Recombinant Human Dkk-1 Biotinylated Protein | R&D Systems BT5439-010 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Dkk-1 Biotinylated Protein

catalog :

BT5439-010

quantity :

10 ug

price :

536 USD

citations: 1

Reference
Priestley R, Cheung J, Murphy E, Ehebauer M, Davis J, Di Daniel E. A novel high-content imaging-based technique for measuring binding of Dickkopf-1 to low-density lipoprotein receptor-related protein 6. J Pharmacol Toxicol Methods. 2019;95:47-55 pubmed publisher

product information

master code :

BT5439

SKU :

BT5439-010

product name :

Recombinant Human Dkk-1 Biotinylated Protein

unit size :

10 ug

description :

The Recombinant Human Dkk-1 Biotinylated Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Human Dkk-1 Biotinylated Protein has been validated for the following applications: Bioactivity.

target :

Dkk-1

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Biotin

purity :

>95%, by SDS-PAGE with silver staining.

species :

Human

observed molecular weight :

33-38 kDa, reducing conditions

theoretical molecular weight :

26 kDa (unlabeled)

gene symbol :

DKK1

details of functionality :

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Biotinylated Recombinant Human Dkk-1 (Catalog # BT5439) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a (Catalog # 5036-WN ). The ED50 for this effect is 10-60 ng/mL.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human Dkk-1 Biotinylated Protein Bioactivity

accessionNumbers :

O94907

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived human Dkk-1 protein Thr32-His266

source short :

Sf 21 (baculovirus)

USD :

536 USD

alt names :

dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1, Dickkopf-1, dickkopf-related protein 1, Dkk1, DKK-1, hDkk-1, SKdickkopf-1 like

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.