product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse Wnt-3a, Biotinylated Protein, CF
catalog :
BT1324-010/CF
quantity :
10 ug
price :
1465 USD
more info or order :
R&D Systems product webpage
citations: 1
Reference
Yun J, Hansen S, Morris O, Madden D, Libeu C, Kumar A, et al. Senescent cells perturb intestinal stem cell differentiation through Ptk7 induced noncanonical Wnt and YAP signaling. Nat Commun. 2023;14:156 pubmed publisher
image
image 1 :
R&D Systems BT1324-010/CF image 1
Recombinant Mouse Wnt-3a, Biotinylated Protein Binding Activity
product information
master code :
BT1324/CF
SKU :
BT1324-010/CF
product name :
Recombinant Mouse Wnt-3a, Biotinylated Protein, CF
unit size :
10 ug
description :
The Recombinant Mouse Wnt-3a, Biotinylated Protein, CF from R&D Systems is derived from CHO. The Recombinant Mouse Wnt-3a, Biotinylated Protein, CF has been validated for the following applications: Bioactivity.
target :
Wnt-3a
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS, EDTA and CHAPS.
conjugate :
Biotin
purity :
>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Mouse
theoretical molecular weight :
37 kDa (unlabeled)
details of functionality :
Measured by its binding ability in a functional ELISA. In a 100 ╡L reaction mixture containing biotinylated Recombinant Mouse Wnt-3a at 50 ng/mL and Recombinant Human Frizzled-5 Fc Chimera (Catalog # 1617-FZC ) dilutions, the concentration of Recombinant Human Frizzled-5 Fc Chimera that produces 50% of the maximal binding response is 2-12 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Mouse Wnt-3a, Biotinylated Protein Binding Activity
accessionNumbers :
P27467
applications :
Bioactivity
source long :
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-3a protein Ser19-Lys352
source short :
CHO
USD :
1465 USD
alt names :
MGC119418, MGC119419, MGC119420, protein Wnt-3a, wingless-type MMTV integration site family, member 3A, Wnt3a
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.