Human IL-12 Biotinylated Antibody | R&D Systems BAF219 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human IL-12 Biotinylated Antibody

catalog :

BAF219

quantity :

100 ug

price :

619 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

biotin

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

more info or order :

R&D Systems product webpage

citations: 4

Reference
Nirschl C, Brodkin H, Domonkos C, Dwyer C, Hicklin D, Ismail N, et al. mWTX-330, an IL-12 INDUKINE Molecule, Activates and Reshapes Tumor-Infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity. Cancer Immunol Res. 2023;11:962-977 pubmed publisher
Jones D, Nardozzi J, Sackton K, Ahmad G, Christensen E, Ringgaard L, et al. Cell surface-tethered IL-12 repolarizes the tumor immune microenvironment to enhance the efficacy of adoptive T cell therapy. Sci Adv. 2022;8:eabi8075 pubmed publisher
Georgy J, Arlt Y, Moll J, Ouzin M, Weitz H, Gremer L, et al. Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rβ1 and subsequent signal transduction. J Biol Chem. 2021;297:101295 pubmed publisher
Roberts K, Cubitt M, Carlton T, Rodrigues Duarte L, Maggiore L, Chai R, et al. Preclinical development of a bispecific TNFα/IL-23 neutralising domain antibody as a novel oral treatment for inflammatory bowel disease. Sci Rep. 2021;11:19422 pubmed publisher

product information

brand :

R&D Systems

master code :

BAF219

SKU :

BAF219

product name :

Human IL-12 Biotinylated Antibody

unit size :

100 ug

seo description :

The Human IL-12 Biotinylated Antibody from R&D Systems is a goat polyclonal antibody to IL-12. This antibody reacts with human,mouse. The Human IL-12 Biotinylated Antibody has been validated for the following applications: Array Development,ELISA Development,Luminex Development,Western Blot,ELISA Detection,ELISA Detection (Matched Antibody Pair),Immunocytochemistry.

target :

IL-12

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Biotin

dilution :

Western Blot 0.1 ug/mL, Immunocytochemistry 5-15 ug/mL, ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL, ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL

host :

Goat

immunogen :

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12 heterodimer, Arg23-Ser219 of p35; Ile23-Ser328 of p40, Accession # P29459 (p35) & P29460 (p40)

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse

specificity :

Detects human IL-12 in ELISAs and Western blots. When used in combination with the monoclonal capture antibody anti-human IL-12 p40 (Catalog # MAB609) in sandwich ELISAs, less than 2.5% cross-reactivity was observed with rhIL-12 p70. When used in combination with the monoclonal capture antibody anti-human IL-12 p70 (Catalog # MAB611) in sandwich ELISAs, less than 0.1% cross-reactivity was observed with rhIL-12 p40, rhIL-12 p35 and rmIL-12.

gene symbol :

IL12A

top caption :

IL-12 antibody in Human PBMCs by Immunocytochemistry (ICC).

accessionNumbers :

P29459 (p35) P29460 (p40)

applications :

ELISA Detection (Matched Antibody Pair),Immunocytochemistry,Array Development,ELISA Development,Luminex Development,Western Blot,ELISA Detection

USD :

619 USD

alt names :

CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12, IL-12 subunit p35, IL-12, subunit p35, IL12A, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage