protein

Recombinant Human IL-2 Biotinylated Protein

BT202-025

25 ug

815 USD

BT202-025

Recombinant Human IL-2 Biotinylated Protein

25 ug

The Recombinant Human IL-2 Biotinylated Protein from R&D Systems is derived from E. coli. The Recombinant Human IL-2 Biotinylated Protein has been validated for the following applications: Bioactivity.

IL-2

Proteins and Enzymes

Lyophilized from a 0.2 ╡m filtered solution in HCl with BSA as a carrier protein.

Biotin

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

Human

13 kDa, reducing conditions

15 kDa (unlabeled)

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-2 R alpha Fc Chimera (Catalog # 1020-RL ) is immobilized at 1 ╡g/mL (100 ╡L/well), Biotinylated Recombinant Human IL-2 binds with an ED50 of 4-24 ng/mL. Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL.

<0.10 EU per 1 ╡g of the protein by the LAL method.

Recombinant Human IL-2 Biotinylated Protein Binding Activity

P60568

Bioactivity

E. coli -derived human IL-2 protein Ala21-Thr153, with an N-terminal Met

E. coli

815 USD

aldesleukin, IL2, IL-2, IL-2lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, Proleukin, T cell growth factor, T-cell growth factor, TCGF

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.