product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human M-CSF, Animal-Free Protein
catalog :
AFL216
quantity :
1 mg (also 5 ug, 25 ug, 500 ug)
price :
8175 USD
more info or order :
citations: 1
Reference
Hutchinson J, Ahrens N, Geissler E. MITAP-compliant characterization of human regulatory macrophages. Transpl Int. 2017;30:765-775 pubmed publisher
product information
master code :
AFL216
SKU :
AFL216
product name :
Recombinant Human M-CSF, Animal-Free Protein
unit size :
1 mg (also 5 ug, 25 ug, 500 ug)
description :
The Recombinant Human M-CSF, Animal-Free Protein from R&D Systems is derived from E. coli. The Recombinant Human M-CSF, Animal-Free Protein has been validated for the following applications: Bioactivity.
target :
M-CSF
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
observed molecular weight :
37 kDa, non-reducing conditions
theoretical molecular weight :
18.5 kDa (monomer)
gene symbol :
CSF1
details of functionality :
Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al . (1990) J. Immunol. 145 :860. The ED50 for this effect is 0.5-1.5 ng/mL. The specific activity of Recombinant Human M-CSF is 6.0 x 107 IU/mg, which is calibrated against the human M-CSF WHO International Standard (NIBSC code: 89/512).
endotoxin note :
<0.01 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
NP_757350.2
applications :
Bioactivity
source long :
E. coli -derived human M-CSF protein Glu33-Ser190, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
source short :
E. coli
extended description :
Intended for preclinical researchers who may transition to GMP M-CSF for their clinical work
USD :
8175 USD
product details :
Intended for preclinical researchers who may transition to GMP M-CSF for their clinical work
alt names :
colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, MCSF, M-CSF, MCSFlanimostim, MGC31930
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.