product summary
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company name :
R&D Systems
product type :
ELISA/assay
product name :
Human EGF Quantikine ELISA Kit
catalog :
DEG00
quantity :
1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
price :
655 USD
more info or order :
citations: 76
Reference
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Razani E, Aghayan H, Alavi Moghadam S, Yari F, Gharehbaghian A, Sharifi Z. A comparative study of pathogen inactivation technologies in human platelet lysate and its optimal efficiency in human placenta-derived stem cells culture. J Virol Methods. 2022;302:114478 pubmed publisher
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Li H, Huang C, Lui K, Chao Y, Yeh C, Lee L, et al. Combination of Epithelial Growth Factor Receptor Blockers and CDK4/6 Inhibitor for Nasopharyngeal Carcinoma Treatment. Cancers (Basel). 2021;13: pubmed publisher
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Garvey C, Lau R, Sanchez A, Sun R, Fong E, Doche M, et al. Anti-EGFR Therapy Induces EGF Secretion by Cancer-Associated Fibroblasts to Confer Colorectal Cancer Chemoresistance. Cancers (Basel). 2020;12: pubmed publisher
Di Marco S, Riccitelli S, Di Paolo M, Campos E, Buzzi M, Bisti S, et al. Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas. Biomolecules. 2020;10: pubmed publisher
Dong Z, Zou J, Li J, Pang Y, Liu Y, Deng C, et al. MYST1/KAT8 contributes to tumor progression by activating EGFR signaling in glioblastoma cells. Cancer Med. 2019;8:7793-7808 pubmed publisher
Haque A, Moriyama M, Kubota K, Ishiguro N, Sakamoto M, Chinju A, et al. CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production. Sci Rep. 2019;9:14611 pubmed publisher
Liang Z, Lu X, Zhu D, Yi X, Wu F, He N, et al. Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro. Cell Transplant. 2019;28:286-295 pubmed publisher
Satirapoj B, Dispan R, Radinahamed P, Kitiyakara C. Urinary epidermal growth factor, monocyte chemoattractant protein-1 or their ratio as predictors for rapid loss of renal function in type 2 diabetic patients with diabetic kidney disease. BMC Nephrol. 2018;19:246 pubmed publisher
Agostini F, Ruzza M, Corpillo D, Biondi L, Acquadro E, Canepa B, et al. 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures. PLoS ONE. 2018;13:e0203048 pubmed publisher
Saury C, Lardenois A, Schleder C, Leroux I, Lieubeau B, David L, et al. Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches. Stem Cell Res Ther. 2018;9:128 pubmed publisher
Xing P, Wang H, Yang S, Han X, Sun Y, Shi Y. Therapeutic cancer vaccine: phase I clinical tolerance study of Hu-rhEGF-rP64k/Mont in patients with newly diagnosed advanced non-small cell lung cancer. BMC Immunol. 2018;19:14 pubmed publisher
Azuma N, Katada Y, Sano H. Deterioration in saliva quality in patients with Sjögren's syndrome: impact of decrease in salivary epidermal growth factor on the severity of intraoral manifestations. Inflamm Regen. 2018;38:6 pubmed publisher
Pierce J, Benedetti E, Preslar A, Jacobson P, Jin P, Stroncek D, et al. Comparative analyses of industrial-scale human platelet lysate preparations. Transfusion. 2017;: pubmed publisher
Nagaishi K, Mizue Y, Chikenji T, Otani M, Nakano M, Saijo Y, et al. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy. Sci Rep. 2017;7:8484 pubmed publisher
Go Y, Kim S, Cho G, Chae S, Song J. Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts. PLoS ONE. 2017;12:e0182716 pubmed publisher
Garner A, Caiafa A, Jiang Y, Klopman S, Morton C, Torres A, et al. Design, characterization and experimental validation of a compact, flexible pulsed power architecture for ex vivo platelet activation. PLoS ONE. 2017;12:e0181214 pubmed publisher
Shen L, Zhang P, Zhang S, Xie L, Yao L, Lang W, et al. C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers. Exp Ther Med. 2017;13:3021-3031 pubmed publisher
Chandran S, Watkins J, Abdul Aziz A, Shafat M, Calvert P, Bowles K, et al. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction. J Am Heart Assoc. 2017;6: pubmed publisher
Bernardi M, Agostini F, Chieregato K, Amati E, Durante C, Rassu M, et al. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro. J Transl Med. 2017;15:90 pubmed publisher
Soeda A, Lathia J, Williams B, Wu Q, Gallagher J, Androutsellis Theotokis A, et al. The p38 signaling pathway mediates quiescence of glioma stem cells by regulating epidermal growth factor receptor trafficking. Oncotarget. 2017;8:33316-33328 pubmed publisher
Worawichawong S, Worawichawong S, Radinahamed P, Muntham D, Sathirapongsasuti N, Nongnuch A, et al. Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis. Kidney Blood Press Res. 2016;41:997-1007 pubmed publisher
Glovinski P, Herly M, Mathiasen A, Svalgaard J, Borup R, Talman M, et al. Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates. Cytotherapy. 2017;19:222-234 pubmed publisher
Anitua E, de la Fuente M, Ferrando M, Quintana F, Larreategui Z, Matorras R, et al. Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts. Eur J Obstet Gynecol Reprod Biol. 2016;206:125-130 pubmed publisher
Hang Q, Isaji T, Hou S, Zhou Y, Fukuda T, Gu J. N-Glycosylation of integrin ?5 acts as a switch for EGFR-mediated complex formation of integrin ?5?1 to ?6?4. Sci Rep. 2016;6:33507 pubmed publisher
Saad A, Zhu X, Herrmann S, Hickson L, Tang H, Dietz A, et al. Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia. Stem Cell Res Ther. 2016;7:128 pubmed publisher
Przepiera Bedzak H, Fischer K, Brzosko M. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome. Int J Mol Sci. 2016;17: pubmed publisher
He Y, Schmidt M, Erwin C, Guo J, Sun R, Pendarvis K, et al. Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF). PLoS ONE. 2016;11:e0157034 pubmed publisher
Chou M, Burnouf T, Wang T. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate. PLoS ONE. 2014;9:e99145 pubmed publisher
Zhang Z, Dong Z, Lauxen I, Filho M, Nor J. Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype. Cancer Res. 2014;74:2869-81 pubmed publisher
Dickinson R, Milne P, Jardine L, Zandi S, Swierczek S, McGovern N, et al. The evolution of cellular deficiency in GATA2 mutation. Blood. 2014;123:863-74 pubmed publisher
Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, et al. Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer. Clin Cancer Res. 2012;18:6219-26 pubmed publisher
Feng X, Scheinberg P, Samsel L, Rios O, Chen J, McCoy J, et al. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura. J Thromb Haemost. 2012;10:1616-23 pubmed publisher
Llovet J, Peña C, Lathia C, Shan M, Meinhardt G, Bruix J. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma. Clin Cancer Res. 2012;18:2290-300 pubmed publisher
Rauch C, Feifel E, Amann E, Spötl H, Schennach H, Pfaller W, et al. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX. 2011;28:305-16 pubmed
Rahbari N, Schmidt T, Falk C, Hinz U, Herber M, Bork U, et al. Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer. BMC Cancer. 2011;11:286 pubmed publisher
Aronis K, Diakopoulos K, Fiorenza C, Chamberland J, Mantzoros C. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans. Diabetologia. 2011;54:2358-67 pubmed publisher
Lee H, Myers A, Kim J. Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis. Am J Respir Crit Care Med. 2009;180:1056-67 pubmed publisher
Farrow B, Berger D, Rowley D. Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2. J Surg Res. 2009;156:155-60 pubmed publisher
Sexton D, Chen T, Martik D, Kuzmic P, Kuang G, Chen J, et al. Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases. Biochem J. 2009;422:383-92 pubmed publisher
Soler García A, Rakhmanina N, Mattison P, Ray P. A urinary biomarker profile for children with HIV-associated renal diseases. Kidney Int. 2009;76:207-14 pubmed publisher
Debucquoy A, Haustermans K, Daemen A, Aydin S, Libbrecht L, Gevaert O, et al. Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer. J Clin Oncol. 2009;27:2751-7 pubmed publisher
Han S, Oh D, Im S, Park S, Lee K, Song H, et al. Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer. Br J Cancer. 2009;100:298-304 pubmed publisher
Cherney D, Konvalinka A, Zinman B, Diamandis E, Soosaipillai A, Reich H, et al. Effect of protein kinase Cbeta inhibition on renal hemodynamic function and urinary biomarkers in humans with type 1 diabetes: a pilot study. Diabetes Care. 2009;32:91-3 pubmed publisher
Ren W, Korchin B, Zhu Q, Wei C, Dicker A, Heymach J, et al. Epidermal growth factor receptor blockade in combination with conventional chemotherapy inhibits soft tissue sarcoma cell growth in vitro and in vivo. Clin Cancer Res. 2008;14:2785-95 pubmed publisher
Garcia B, Neninger E, de la Torre A, Leonard I, Martínez R, Viada C, et al. Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth fa. Clin Cancer Res. 2008;14:840-6 pubmed publisher
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Lemos González Y, Rodriguez Berrocal F, Cordero O, Gomez C, Paez de la Cadena M. Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma. Br J Cancer. 2007;96:1569-78 pubmed
Kverka M, Burianova J, Lodinova Zadnikova R, Kocourkova I, Cinova J, Tuckova L, et al. Cytokine profiling in human colostrum and milk by protein array. Clin Chem. 2007;53:955-62 pubmed
Dolloff N, Russell M, Loizos N, Fatatis A. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor. Cancer Res. 2007;67:555-62 pubmed
Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma. Int J Cancer. 2007;120:686-93 pubmed
Groschl M, Topf H, Kratzsch J, Dotsch J, Rascher W, Rauh M. Salivary leptin induces increased expression of growth factors in oral keratinocytes. J Mol Endocrinol. 2005;34:353-66 pubmed
Jahanshahi G, Motavasel V, Rezaie A, Hashtroudi A, Daryani N, Abdollahi M. Alterations in antioxidant power and levels of epidermal growth factor and nitric oxide in saliva of patients with inflammatory bowel diseases. Dig Dis Sci. 2004;49:1752-7 pubmed
Balicki R, Grabowska S, Citko A. Salivary epidermal growth factor in oral cavity cancer. Oral Oncol. 2005;41:48-55 pubmed
Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, et al. A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells. Biochem Biophys Res Commun. 2004;325:24-31 pubmed
Chiou Y, Chiu N, Wang S, Cheng H, Tang M. Factors associated with the outcomes of children with unilateral ureteropelvic junction obstruction. J Urol. 2004;171:397-402; discussion 402 pubmed
Truckenmiller M, Vawter M, Zhang P, Conejero Goldberg C, Dillon Carter O, Morales N, et al. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression. Exp Neurol. 2002;175:318-37 pubmed
Nagler R, Hershkovich O, Lischinsky S, Diamond E, Reznick A. Saliva analysis in the clinical setting: revisiting an underused diagnostic tool. J Investig Med. 2002;50:214-25 pubmed publisher
Benson M, Uddman R, Cardell L. Epithelial cells in nasal fluids from patients with allergic rhinitis: how do they relate to epidermal growth factor, eosinophils and eosinophil cationic protein?. Acta Otolaryngol. 2002;122:202-5 pubmed
Chen C, Yang J, Lin K, Chao K, Ho H, Yang Y. The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx. Hum Reprod. 2002;17:128-33 pubmed
Shin C, Falcone R, Stuart L, Erwin C, Warner B. Diminished epidermal growth factor levels in infants with necrotizing enterocolitis. J Pediatr Surg. 2000;35:173-6; discussion 177 pubmed
image
image 1 :
R&D Systems DEG00 image 1
Human EGF ELISA Cell Culture Supernate/Urine Standard Curve
product information
brand :
R&D Systems
master code :
DEG00
SKU :
DEG00
product name :
Human EGF Quantikine ELISA Kit
unit size :
1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
seo description :
The Human EGF Quantikine ELISA Kit from R&D Systems is a Solid Phase Sandwich ELISA that quantifies human EGF in cell culture supernates (200 ul), serum (10 ul), edta plasma (200 ul), heparin plasma (200 ul), platelet-poor citrate plasma (200 ul), urine (10 ul).
target :
EGF
category :
ELISAs
dilution :
ELISA
species :
Human
specificity :
Natural and recombinant human EGF
gene symbol :
EGF
kit type :
Solid Phase Sandwich ELISA
assay length :
3.5 hours or 4.5 hours
elisaSampleTypes :
Cell Culture Supernates (200 uL), Serum (10 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Platelet-poor Citrate Plasma (200 uL), Urine (10 uL)
elisaSensitivity :
0.7 pg/mL
elisaRange :
3.90 - 250 pg/mL
top caption :
Human EGF ELISA Cell Culture Supernate/Urine Standard Curve
applications :
ELISA
USD :
655 USD
product details :
The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli -derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.
alt names :
beta-urogastrone, epidermal growth factor, epidermal growth factor (beta-urogastrone), hEGF, HOMG4, pro-epidermal growth factor, URG, Urogastrone
storage :
Store the unopened product at 2 - 8 ░C. Do not use past expiration date.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.