Recombinant Human TNF-alpha, Biotinylated Protein | R&D Systems BT210-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human TNF-alpha, Biotinylated Protein

catalog :

BT210-010

quantity :

10 ug

price :

373 USD

more info or order :

R&D Systems product webpage

citations: 6

Reference
Joseph N, Shapiro A, Gillis E, Barkey S, Abu Horowitz A, Bachelet I, et al. Biodistribution and function of coupled polymer-DNA origami nanostructures. Sci Rep. 2023;13:19567 pubmed publisher
Zhang Z, Kong X, Ligtenberg M, van Hal van Veen S, Visser N, de Bruijn B, et al. RNF31 inhibition sensitizes tumors to bystander killing by innate and adaptive immune cells. Cell Rep Med. 2022;3:100655 pubmed publisher
Fritsch J, Frankenheim J, Marischen L, Vadasz T, Troeger A, Rose John S, et al. Roles for ADAM17 in TNF-R1 Mediated Cell Death and Survival in Human U937 and Jurkat Cells. Cells. 2021;10: pubmed publisher
Elliott P, Leske D, Wagstaff J, Schlicher L, Berridge G, Maslen S, et al. Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains. Cell Rep. 2021;37:109777 pubmed publisher
Wong S, Lenzini S, Cooper M, Mooney D, Shin J. Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and trafficking. Sci Adv. 2020;6:eaaw0158 pubmed publisher
Holdbrooks A, Britain C, Bellis S. ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor. J Biol Chem. 2018;293:1610-1622 pubmed publisher

image

image 1 :

Recombinant Human TNF-alpha, Biotinylated Protein Bioactivity

product information

master code :

BT210

SKU :

BT210-010

product name :

Recombinant Human TNF-alpha, Biotinylated Protein

unit size :

10 ug

description :

The Recombinant Human TNF-alpha, Biotinylated Protein from R&D Systems is derived from E. coli. The Recombinant Human TNF-alpha, Biotinylated Protein has been validated for the following applications: Bioactivity.

target :

TNF-alpha

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Biotin

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

17 kDa, reducing conditions

theoretical molecular weight :

17 kDa (unlabeled)

gene symbol :

TNF

details of functionality :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycináD. Matthews,áN. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human TNF-alpha, Biotinylated Protein Bioactivity

accessionNumbers :

P01375

applications :

Bioactivity

source long :

E. coli -derived human TNF-alpha protein Val77-Leu233, with and without an N-terminal Met

source short :

E. coli

USD :

373 USD

alt names :

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage