product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse Cathepsin B Antibody
catalog :
AF965
quantity :
100 ug (also 25 ug)
price :
489 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
citations: 36
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; loading ...; fig 1a
Iwama H, Mehanna S, Imasaka M, Hashidume S, Nishiura H, Yamamura K, et al. Cathepsin B and D deficiency in the mouse pancreas induces impaired autophagy and chronic pancreatitis. Sci Rep. 2021;11:6596 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 10 ug/ml; loading ...; fig 2c, 2e
Bando Y, Tokuda N, Ogasawara Y, Onozawa G, Nagasaka A, Sakiyama K, et al. Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae. Histochem Cell Biol. 2021;155:439-449 pubmed publisher
Takahashi H, Bhagwagar S, Nies S, Ye H, Han X, Chiasseu M, et al. Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase. Nat Commun. 2024;15:1434 pubmed publisher
Do x11f ru A, Rehders M, Brix K. Investigations on Primary Cilia of Nthy-ori 3-1 Cells upon Cysteine Cathepsin Inhibition or Thyrotropin Stimulation. Int J Mol Sci. 2023;24: pubmed publisher
Zhang T, Pang W, Feng T, Guo J, Wu K, Nunez Santos M, et al. TMEM106B regulates microglial proliferation and survival in response to demyelination. Sci Adv. 2023;9:eadd2676 pubmed publisher
Hua J, Garcia de Paco E, Linck N, Maurice T, Desrumaux C, Manoury B, et al. Microglial P2X4 receptors promote ApoE degradation and contribute to memory deficits in Alzheimer's disease. Cell Mol Life Sci. 2023;80:138 pubmed publisher
Zhu X, Prakash S, McAuliffe G, Pan P. Synaptojanin1 Modifies Endolysosomal Parameters in Cultured Ventral Midbrain Neurons. Eneuro. 2023;10: pubmed publisher
Gaudioso x, Jiang X, Casas J, Schuchman E, Ledesma M. Sphingomyelin 16:0 is a therapeutic target for neuronal death in acid sphingomyelinase deficiency. Cell Death Dis. 2023;14:248 pubmed publisher
Qiu Y, Shen X, Ravid O, Atrakchi D, Rand D, Wight A, et al. Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease. Proc Natl Acad Sci U S A. 2023;120:e2218915120 pubmed publisher
Parchure A, Tian M, Stalder D, Boyer C, Bearrows S, Rohli K, et al. Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules. J Cell Biol. 2022;221: pubmed publisher
Ratto E, Chowdhury S, Siefert N, Schneider M, Wittmann M, Helm D, et al. Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly. Nat Commun. 2022;13:4848 pubmed publisher
Feng T, Luan L, Katz I, Ullah M, Van Deerlin V, Trojanowski J, et al. TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss. Acta Neuropathol Commun. 2022;10:33 pubmed publisher
Uehara K, Koyanagi Aoi M, Koide T, Itoh T, Aoi T. Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids. Stem Cell Reports. 2022;17:820-834 pubmed publisher
Sun J, Liu Y, Hao X, Lin W, Su W, Chiang E, et al. LAMTOR1 inhibition of TRPML1-dependent lysosomal calcium release regulates dendritic lysosome trafficking and hippocampal neuronal function. EMBO J. 2022;41:e108119 pubmed publisher
Dang T, Back S. Translation Inhibitors Activate Autophagy Master Regulators TFEB and TFE3. Int J Mol Sci. 2021;22: pubmed publisher
Nieto Torres J, Shanahan S, Chassefeyre R, Chaiamarit T, Zaretski S, Landeras Bueno S, et al. LC3B phosphorylation regulates FYCO1 binding and directional transport of autophagosomes. Curr Biol. 2021;31:3440-3449.e7 pubmed publisher
DU H, Wong M, Zhang T, Santos M, Hsu C, Zhang J, et al. A multifaceted role of progranulin in regulating amyloid-beta dynamics and responses. Life Sci Alliance. 2021;4: pubmed publisher
Roney J, Li S, Farfel Becker T, Huang N, Sun T, Xie Y, et al. Lipid-mediated motor-adaptor sequestration impairs axonal lysosome delivery leading to autophagic stress and dystrophy in Niemann-Pick type C. Dev Cell. 2021;56:1452-1468.e8 pubmed publisher
Bonet Ponce L, Beilina A, Williamson C, Lindberg E, Kluss J, Saez Atienzar S, et al. LRRK2 mediates tubulation and vesicle sorting from lysosomes. Sci Adv. 2020;6: pubmed publisher
Yang C, Chen X, Li Z, Wu H, Jing K, Huang X, et al. SMAD3 promotes autophagy dysregulation by triggering lysosome depletion in tubular epithelial cells in diabetic nephropathy. Autophagy. 2020;:1-20 pubmed publisher
Huang M, Modeste E, Dammer E, Merino P, Taylor G, Duong D, et al. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations. Acta Neuropathol Commun. 2020;8:163 pubmed publisher
Werner G, Damme M, Schludi M, Gn xf6 rich J, Wind K, Fellerer K, et al. Loss of TMEM106B potentiates lysosomal and FTLD-like pathology in progranulin-deficient mice. EMBO Rep. 2020;21:e50241 pubmed publisher
Zhang J, Velmeshev D, Hashimoto K, Huang Y, Hofmann J, Shi X, et al. Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency. Nature. 2020;588:459-465 pubmed publisher
Feng T, Mai S, Roscoe J, Sheng R, Ullah M, Zhang J, et al. Loss of TMEM106B and PGRN leads to severe lysosomal abnormalities and neurodegeneration in mice. EMBO Rep. 2020;21:e50219 pubmed publisher
Ziros P, Renaud C, Chartoumpekis D, Bongiovanni M, Habeos I, Liao X, et al. Mice Hypomorphic for Keap1, a Negative Regulator of the Nrf2 Antioxidant Response, Show Age-Dependent Diffuse Goiter with Elevated Thyrotropin Levels. Thyroid. 2021;31:23-35 pubmed publisher
Selkrig J, Li N, Hausmann A, Mangan M, Zietek M, Mateus A, et al. Spatiotemporal proteomics uncovers cathepsin-dependent macrophage cell death during Salmonella infection. Nat Microbiol. 2020;5:1119-1133 pubmed publisher
Cui W, Sathyanarayan A, Lopresti M, Aghajan M, Chen C, Mashek D. Lipophagy-derived fatty acids undergo extracellular efflux via lysosomal exocytosis. Autophagy. 2020;:1-16 pubmed publisher
Schwenck J, Maurer A, Fehrenbacher B, Mehling R, Knopf P, Mucha N, et al. Cysteine-type cathepsins promote the effector phase of acute cutaneous delayed-type hypersensitivity reactions. Theranostics. 2019;9:3903-3917 pubmed publisher
Farfel Becker T, Roney J, Cheng X, Li S, Cuddy S, Sheng Z. Neuronal Soma-Derived Degradative Lysosomes Are Continuously Delivered to Distal Axons to Maintain Local Degradation Capacity. Cell Rep. 2019;28:51-64.e4 pubmed publisher
Takagaki Y, Shi S, Katoh M, Kitada M, Kanasaki K, Koya D. Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice. Sci Rep. 2019;9:7519 pubmed publisher
Gabandé Rodríguez E, Pérez Cañamás A, Soto Huelin B, Mitroi D, Sánchez Redondo S, Martínez Sáez E, et al. Lipid-induced lysosomal damage after demyelination corrupts microglia protective function in lysosomal storage disorders. EMBO J. 2019;38: pubmed publisher
Götzl J, Colombo A, Fellerer K, Reifschneider A, Werner G, Tahirovic S, et al. Early lysosomal maturation deficits in microglia triggers enhanced lysosomal activity in other brain cells of progranulin knockout mice. Mol Neurodegener. 2018;13:48 pubmed publisher
Cheng X, Xie Y, Zhou B, Huang N, Farfel Becker T, Sheng Z. Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons. J Cell Biol. 2018;217:3127-3139 pubmed publisher
Palm W, Araki J, King B, Dematteo R, Thompson C. Critical role for PI3-kinase in regulating the use of proteins as an amino acid source. Proc Natl Acad Sci U S A. 2017;114:E8628-E8636 pubmed publisher
Klein Z, Takahashi H, Ma M, Stagi M, Zhou M, Lam T, et al. Loss of TMEM106B Ameliorates Lysosomal and Frontotemporal Dementia-Related Phenotypes in Progranulin-Deficient Mice. Neuron. 2017;95:281-296.e6 pubmed publisher
Takahashi K, Mashima H, Miura K, Maeda D, Goto A, Goto T, et al. Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models. Sci Rep. 2017;7:2817 pubmed publisher
product information
brand :
R&D Systems
master code :
AF965
SKU :
AF965
product name :
Mouse Cathepsin B Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Mouse Cathepsin B Antibody from R&D Systems is a goat polyclonal antibody to Cathepsin B. This antibody reacts with human,mouse,rat,transgenic mouse. The Mouse Cathepsin B Antibody has been validated for the following applications: Western Blot,Flow Cytometry,Immunohistochemistry,Immunocytochemistry,Neutralization.
target :
Cathepsin B
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.1 ug/mL, Immunohistochemistry 5-15 ug/mL, Neutralization 0.33 ug/mL
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin B, His18-Phe339, Accession # P10605
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat,Transgenic Mouse
specificity :
Detects mouse Cathepsin B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Cathepsin B is observed and less than 5% cross-reactivity with recombinant mouse Cathepsin H is observed.
gene symbol :
CTSB
top caption :
Cathepsin B antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr).
accessionNumbers :
P10605
applications :
Neutralization,Flow Cytometry,Immunohistochemistry,Western Blot,Immunocytochemistry
USD :
489 USD
alt names :
APP secretase, APPS, cathepsin B, Cathepsin B1, CPSBamyloid precursor protein secretase, CTSB, cysteine protease, EC 3.4.22, EC 3.4.22.1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.