product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human TNF-alpha, Animal-Free Protein
catalog :
AFL210-02M
quantity :
2 mg (also 100 ug)
price :
3057 USD
more info or order :
R&D Systems product webpage
product information
master code :
AFL210
SKU :
AFL210-02M
product name :
Recombinant Human TNF-alpha, Animal-Free Protein
unit size :
2 mg (also 100 ug)
description :
The Recombinant Human TNF-alpha, Animal-Free Protein from R&D Systems is derived from E. coli. The Recombinant Human TNF-alpha, Animal-Free Protein has been validated for the following applications: Bioactivity.
target :
TNF-alpha
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
17 kDa, reducing conditions
theoretical molecular weight :
17 kDa
gene symbol :
TNF
details of functionality :
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycináD. Matthews,áN. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL. The specific activity of recombinant human TNF-alpha is 4.3 x 107 IU/mg, which is calibrated against the human TNF-alpha WHO International Standard (NIBSC code: 12/154).
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
P01375.1
applications :
Bioactivity
source long :
E. coli -derived human TNF-alpha protein Val77-Leu233, with and without an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
source short :
E. coli
extended description :
Intended for preclinical researchers who may transition to GMP TNF-alpha for their clinical work
USD :
3057 USD
product details :
Intended for preclinical researchers who may transition to GMP TNF-alpha for their clinical work
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.