta P, et al. Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells. J Transl Med. 2014;12:28 pubmed publisher
Barsotti M, Chiara Barsotti M, Losi P, Briganti E, Sanguinetti E, Magera A, et al. Effect of platelet lysate on human cells involved in different phases of wound healing. PLoS ONE. 2013;8:e84753 pubmed publisher
Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol. 2013;14:48 pubmed publisher
Rauch C, Feifel E, Amann E, Spötl H, Schennach H, Pfaller W, et al. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX. 2011;28:305-16 pubmed
Ding W, Knox T, Tschumper R, Wu W, Schwager S, Boysen J, et al. Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch. Blood. 2010;116:2984-93 pubmed publisher
Mathew P, Thall P, Wen S, Bucana C, Jones D, Horne E, et al. Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer. Br J Cancer. 2008;99:1426-32 pubmed publisher
Kanter J, Khan S, Kelher M, Gore L, Silliman C. Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates. Clin Cancer Res. 2008;14:3942-7 pubmed publisher
Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC. Arterioscler Thromb Vasc Biol. 2008;28:135-41 pubmed
Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma. Int J Cancer. 2007;120:686-93 pubmed
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Tejada M, Yu L, Dong J, Jung K, Meng G, Peale F, et al. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Clin Cancer Res. 2006;12:2676-88 pubmed
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Nagineni C, Kutty V, Detrick B, Hooks J. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J Cell Physiol. 2005;203:35-43 pubmed
Saika S, Kono Saika S, Tanaka T, Yamanaka O, Ohnishi Y, Sato M, et al. Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice. Lab Invest. 2004;84:1245-58 pubmed
Thorn J, Sørensen H, Weis Fogh U, Andersen M. Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery. Int J Oral Maxillofac Surg. 2004;33:95-100 pubmed
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Bracamonte M, Rud K, Owen W, Miller V. Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle. Am J Physiol Heart Circ Physiol. 2002;283:H853-60 pubmed
Skutek M, van Griensven M, Zeichen J, Brauer N, Bosch U. Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts. Eur J Appl Physiol. 2001;86:48-52 pubmed
Westphal J, Van t Hullenaar R, Peek R, Willems R, Crickard K, Crickard U, et al. Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo. Int J Cancer. 2000;86:768-76 pubmed
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Soubrier M, Sauron C, Souweine B, Larroche C, Wechsler B, Guillevin L, et al. Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome. Am J Kidney Dis. 1999;34:633-8 pubmed
product information
master code :
DHD00C
SKU :
DHD00C
product name :
Human PDGF-AB Quantikine ELISA Kit
unit size :
1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
description :
The Human PDGF-AB Quantikine ELISA Kit from R&D Systems is a Solid Phase Sandwich ELISA that quantifies human PDGF-AB in cell culture supernates (100 ul), serum (10 ul), platelet-poor edta plasma (100 ul), platelet-poor heparin plasma (100 ul).
target :
PDGF-AB
category :
ELISAs
species :
Human
specificity :
Natural and recombinant human PDGF-AB
kit type :
Solid Phase Sandwich ELISA
assay length :
4.5 hours
elisaSampleTypes :
Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
elisaSensitivity :
3.83 pg/mL
elisaRange :
15.60 - 1000 pg/mL
top caption :
Human PDGF-AB ELISA Cell Culture Supernate Standard Curve
applications :
ELISA
USD :
655 USD
product details :
The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB.
storage :
Store the unopened product at 2 - 8 ░C. Do not use past expiration date.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.