This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
ELISA/assay
product name :
Human IL-12 p70 Quantikine ELISA Kit
catalog :
D1200
quantity :
1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
price :
655 USD
citations: 76
Reference
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Li Y, Li Y, Yang T, Wang M. Dioscin attenuates oxLDL uptake and the inflammatory reaction of dendritic cells under high glucose conditions by blocking p38 MAPK. Mol Med Rep. 2020;21:304-310 pubmed publisher
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Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, et al. Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses. PLoS ONE. 2014;9:e100166 pubmed publisher
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Necchi A, Pennati M, Zaffaroni N, Landoni E, Giannatempo P, Raggi D, et al. Analysis of plasma cytokines and angiogenic factors in patients with pretreated urothelial cancer receiving Pazopanib: the role of circulating interleukin-8 to enhance the prognostic accuracy. Br J Cancer. 2014;110:26-33 pubmed publisher
Haselow K, Bode J, Wammers M, Ehlting C, Keitel V, Kleinebrecht L, et al. Bile acids PKA-dependently induce a switch of the IL-10/IL-12 ratio and reduce proinflammatory capability of human macrophages. J Leukoc Biol. 2013;94:1253-64 pubmed publisher
Freytag S, Barton K, Zhang Y. Efficacy of oncolytic adenovirus expressing suicide genes and interleukin-12 in preclinical model of prostate cancer. Gene Ther. 2013;20:1131-9 pubmed publisher
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Vyth Dreese F, Sein J, van de Kasteele W, Dellemijn T, van den Bogaard C, Nooijen W, et al. Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma. Clin Exp Immunol. 2010;162:447-59 pubmed publisher
Legat A, Thomas S, Hermand P, Van Mechelen M, Goldman M, De Wit D. CD14-independent responses induced by a synthetic lipid A mimetic. Eur J Immunol. 2010;40:797-802 pubmed publisher
Tieu H, Ananworanich J, Avihingsanon A, Apateerapong W, Sirivichayakul S, Siangphoe U, et al. Immunologic markers as predictors of tuberculosis-associated immune reconstitution inflammatory syndrome in HIV and tuberculosis coinfected persons in Thailand. AIDS Res Hum Retroviruses. 2009;25:1083-9 pubmed publisher
Chase J, Celli J, Bosio C. Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4. Infect Immun. 2009;77:180-95 pubmed publisher
Hsieh C, Lu J, Chen S, Lan C, Chow W, Tang R. Cerebrospinal fluid levels of interleukin-6 and interleukin-12 in children with meningitis. Childs Nerv Syst. 2009;25:461-5 pubmed publisher
Olsnes A, Ryningen A, Ersvaer E, Bruserud Ø. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22. J Interferon Cytokine Res. 2008;28:297-310 pubmed publisher
Liu T, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, et al. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur J Immunol. 2008;38:1012-23 pubmed publisher
Little R, Aleman K, Kumar P, Wyvill K, Pluda J, Read Connole E, et al. Phase 2 study of pegylated liposomal doxorubicin in combination with interleukin-12 for AIDS-related Kaposi sarcoma. Blood. 2007;110:4165-71 pubmed
Frances R, González Navajas J, Zapater P, Munoz C, Cano R, Pascual S, et al. Translocation of bacterial DNA from Gram-positive microorganisms is associated with a species-specific inflammatory response in serum and ascitic fluid of patients with cirrhosis. Clin Exp Immunol. 2007;150:230-7 pubmed
Irhimeh M, Fitton J, Lowenthal R. Fucoidan ingestion increases the expression of CXCR4 on human CD34+ cells. Exp Hematol. 2007;35:989-94 pubmed
Lepej S, Misic Majerus L, Jeren T, Rode O, Remenar A, Sporec V, et al. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis. Acta Neurol Scand. 2007;115:109-14 pubmed
Vaknin Dembinsky A, Balashov K, Weiner H. IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production. J Immunol. 2006;176:7768-74 pubmed
Spensieri F, Fedele G, Fazio C, Nasso M, Stefanelli P, Mastrantonio P, et al. Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction. Infect Immun. 2006;74:2831-8 pubmed
Ferrandina G, Ranelletti F, Legge F, Salutari V, Martinelli E, Fattorossi A, et al. Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer. Clin Cancer Res. 2006;12:2055-60 pubmed
Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, et al. Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma. J Neuroimmunol. 2006;175:169-75 pubmed
Hartl D, Latzin P, Zissel G, Krane M, Krauss Etschmann S, Griese M. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis. Am J Respir Crit Care Med. 2006;173:1370-6 pubmed
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Tsai I, Tsai C, Ho Y, Ho K, Wu Y, Hung C. Interleukin-12 and interleukin-16 in periodontal disease. Cytokine. 2005;31:34-40 pubmed
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Takei M, Umeyama A, Arihara S, Matsumoto H. Effect of piceatannol in human monocyte-derived dendritic cells in vitro. J Pharm Sci. 2005;94:974-82 pubmed
Jensen J, Krakauer M, Sellebjerg F. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b. Cytokine. 2005;29:24-30 pubmed
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Saeki S, Matsuse H, Kondo Y, Machida I, Kawano T, Tomari S, et al. Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients. J Allergy Clin Immunol. 2004;114:538-44 pubmed
Larsson M, Babcock E, Grakoui A, Shoukry N, Lauer G, Rice C, et al. Lack of phenotypic and functional impairment in dendritic cells from chimpanzees chronically infected with hepatitis C virus. J Virol. 2004;78:6151-61 pubmed
Garofalo R, Hintz K, Hill V, Ogra P, Welliver R. Production of interferon gamma in respiratory syncytial virus infection of humans is not associated with interleukins 12 and 18. J Med Virol. 2004;73:289-94 pubmed
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Chaiyaroj S, Rutta A, Muenthaisong K, Watkins P, Na Ubol M, Looareesuwan S. Reduced levels of transforming growth factor-beta1, interleukin-12 and increased migration inhibitory factor are associated with severe malaria. Acta Trop. 2004;89:319-27 pubmed
Vankayalapati R, Klucar P, Wizel B, Weis S, Samten B, Safi H, et al. NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen. J Immunol. 2004;172:130-7 pubmed
Mirani M, Elenkov I, Volpi S, Hiroi N, Chrousos G, Kino T. HIV-1 protein Vpr suppresses IL-12 production from human monocytes by enhancing glucocorticoid action: potential implications of Vpr coactivator activity for the innate and cellular immunity deficits observed in HIV-1 infection. J Immunol. 2002;169:6361-8 pubmed
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Pages F, Lebel Binay S, Vieillefond A, Deneux L, Cambillau M, Soubrane O, et al. Local immunostimulation induced by intravesical administration of autologous interferon-gamma-activated macrophages in patients with superficial bladder cancer. Clin Exp Immunol. 2002;127:303-9 pubmed
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product information
master code :
D1200
SKU :
D1200
product name :
Human IL-12 p70 Quantikine ELISA Kit
unit size :
1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
description :
The Human IL-12 p70 Quantikine ELISA Kit from R&D Systems is a Solid Phase Sandwich ELISA that quantifies human IL-12 p70/IL-12A in cell culture supernates (200 ul), serum (200 ul), edta plasma (200 ul), heparin plasma (200 ul), citrate plasma (200 ul), urine (200 ul).
target :
IL-12 p70
category :
ELISAs
species :
Human
specificity :
Natural and recombinant human IL-12p70
gene symbol :
IL12A
kit type :
Solid Phase Sandwich ELISA
assay length :
3.5 hours or 4.5 hours
elisaSampleTypes :
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL), Urine (200 uL)
elisaSensitivity :
5 pg/mL
elisaRange :
7.80 - 500 pg/mL
top caption :
Human IL-12 p70 ELISA Cell Culture Supernate/Serum/Plasma Standard Curve
applications :
ELISA
USD :
655 USD
product details :
The Quantikine Human IL-12 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-12 in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-12 accurately. Results obtained using natural human IL-12 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12.
alt names :
CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35
storage :
Store the unopened product at 2 - 8 ░C. Do not use past expiration date.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.