protein

Recombinant Human FGF basic/FGF2/bFGF GMP Protein, CF

BT-FGFB-GMP-025

25 ug (also 1 mg)

308 USD

BT-FGFB-GMP-025

Recombinant Human FGF basic/FGF2/bFGF GMP Protein, CF

25 ug (also 1 mg)

The Recombinant Human FGF basic/FGF2/bFGF GMP Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human FGF basic/FGF2/bFGF GMP Protein, CF has been validated for the following applications: Bioactivity.

FGF basic/FGF2/bFGF

Proteins and Enzymes

Lyophilized from a 0.2 ╡m filtered solution in HEPES and Sodium Sulfate with Trehalose.

Unconjugated

97%, by SDS-PAGE with quantitative densitometry by Coomassie« Blue Staining. The primary molecular weight by mass spectrometry is 17112 Da ▒ 10 Da

Human

18 kDa, under reducing conditions.

17 kDa

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al . (1985) Methods Enzymol. 109 :749. The ED50 for this effect is 0.100-1.00 ng/mL. The specific activity of Recombinant Human FGF basic/FGF2 GMP is 8.0 x 105 IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).

<0.01 EU per 1 ╡g of the protein by the LAL method.

NP_001997.5

Bioactivity

E. coli -derived human FGF basic/FGF2/bFGF protein Ala135 - Ser288 Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.

E. coli

308 USD

Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at <= -20 ░C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, <= -20 ░C under sterile conditions after reconstitution.