product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human TNF-alpha Avi-tag Protein, CF
catalog :
AVI10728-025
quantity :
25 ug
price :
491 USD
more info or order :
R&D Systems product webpage
image
image 1 :
R&D Systems AVI10728-025 image 1
product information
master code :
AVI10728
SKU :
AVI10728-025
product name :
Recombinant Human TNF-alpha Avi-tag Protein, CF
unit size :
25 ug
description :
The Recombinant Human TNF-alpha Avi-tag Protein, CF from R&D Systems is derived from CHO. The Recombinant Human TNF-alpha Avi-tag Protein, CF has been validated for the following applications: Bioactivity, Gel Filtration Chromatography.
target :
TNF-alpha
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose.
conjugate :
Biotin
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
15-22 kDa, under reducing conditions
theoretical molecular weight :
19 kDa
details of functionality :
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human TNF RI/TNFRSF1A Fc Chimera (Catalog # 372-RI) is coated at 0.025 ╡g/mL (100 ╡L/well), Biotinylated Recombinant Human TNF-alpha Avi-tag binds with an ED50 of 2-12 ng/mL. Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycináD. Matthews,áN. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 20-200 pg/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
P01375.1
applications :
Bioactivity, Gel Filtration Chromatography
source long :
Chinese Hamster Ovary cell line, CHO-derived human TNF-alpha protein Avi-tag Human TNF alpha (Val77-Leu233) Accession # P01375.1 N-terminus C-terminus
source short :
CHO
extended description :
Biotinylated
USD :
491 USD
product details :
Biotinylated
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.