This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
ELISA/assay
product name :
Human PDGF-BB Quantikine ELISA Kit
catalog :
DBB00
quantity :
1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
price :
655 USD
citations: 63
Reference
Arai T, Shiga Y, Mukai M, Takayama N, Tashiro S, Tajiri I, et al. Osteogenic effects and safety of human induced pluripotent stem cell-derived megakaryocytes and platelets produced on a clinical scale. Regen Ther. 2024;26:850-858 pubmed publisher
Iacovacci J, Serafini M, Avuzzi B, Badenchini F, Cicchetti A, Devecchi A, et al. Intestinal microbiota composition is predictive of radiotherapy-induced acute gastrointestinal toxicity in prostate cancer patients. EBioMedicine. 2024;106:105246 pubmed publisher
Scovino A, Dahab E, Diniz Lima I, de Senna Silveira E, Barroso S, Cardoso K, et al. A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants. Microorganisms. 2024;12: pubmed publisher
R xed os S, Gonz xe1 lez L, Saez C, Smith P, Escobar L, Mart xed nez C. L-PRF Secretome from Both Smokers/Nonsmokers Stimulates Angiogenesis and Osteoblast Differentiation In Vitro. Biomedicines. 2024;12: pubmed publisher
Mochizuki T, Ushiki T, Suzuki K, Sato M, Ishiguro H, Suwabe T, et al. Elevated IL-1β and Comparable IL-1 Receptor Antagonist Levels Are Characteristic Features of L-PRP in Female College Athletes Compared to Male Professional Soccer Players. Int J Mol Sci. 2023;24: pubmed publisher
Mochizuki T, Ushiki T, Suzuki K, Sato M, Ishiguro H, Suwabe T, et al. Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels. Int J Mol Sci. 2023;24: pubmed publisher
Sch xf6 nborn M, Gregorczyk Maga I, Batko K, Maga M, Bogucka K, Gawlik K, et al. Angiogenic and Microvascular Status Alterations after Endovascular Revascularization of Lower Limb Arteries among Patients with Diabetic Foot Syndrome: A Prospective 12-Month Follow-Up Study. J Clin Med. 2023;12: pubmed publisher
Gregorczyk Maga I, Szustkiewicz Karo x144 A, Gajda M, Kapusta M, Maga W, Sch xf6 nborn M. The Concentration of Pro- and Antiangiogenic Factors in Saliva and Gingival Crevicular Fluid Compared to Plasma in Patients with Peripheral Artery Disease and Type 2 Diabetes. Biomedicines. 2023;11: pubmed publisher
Sch xf6 nborn M, Gregorczyk Maga I, Batko K, Bogucka K, Maga M, P x142 otek A, et al. Circulating Angiogenic Factors and Ischemic Diabetic Foot Syndrome Advancement-A Pilot Study. Biomedicines. 2023;11: pubmed publisher
Ali R, Quinn G, Umarova R, Haddad J, Zhang G, Townsend E, et al. Longitudinal multi-omics analyses of the gut-liver axis reveals metabolic dysregulation in hepatitis C infection and cirrhosis. Nat Microbiol. 2023;8:12-27 pubmed publisher
Fukuda K, Kuroda T, Tamura N, Mita H, Miyata H, Kasashima Y. Platelet Lysate Enhances Equine Skeletal Muscle Regeneration in A Bupivacaine-Induced Muscle Injury Model☆. J Equine Vet Sci. 2022;112:103892 pubmed publisher
Kobari L, Auclair M, Piau O, Ferrand N, Zaoui M, Delhommeau F, et al. Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells. Leukemia. 2022;36:540-548 pubmed publisher
Du S, Yang Z, Lu X, Yousuf S, Zhao M, Li W, et al. Anoikis resistant gastric cancer cells promote angiogenesis and peritoneal metastasis through C/EBPβ-mediated PDGFB autocrine and paracrine signaling. Oncogene. 2021;40:5764-5779 pubmed publisher
Yamazaki K, Kawabori M, Seki T, Takamiya S, Konno K, Watanabe M, et al. Mesenchymal Stem Cell Sheet Promotes Functional Recovery and Palliates Neuropathic Pain in a Subacute Spinal Cord Injury Model. Stem Cells Int. 2021;2021:9964877 pubmed publisher
Isozaki S, Konishi H, Fujiya M, Tanaka H, Murakami Y, Kashima S, et al. Probiotic-Derived Polyphosphate Accelerates Intestinal Epithelia Wound Healing through Inducing Platelet-Derived Mediators. Mediators Inflamm. 2021;2021:5582943 pubmed publisher
Casagrande N, Borghese C, Agostini F, Durante C, Mazzucato M, Colombatti A, et al. In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel. Int J Mol Sci. 2021;22: pubmed publisher
Idemoto K, Ishima T, Niitsu T, Hata T, Yoshida S, Hattori K, et al. Platelet-derived growth factor BB: A potential diagnostic blood biomarker for differentiating bipolar disorder from major depressive disorder. J Psychiatr Res. 2021;134:48-56 pubmed publisher
Liang Y, Deng X, Lin X, Jiang L, Huang X, Mo Z, et al. RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner. Cell Death Dis. 2020;11:855 pubmed publisher
Fukuda K, Kuroda T, Tamura N, Mita H, Kasashima Y. Optimal activation methods for maximizing the concentrations of platelet-derived growth factor-BB and transforming growth factor-β1 in equine platelet-rich plasma. J Vet Med Sci. 2020;82:1472-1479 pubmed publisher
Lin L, Lin J, Yang C, Cheng H, Chang K, Liu C. Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma. Int J Mol Sci. 2020;21: pubmed publisher
Sekine S, Kaneko M, Tanaka M, Ninomiya Y, Kurita H, Inden M, et al. Functional evaluation of PDGFB-variants in idiopathic basal ganglia calcification, using patient-derived iPS cells. Sci Rep. 2019;9:5698 pubmed publisher
Kuo T, Cheng K, Shan Y, Chen L, Hung W. β-catenin-activated autocrine PDGF/Src signaling is a therapeutic target in pancreatic cancer. Theranostics. 2019;9:324-336 pubmed publisher
Notodihardjo S, Morimoto N, Kakudo N, Mitsui T, Le T, Tabata Y, et al. Comparison of the efficacy of cryopreserved human platelet lysate and refrigerated lyophilized human platelet lysate for wound healing. Regen Ther. 2019;10:1-9 pubmed publisher
Agostini F, Ruzza M, Corpillo D, Biondi L, Acquadro E, Canepa B, et al. 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures. PLoS ONE. 2018;13:e0203048 pubmed publisher
Bai M, Chuang M, Lin M, Tang S, Wong C, Chan W. Relationships of Age and Sex with Cytokine Content and Distribution in Human Platelet Fibrin Gels. Sci Rep. 2018;8:10642 pubmed publisher
Togliatto G, Dentelli P, Rosso A, Lombardo G, Gili M, Gallo S, et al. PDGF-BB Carried by Endothelial Cell-Derived Extracellular Vesicles Reduces Vascular Smooth Muscle Cell Apoptosis in Diabetes. Diabetes. 2018;67:704-716 pubmed publisher
Piran M, Vakilian S, Piran M, Mohammadi Sangcheshmeh A, Hosseinzadeh S, Ardeshirylajimi A. In vitro fibroblast migration by sustained release of PDGF-BB loaded in chitosan nanoparticles incorporated in electrospun nanofibers for wound dressing applications. Artif Cells Nanomed Biotechnol. 2018;46:511-520 pubmed publisher
Pierce J, Benedetti E, Preslar A, Jacobson P, Jin P, Stroncek D, et al. Comparative analyses of industrial-scale human platelet lysate preparations. Transfusion. 2017;: pubmed publisher
Bernardi M, Agostini F, Chieregato K, Amati E, Durante C, Rassu M, et al. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro. J Transl Med. 2017;15:90 pubmed publisher
Isobe K, Suzuki M, Watanabe T, Kitamura Y, Suzuki T, Kawabata H, et al. Platelet-rich fibrin prepared from stored whole-blood samples. Int J Implant Dent. 2017;3:6 pubmed publisher
Hauschild G, Geburek F, Gosheger G, Eveslage M, Serrano D, Streitburger A, et al. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations. BMC Vet Res. 2017;13:7 pubmed publisher
Bayraktar S, Jungbluth P, Deenen R, Grassmann J, Schneppendahl J, Eschbach D, et al. Molecular- and microarray-based analysis of diversity among resting and osteogenically induced porcine mesenchymal stromal cells of several tissue origin. J Tissue Eng Regen Med. 2018;12:114-128 pubmed publisher
Tan C, Shichinohe H, Wang Z, Hamauchi S, Abumiya T, Nakayama N, et al. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke. Stem Cells Int. 2016;2016:6104780 pubmed
Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H, et al. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent. 2016;2:19 pubmed
Miao X, Zhang W, Huang Z, Li N. Unaltered Angiogenesis-Regulating Activities of Platelets in Mild Type 2 Diabetes Mellitus despite a Marked Platelet Hyperreactivity. PLoS ONE. 2016;11:e0162405 pubmed publisher
Chou C, Modo M. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling. Sci Rep. 2016;6:29029 pubmed publisher
Dauwe D, Pelacho B, Wibowo A, Walravens A, Verdonck K, Gillijns H, et al. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved. J Am Heart Assoc. 2016;5:e002288 pubmed publisher
Verstraete M, Debucquoy A, Dekervel J, Van Pelt J, Verslype C, Devos E, et al. Combining bevacizumab and chemoradiation in rectal cancer. Translational results of the AXEBeam trial. Br J Cancer. 2015;112:1314-25 pubmed publisher
Paul G, Zachrisson O, Varrone A, Almqvist P, Jerling M, Lind G, et al. Safety and tolerability of intracerebroventricular PDGF-BB in Parkinson's disease patients. J Clin Invest. 2015;125:1339-46 pubmed publisher
Tan H, Giannoudis P, Boxall S, McGonagle D, Jones E. The systemic influence of platelet-derived growth factors on bone marrow mesenchymal stem cells in fracture patients. BMC Med. 2015;13:6 pubmed publisher
Chen C, Feng X, Liao H, Jin W, Zhang J, Wang Y, et al. Regulation of T cell proliferation by JMJD6 and PDGF-BB during chronic hepatitis B infection. Sci Rep. 2014;4:6359 pubmed publisher
Liegl R, Koenig S, Siedlecki J, Haritoglou C, Kampik A, Kernt M. Temsirolimus inhibits proliferation and migration in retinal pigment epithelial and endothelial cells via mTOR inhibition and decreases VEGF and PDGF expression. PLoS ONE. 2014;9:e88203 pubmed publisher
Ricard N, Tu L, Le Hiress M, Huertas A, Phan C, Thuillet R, et al. Increased pericyte coverage mediated by endothelial-derived fibroblast growth factor-2 and interleukin-6 is a source of smooth muscle-like cells in pulmonary hypertension. Circulation. 2014;129:1586-97 pubmed publisher
Zehetner C, Kirchmair R, Neururer S, Kralinger M, Bechrakis N, Kieselbach G. Systemic upregulation of PDGF-B in patients with neovascular AMD. Invest Ophthalmol Vis Sci. 2014;55:337-44 pubmed publisher
Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol. 2013;14:48 pubmed publisher
Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, et al. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells. 2013;31:146-55 pubmed publisher
Mathew P, Thall P, Wen S, Bucana C, Jones D, Horne E, et al. Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer. Br J Cancer. 2008;99:1426-32 pubmed publisher
Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, et al. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis. Eur J Pharmacol. 2008;590:400-8 pubmed publisher
Kilian O, Wenisch S, Karnati S, Baumgart Vogt E, Hild A, Fuhrmann R, et al. Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite. Biomaterials. 2008;29:3429-37 pubmed publisher
Fujii T, Onimaru M, Yonemitsu Y, Kuwano H, Sueishi K. Statins restore ischemic limb blood flow in diabetic microangiopathy via eNOS/NO upregulation but not via PDGF-BB expression. Am J Physiol Heart Circ Physiol. 2008;294:H2785-91 pubmed publisher
Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC. Arterioscler Thromb Vasc Biol. 2008;28:135-41 pubmed
Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, et al. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro. Exp Hematol. 2007;35:1119-31 pubmed
Dolloff N, Russell M, Loizos N, Fatatis A. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor. Cancer Res. 2007;67:555-62 pubmed
Lederle W, Stark H, Skobe M, Fusenig N, Mueller M. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells. Am J Pathol. 2006;169:1767-83 pubmed
Schmidmaier G, Herrmann S, Green J, Weber T, Scharfenberger A, Haas N, et al. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation. Bone. 2006;39:1156-1163 pubmed publisher
Undas A, Celinska Lowenhoff M, Stepien E, Nizankowski R, Tracz W, Szczeklik A. Effects of simvastatin on angiogenic growth factors released at the site of microvascular injury. Thromb Haemost. 2006;95:1045-7 pubmed
Tejada M, Yu L, Dong J, Jung K, Meng G, Peale F, et al. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Clin Cancer Res. 2006;12:2676-88 pubmed
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, et al. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J Immunol. 2005;175:6177-89 pubmed
Dardik A, Yamashita A, Aziz F, Asada H, Sumpio B. Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1alpha. J Vasc Surg. 2005;41:321-31 pubmed
Nagineni C, Kutty V, Detrick B, Hooks J. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J Cell Physiol. 2005;203:35-43 pubmed
Saika S, Kono Saika S, Tanaka T, Yamanaka O, Ohnishi Y, Sato M, et al. Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice. Lab Invest. 2004;84:1245-58 pubmed
Zhou W, Park I, Pins M, Kozlowski J, Jovanovic B, Zhang J, et al. Dual regulation of proliferation and growth arrest in prostatic stromal cells by transforming growth factor-beta1. Endocrinology. 2003;144:4280-4 pubmed
Bracamonte M, Rud K, Owen W, Miller V. Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle. Am J Physiol Heart Circ Physiol. 2002;283:H853-60 pubmed
product information
master code :
DBB00
SKU :
DBB00
product name :
Human PDGF-BB Quantikine ELISA Kit
unit size :
1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
description :
The Human PDGF-BB Quantikine ELISA Kit from R&D Systems is a Solid Phase Sandwich ELISA that quantifies human PDGF-BB in cell culture supernates (100 ul), serum (10 ul), platelet-poor edta plasma (100 ul), platelet-poor heparin plasma (100 ul).
target :
PDGF-BB
category :
ELISAs
species :
Human
specificity :
Natural and recombinant human PDGF-BB
gene symbol :
PDGFB
kit type :
Solid Phase Sandwich ELISA
assay length :
4.5 hours
elisaSampleTypes :
Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
elisaSensitivity :
15 pg/mL
elisaRange :
31.20 - 2000 pg/mL
top caption :
Human PDGF-BB ELISA Cell Culture Supernate Standard Curve
applications :
ELISA
USD :
655 USD
product details :
The Quantikine Human PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-BB in cell culture supernates, serum, or plasma. It contains E. coli -expressed recombinant human PDGF-BB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-BB.
alt names :
c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV, PDGFBB
storage :
Store the unopened product at 2 - 8 ░C. Do not use past expiration date.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.