protein

Recombinant Human VEGF 165 GMP Protein, CF

BT-VEGF-GMP-050

50 ug (also 1 mg)

742 USD

BT-VEGF-GMP-050

Recombinant Human VEGF 165 GMP Protein, CF

50 ug (also 1 mg)

The Recombinant Human VEGF 165 GMP Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human VEGF 165 GMP Protein, CF has been validated for the following applications: Bioactivity.

VEGF

Proteins and Enzymes

Lyophilized from a 0.2 ╡m filtered solution in Sodium Acetate.

Unconjugated

97%, by SDS-PAGE with quantitative densitometry by Coomassie« Blue Staining. The molecular weight by TCEP reduced mass spectrometry is 19282 ▒ 10 Da and 19080 Da ▒ 10 Da, and is free of unexpected mass peaks

Human

19 - 21 kDa, under reducing conditions.

19.2 kDa (monomer)

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al . (1990) Proc. Natl. Acad. Sci. USA 87 :1323. The ED50 for this effect is 1.50-12.0 ng/mL. The specific activity of recombinant human VEGF165 is 8.0 x 105 units/mg, which is calibrated against the human VEGF165 WHO standard (NIBSC code: 02/286).

<0.01 EU per 1 ╡g of the protein by the LAL method.

NP_001165097.1

Bioactivity

E. coli -derived human VEGF protein Ala27 - Arg191 Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.

E. coli

Animal-Free

742 USD

Animal-Free

MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at <= -20 ░C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, <= -20 ░C under sterile conditions after reconstitution.