Human VCAM-1/CD106 Antibody | R&D Systems BBA5 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human VCAM-1/CD106 Antibody

catalog :

BBA5

quantity :

100 ug

price :

439 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BBIG-V1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

R&D Systems product webpage

citations: 53

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:1000; loading ...; fig 3b	Chong D, Rebeyrol C, José R, Williams A, Brown J, Scotton C, et al. ICAM-1 and ICAM-2 Are Differentially Expressed and Up-Regulated on Inflamed Pulmonary Epithelium, but Neither ICAM-2 nor LFA-1: ICAM-1 Are Required for Neutrophil Migration Into the Airways In Vivo. Front Immunol. 2021;12:691957 pubmed publisher
immunohistochemistry; mouse; 1:200; fig 5b western blot; human; 1:1000; fig 6g	Roth Flach R, Skoura A, Matevossian A, Danai L, Zheng W, Cortes C, et al. Endothelial protein kinase MAP4K4 promotes vascular inflammation and atherosclerosis. Nat Commun. 2015;6:8995 pubmed publisher
immunocytochemistry; human	Onken M, Li J, Cooper J. Uveal melanoma cells utilize a novel route for transendothelial migration. PLoS ONE. 2014;9:e115472 pubmed publisher
blocking or activating experiments; human; fig 6	Poussin C, Gallitz I, Schlage W, Steffen Y, Stolle K, Lebrun S, et al. Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis. Toxicol In Vitro. 2014;28:896-908 pubmed publisher
western blot; human	Lin Y, Huang C, Chen Y, Shih C, Tsao N, Lin C, et al. GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function. PLoS ONE. 2013;8:e84731 pubmed publisher
blocking or activating experiments; human	Xu W, Joo H, Clayton S, Dullaers M, Herve M, Blankenship D, et al. Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J Exp Med. 2012;209:1813-23, S1-2 pubmed
blocking or activating experiments; human	Benito Miguel M, García Carmona Y, Balsa A, Bautista Caro M, Arroyo Villa I, Cobo Ibáñez T, et al. IL-15 expression on RA synovial fibroblasts promotes B cell survival. PLoS ONE. 2012;7:e40620 pubmed publisher
immunohistochemistry; human	Crosby H, Lalor P, Ross E, Newsome P, Adams D. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. J Hepatol. 2009;51:734-49 pubmed publisher
immunocytochemistry; human	Ylostalo J, Bazhanov N, Prockop D. Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies. Exp Hematol. 2008;36:1390-402 pubmed publisher
western blot; human	Kang Y, Kim S, Kang J, Han S, Nam E, Kyung H, et al. LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes. Arthritis Rheum. 2007;56:1106-17 pubmed
western blot; human	Rajesh M, Mukhopadhyay P, BÃ¡tkai S, HaskÃ³ G, Liaudet L, Drel V, et al. Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption. Am J Physiol Heart Circ Physiol. 2007;293:H610-9 pubmed
flow cytometry; human	Ramachandran R, Morice A, Compton S. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts. Am J Respir Cell Mol Biol. 2006;35:133-41 pubmed
western blot; human	Lawson C, Ainsworth M, Yacoub M, Rose M. Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism. J Immunol. 1999;162:2990-6 pubmed
immunoprecipitation; human	Abe Y, Ballantyne C, Smith C. Functions of domain 1 and 4 of vascular cell adhesion molecule-1 in alpha4 integrin-dependent adhesion under static and flow conditions are differentially regulated. J Immunol. 1996;157:5061-9 pubmed
	Liu Y, Ye S, He S, Chi D, Wang X, Wen Y, et al. Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma. Nat Commun. 2024;15:7713 pubmed publisher
	Suehiro J, Kimura T, Fukutomi T, Naito H, Kanki Y, Wada Y, et al. Endothelial cell-specific LAT1 ablation normalizes tumor vasculature. JCI Insight. 2024;9: pubmed publisher
	Park C, Yoshihara H, Gao Q, Qu C, Iacobucci I, Ghate P, et al. Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia. Cell Rep. 2023;42:112804 pubmed publisher
	Constant O, Maarifi G, Barthelemy J, Martin M, Tinto B, Savini G, et al. Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood-brain barrier integrity. Emerg Microbes Infect. 2023;12:2156815 pubmed publisher
	Blatchley M, Hall F, Ntekoumes D, Cho H, Kailash V, Vazquez Duhalt R, et al. Discretizing Three-Dimensional Oxygen Gradients to Modulate and Investigate Cellular Processes. Adv Sci (Weinh). 2021;8:e2100190 pubmed publisher
	Takeuchi M, Vidigal P, Guerra M, Hundt M, Robert M, Olave Martinez M, et al. Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitis. Gut. 2021;70:342-356 pubmed publisher
	Clé M, Desmetz C, Barthelemy J, Martin M, Constant O, Maarifi G, et al. Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier. MBio. 2020;11: pubmed publisher
	Schacher N, Raaz Schrauder D, Pasutto F, Stumpfe F, Tauchi M, Dietel B, et al. Impact of single nucleotide polymorphisms in the VEGFR2 gene on endothelial cell activation under non‑uniform shear stress. Int J Mol Med. 2019;44:1366-1376 pubmed publisher
	Yousef H, Czupalla C, Lee D, Chen M, Burke A, Zera K, et al. Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nat Med. 2019;25:988-1000 pubmed publisher
	Fearnley G, Abdul Zani I, Latham A, Hollstein M, Ladbury J, Wheatcroft S, et al. Tpl2 is required for VEGF-A-stimulated signal transduction and endothelial cell function. Biol Open. 2019;8: pubmed publisher
	Koura S, Salama M, El Hussiny M, Khalil M, Lotfy A, Hassan S, et al. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell‑derived osteoprogenitor cells independent of serotonin concentration. Mol Med Rep. 2019;19:2611-2619 pubmed publisher
	Li A, Cho J, Reid B, Tseng C, He L, Tan P, et al. Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks. Nat Commun. 2018;9:5377 pubmed publisher
	Luz N, Khouri R, Van Weyenbergh J, Zanette D, Fiuza P, Noronha A, et al. Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression. Front Microbiol. 2018;9:2283 pubmed publisher
	Meng J, Zhou C, Hu B, Luo M, Yang Y, Wang Y, et al. Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation. Front Pharmacol. 2018;9:1053 pubmed publisher
	Byrnes L, Wong D, Subramaniam M, Meyer N, Gilchrist C, Knox S, et al. Lineage dynamics of murine pancreatic development at single-cell resolution. Nat Commun. 2018;9:3922 pubmed publisher
	Lager A, Corradin O, Cregg J, Elitt M, Shick H, Clayton B, et al. Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells. Nat Commun. 2018;9:3708 pubmed publisher
	Hofmann P, Sommer J, Theodorou K, Kirchhof L, Fischer A, Li Y, et al. Long non-coding RNA H19 regulates endothelial cell aging via inhibition of STAT3 signalling. Cardiovasc Res. 2019;115:230-242 pubmed publisher
	Kotha S, Hayes B, Phong K, Redd M, Bomsztyk K, Ramakrishnan A, et al. Engineering a multicellular vascular niche to model hematopoietic cell trafficking. Stem Cell Res Ther. 2018;9:77 pubmed publisher
	Lam F, Da Q, Guillory B, Cruz M. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium. J Immunol. 2018;200:1718-1726 pubmed publisher
	Stewart Hutchinson P, Szasz T, Jaeger E, Onken M, Cooper J, Morley S. Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate. J Leukoc Biol. 2017;102:941-948 pubmed publisher
	Aldape M, Tao A, Heeney D, McIndoo E, French J, Xu D. Experimental identification and computational characterization of a novel extracellular metalloproteinase produced by Clostridium sordellii. RSC Adv. 2017;7:13928-13938 pubmed publisher
	Wadkin J, Patten D, Kamarajah S, Shepherd E, Novitskaya V, Berditchevski F, et al. CD151 supports VCAM-1-mediated lymphocyte adhesion to liver endothelium and is upregulated in chronic liver disease and hepatocellular carcinoma. Am J Physiol Gastrointest Liver Physiol. 2017;313:G138-G149 pubmed publisher
	Hegde M, Mukherjee M, Grada Z, Pignata A, Landi D, Navai S, et al. Tandem CAR T cells targeting HER2 and IL13R?2 mitigate tumor antigen escape. J Clin Invest. 2016;126:3036-52 pubmed publisher
	Inoue Mochita M, Inoue T, Fujimoto T, Kameda T, Awai Kasaoka N, Ohtsu N, et al. p38 MAP kinase inhibitor suppresses transforming growth factor-Î²2-induced type 1 collagen production in trabecular meshwork cells. PLoS ONE. 2015;10:e0120774 pubmed publisher
	Roe K, Orillo B, Verma S. West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model. PLoS ONE. 2014;9:e102598 pubmed publisher
	Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, et al. Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece. PLoS ONE. 2014;9:e94860 pubmed publisher
	Park S, Kim K, Kim W, Cho C. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor. J Immunol. 2014;192:3003-10 pubmed publisher
	Lauridsen H, Pober J, Gonzalez A. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment. FASEB J. 2014;28:1166-80 pubmed publisher
	Ridgway L, Wetzel M, Ngo J, Erdreich Epstein A, Marchetti D. Heparanase-induced GEF-H1 signaling regulates the cytoskeletal dynamics of brain metastatic breast cancer cells. Mol Cancer Res. 2012;10:689-702 pubmed publisher
	Loginov R, Halme L, Arola J, Hockerstedt K, Lautenschlager I. Intragraft immunological events associated with EBV DNAemia in liver transplant patients. APMIS. 2010;118:888-94 pubmed publisher
	Zhao Y, Howatt D, Gizard F, Nomiyama T, Findeisen H, Heywood E, et al. Deficiency of the NR4A orphan nuclear receptor NOR1 decreases monocyte adhesion and atherosclerosis. Circ Res. 2010;107:501-11 pubmed publisher
	Smedlund K, Vazquez G. Involvement of native TRPC3 proteins in ATP-dependent expression of VCAM-1 and monocyte adherence in coronary artery endothelial cells. Arterioscler Thromb Vasc Biol. 2008;28:2049-55 pubmed publisher
	Goval J, Thielen C, Bourguignon C, Greimers R, Dejardin E, Choi Y, et al. The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP. Haematologica. 2008;93:1169-77 pubmed publisher
	Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou J, Charreau B. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood. 2007;109:2806-14 pubmed
	Kelly K, Allport J, Yu A, Sinh S, Sage E, Gerszten R, et al. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration. J Leukoc Biol. 2007;81:748-56 pubmed
	Wang J, Mahmud S, Nguyen J, Slungaard A. Thiocyanate-dependent induction of endothelial cell adhesion molecule expression by phagocyte peroxidases: a novel HOSCN-specific oxidant mechanism to amplify inflammation. J Immunol. 2006;177:8714-22 pubmed
	Fitau J, Boulday G, Coulon F, Quillard T, Charreau B. The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways. J Biol Chem. 2006;281:20148-59 pubmed
	Piantelli M, Rossi C, Iezzi M, La Sorda R, Iacobelli S, Alberti S, et al. Flavonoids inhibit melanoma lung metastasis by impairing tumor cells endothelium interactions. J Cell Physiol. 2006;207:23-9 pubmed
	Yamamoto H, Nagata M, Sakamoto Y. CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Ann Allergy Asthma Immunol. 2005;94:292-300 pubmed

product information

master code :

BBA5

SKU :

BBA5

product name :

Human VCAM-1/CD106 Antibody

unit size :

100 ug

description :

The Human VCAM-1/CD106 Antibody from R&D Systems is a mouse monoclonal antibody to VCAM-1/CD106. This antibody reacts with human,rat,insect - aedes albopictus (asian tiger mosquito). The Human VCAM-1/CD106 Antibody has been validated for the following applications: Flow Cytometry,Immunocytochemistry,Neutralization,Western Blot,Blocking,ELISA Development,ELISA Development (Capture),Immunoprecipitation,Functional Assay,Immunohistochemistry,Adhesion Blockade.

target :

VCAM-1/CD106

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose.

clonality :

Monoclonal

clone :

BBIG-V1

concentration :

LYOPH

conjugate :

Unconjugated

host :

Mouse

immunogen :

Activated HUVEC human umbilical vein endothelial cells

isotype :

IgG1

purity :

Protein A or G purified from hybridoma culture supernatant

species :

Human,Rat,Insect - Aedes albopictus (Asian tiger mosquito)

specificity :

The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.

gene symbol :

VCAM1

endotoxin note :

<0.10 EU per 1 ╡g of the antibody by the LAL method.

top caption :

VCAM-1/CD106 antibody in HUVEC Cells by Immunocytochemistry (ICC).

applications :

Flow Cytometry,Immunocytochemistry,Neutralization,Western Blot,Blocking,ELISA Development,ELISA Development (Capture),Immunoprecipitation,Functional Assay,Immunohistochemistry,Adhesion Blockade

USD :

439 USD

alt names :

CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage