product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Siglec-3/CD33 His-Tag Avi-Tag Protein, CF
catalog :
AVI1137-050
quantity :
50 ug
price :
564 USD
more info or order :
R&D Systems product webpage
image
image 1 :
R&D Systems AVI1137-050 image 1
Recombinant Human Siglec-3/CD33 His-Tag Avi-Tag Protein Binding Activity
product information
master code :
AVI1137
SKU :
AVI1137-050
product name :
Recombinant Human Siglec-3/CD33 His-Tag Avi-Tag Protein, CF
unit size :
50 ug
description :
The Recombinant Human Siglec-3/CD33 His-Tag Avi-Tag Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Siglec-3/CD33 His-Tag Avi-Tag Protein, CF has been validated for the following applications: Bioactivity.
target :
Siglec-3/CD33
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose.
conjugate :
Biotin
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
45-52 kDa, under reducing conditions
theoretical molecular weight :
29 kDa
details of functionality :
Measured by its binding ability in a functional ELISA. When Human Siglec-3/CD33 (6C5/2) Antibody (Catalog # MAB1137 ) is immobilized at 0.5 ╡g/mL (100 ╡L/well), Biotinylated Recombinant Human Siglec-3/CD33 His-tag Avi-tag (Catalog # AVI1137) binds with an ED50 of 60-360 pg/mL. Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Subramanian, S. et al . (2006) Blood 107 :2548. The ED50 for this effect is 0.05-0.4 ╡g/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human Siglec-3/CD33 His-Tag Avi-Tag Protein Binding Activity
accessionNumbers :
P20138.2
applications :
Bioactivity
source long :
Chinese Hamster Ovary cell line, CHO-derived human Siglec-3/CD33 protein Human Siglec-3/CD33 (Met17-His259) Accession # P20138.2 HHHHHH Avi-tag N-terminus C-terminus
source short :
CHO
extended description :
Biotinylated
USD :
564 USD
product details :
Biotinylated
alt names :
CD33, CD33 antigen, CD33 antigen (gp67), CD33 molecule, FLJ00391, gp67, myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, Sialic acid-binding Ig-like lectin 3, Siglec3, SIGLEC-3, SIGLEC3gp67
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.