Recombinant Human VISTA Fc Chimera Biotinylated Protein, CF | R&D Systems BT7126-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human VISTA Fc Chimera Biotinylated Protein, CF

catalog :

BT7126-050

quantity :

50 ug

price :

624 USD

more info or order :

R&D Systems product webpage

citations: 1

Reference
Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, et al. VSIG-3 as a ligand of VISTA inhibits human T-cell function. Immunology. 2019;156:74-85 pubmed publisher

image

image 1 :

Recombinant Human VISTA Fc Chimera Biotinylated Protein Binding Activity

product information

master code :

BT7126

SKU :

BT7126-050

product name :

Recombinant Human VISTA Fc Chimera Biotinylated Protein, CF

unit size :

50 ug

description :

The Recombinant Human VISTA Fc Chimera Biotinylated Protein, CF from R&D Systems is derived from NS0. The Recombinant Human VISTA Fc Chimera Biotinylated Protein, CF has been validated for the following applications: Bioactivity.

target :

VISTA/B7-H5/PD-1H

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose.

conjugate :

Biotin

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

64 - 75 kDa, reducing conditions

theoretical molecular weight :

45 kDa (unlabeled)

details of functionality :

Measured by its binding ability in a functional ELISA. When Recombinant Human VSIG-3 Fc Chimera (Catalog # 9229-VS ) is immobilized at 5 ╡g/mL, 100 ╡L/well, the concentration of Biotinylated Recombinant VISTA/B7-H5/PD-1H Fc Chimera that produces 50% of the optimal binding response is 1-6 ╡g/mL. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 1-6 ╡g/m

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human VISTA Fc Chimera Biotinylated Protein Binding Activity

accessionNumbers :

AAH20568

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human VISTA/B7-H5/PD-1H protein Human VISTA/B7-H5/PD-1H (Phe33-Ala194) Accession # AAH20568 IEGRMD Human IgG1 (Pro100-Lys330) N-terminus

source short :

NS0

USD :

624 USD

alt names :

4632428N05Rik, B7H5, B7-H5, C10orf54, chromosome 10 open reading frame 54, Dies1, Gi24, PD1H, PD-1H, platelet receptor Gi24, PP2135, SISP1, stress induced secreted protein 1, VSIR

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage