Recombinant Human 5'-Nucleotidase/CD73 His-tag Avi-tag, CF | R&D Systems AVI5795-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human 5'-Nucleotidase/CD73 His-tag Avi-tag, CF

catalog :

AVI5795-025

quantity :

25 ug

price :

588 USD

more info or order :

R&D Systems product webpage

citations: 1

Reference
Muñóz Godínez R, de Lourdes Mora García M, Weiss Steider B, Montesinos Montesinos J, Del Carmen Aguilar Lemarroy A, García Rocha R, et al. Detection of CD39 and a Highly Glycosylated Isoform of Soluble CD73 in the Plasma of Patients with Cervical Cancer: Correlation with Disease Progression. Mediators Inflamm. 2020;2020:1678780 pubmed publisher

image

image 1 :

product information

master code :

AVI5795

SKU :

AVI5795-025

product name :

Recombinant Human 5'-Nucleotidase/CD73 His-tag Avi-tag, CF

unit size :

25 ug

description :

The Recombinant Human 5'-Nucleotidase/CD73 His-tag Avi-tag, CF from R&D Systems is derived from CHO. The Recombinant Human 5'-Nucleotidase/CD73 His-tag Avi-tag, CF has been validated for the following applications: Enzyme Activity.

target :

5'-Nucleotidase/CD73

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl, CaCl2 and Glycerol.

conjugate :

Biotin

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

60-66 kDa, under reducing conditions.

theoretical molecular weight :

61 kDa

details of functionality :

Measured by its binding ability in a functional ELISA. When Human 5'-Nucleotidase/CD73 Antibody (Catalog # MAB5795) is coated at 1 ╡g/mL, 100 ╡L/well, Biotinylated Recombinant Human 5'-Nucleotidase/CD73 His-tag Avi-tag protein (Catalog # AVI5795) binds with an ED50 of 6-36 ng/mL. Measured by its ability to hydrolyze the 5Æ-phosphate group from the substrate adenosine-5Æ-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is 15,000 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

AAH65937.1

applications :

Enzyme Activity

source long :

Chinese Hamster Ovary cell line, CHO-derived human 5'-Nucleotidase/CD73 protein Human 5'-Nucleotidase/CD73 (Trp27 - Lys547) Accession # AAH65937.1 Avi-tag 6-His tag N-terminus C-terminus

source short :

CHO

extended description :

Biotinylated

USD :

588 USD

product details :

Biotinylated

alt names :

5' nucleotidase (CD73), 5'-NT, 5-NT, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, EC 3.1.3.5, ecto-5'-nucleotidase, eN, eNT, NT, NT55'-nucleotidase, NT5E, NTE, Purine 5-Prime-Nucleotidase

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage