Recombinant Human Sonic Hedgehog/Shh (C24II) Animal-Free, CF | R&D Systems AFL1845-025 product information

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product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Sonic Hedgehog/Shh (C24II) Animal-Free, CF

catalog :

AFL1845-025

quantity :

25 ug (also 100 ug, 500 ug)

price :

401 USD

product information

master code :

AFL1845

SKU :

AFL1845-025

product name :

Recombinant Human Sonic Hedgehog/Shh (C24II) Animal-Free, CF

unit size :

25 ug (also 100 ug, 500 ug)

description :

The Recombinant Human Sonic Hedgehog/Shh (C24II) Animal-Free, CF from R&D Systems is derived from E. coli. The Recombinant Human Sonic Hedgehog/Shh (C24II) Animal-Free, CF has been validated for the following applications: Bioactivity.

target :

Sonic Hedgehog/Shh

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS and NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

22 kDa, reducing conditions

theoretical molecular weight :

20 kDa

details of functionality :

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al . (1997) Biochem. Biophys. Res. Commun. 237 :465. The ED50 for this effect is 0.1-0.4 ╡g/mL.

endotoxin note :

<0.01 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_000184.1

applications :

Bioactivity

source long :

E. coli -derived human Sonic Hedgehog/Shh protein Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.

source short :

E. coli

extended description :

Intended for preclinical researchers who may transition to GMP Sonic Hedgehog/Shh (C24II) for their clinical work

USD :

401 USD

product details :

Intended for preclinical researchers who may transition to GMP Sonic Hedgehog/Shh (C24II) for their clinical work

alt names :

HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.