product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF
catalog :
AVI7700-050
quantity :
50 ug
price :
658 USD
more info or order :
R&D Systems product webpage
image
image 1 :
R&D Systems AVI7700-050 image 1
Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein Binding Activity
product information
master code :
AVI7700
SKU :
AVI7700-050
product name :
Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF
unit size :
50 ug
description :
The Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF from R&D Systems is derived from HEK293. The Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF has been validated for the following applications: Bioactivity.
target :
IL-4R alpha
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose.
conjugate :
Biotin
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
73-82 kDa, under reducing conditions
theoretical molecular weight :
52 kDa
details of functionality :
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al . (1989) J. Cell Physiol. 140 :323. The ED50 for this effect is 5-30 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL-4 (Catalog # 204-IL ). Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human IL-4R alpha Fc Chimera Avi-tag (Catalog # AVI7700) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004 ), the concentration of Recombinant Human IL-4 (Catalog # 204-IL ) that produces 50% of the optimal binding response is approximately 3-20 ng/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein Binding Activity
accessionNumbers :
P24394.1
applications :
Bioactivity
source long :
Human embryonic kidney cell, HEK293-derived human IL-4R alpha protein Human IL-4R alpha (Gly24-His232) Accession # P24394.1 IEGRMD Human IgG1 (Pro100-Lys330) Avi-tag
source short :
HEK293
extended description :
Biotinylated
USD :
658 USD
product details :
Biotinylated
alt names :
CD124, CD124 antigen, IL-4 R alpha, IL-4 receptor subunit alpha, IL4R, IL4R alpha, IL-4R subunit alpha, IL-4RA, IL4RACD124, IL-4R-alpha, interleukin 4 receptor, interleukin-4 receptor alpha chain, interleukin-4 receptor subunit alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.