Recombinant Human EGF, Animal-Free Protein | R&D Systems AFL236 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human EGF, Animal-Free Protein

catalog :

AFL236

quantity :

1 mg (also 200 ug)

price :

599 USD

more info or order :

R&D Systems product webpage

citations: 4

Reference
Berlak M, Tucker E, Dorel M, Winkler A, McGearey A, Rodríguez Fos E, et al. Mutations in ALK signaling pathways conferring resistance to ALK inhibitor treatment lead to collateral vulnerabilities in neuroblastoma cells. Mol Cancer. 2022;21:126 pubmed publisher
Ding S, Hsu C, Wang Z, Natesh N, Millen R, Negrete M, et al. Patient-derived micro-organospheres enable clinical precision oncology. Cell Stem Cell. 2022;29:905-917.e6 pubmed publisher
Baker M, Cooke M, Kreider Letterman G, Garcia Mata R, Janmey P, Kazanietz M. Evaluation of active Rac1 levels in cancer cells: A case of misleading conclusions from immunofluorescence analysis. J Biol Chem. 2020;295:13698-13710 pubmed publisher
Cui Y, Kim H, Lee M, Yi J, Kim R, Uddin N, et al. FBXL14 abolishes breast cancer progression by targeting CDCP1 for proteasomal degradation. Oncogene. 2018;37:5794-5809 pubmed publisher

image

image 1 :

product information

master code :

AFL236

SKU :

AFL236

product name :

Recombinant Human EGF, Animal-Free Protein

unit size :

1 mg (also 200 ug)

description :

The Recombinant Human EGF, Animal-Free Protein from R&D Systems is derived from E. coli. The Recombinant Human EGF, Animal-Free Protein has been validated for the following applications: Bioactivity.

target :

EGF

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

theoretical molecular weight :

6 kDa

gene symbol :

EGF

details of functionality :

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al . (1991) Proc. Natl. Acad. Sci. USA 88 :415. The ED50 for this effect is 20-100 pg/mL. The specific activity of Recombinant Human EGF is 8.0 x 105 IU/mg, which is calibrated against the human EGF WHO International Standard (NIBSC code: 91/530).

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P01133

applications :

Bioactivity

source long :

E. coli -derived human EGF protein Asn971-Arg1023, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.

source short :

E. coli

extended description :

Intended for preclinical researchers who may transition to GMP EGF for their clinical work

USD :

599 USD

product details :

Intended for preclinical researchers who may transition to GMP EGF for their clinical work

alt names :

beta-urogastrone, epidermal growth factor, epidermal growth factor (beta-urogastrone), hEGF, HOMG4, pro-epidermal growth factor, URG, Urogastrone

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage