product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein, CF
catalog :
AVI10381-050
quantity :
50 ug
price :
599 USD
more info or order :
R&D Systems product webpage
image
image 1 :
R&D Systems AVI10381-050 image 1
Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein Binding Activity
product information
master code :
AVI10381
SKU :
AVI10381-050
product name :
Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein, CF
unit size :
50 ug
description :
The Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein, CF from R&D Systems is derived from CHO. The Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein, CF has been validated for the following applications: Bioactivity.
target :
B7-H3
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose.
conjugate :
Biotin
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
104-120 kDa, under reducing conditions
theoretical molecular weight :
75 kDa
details of functionality :
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.1-1 ╡g/mL. Measured by its binding ability in a functional ELISA. When Human B7-H3 Antibody (Catalog # AF1027 ) is immobilized at 1.0 ╡g/mL, 100 ╡L/well, the concentration of Biotinylated Recombinant B7-H3 (4Ig)/B7-H3b Fc Chimera Avi-tag (Catalog # AVI10381)that produces 50% of the optimal binding response is approximately 10-60 ng/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Avi-tag Protein Binding Activity
accessionNumbers :
Q5ZPR3.1
applications :
Bioactivity
source long :
Chinese Hamster Ovary cell line, CHO-derived human B7-H3 protein Human B7-H3 (4Ig)/B7-H3b (Leu29-Thr461) Accession # Q5ZPR3.1 IEGRMD Human IgG1 (Pro100-Lys330) Avi-tag N-terminus C-terminus
source short :
CHO
USD :
599 USD
alt names :
B7H3, B7H34Ig-B7-H3, B7-H3B7 homolog 3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.