product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human EpCAM/TROP-1 Antibody
catalog :
AF960
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, western blot knockout validation, immunohistochemistry knockout validation
more info or order :
R&D Systems product webpage
citations: 32
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; 5 ug/ml; loading ...
  • western blot knockout validation; mouse; 1 ug/ml; loading ...; fig 1a
Szabo R, Ward J, Artunc F, Bugge T. EPCAM and TROP2 share a role in claudin stabilization and development of intestinal and extraintestinal epithelia in mice. Biol Open. 2022;11: pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig 1c
Della Chiara G, Gervasoni F, Fakiola M, Godano C, D Oria C, Azzolin L, et al. Epigenomic landscape of human colorectal cancer unveils an aberrant core of pan-cancer enhancers orchestrated by YAP/TAZ. Nat Commun. 2021;12:2340 pubmed publisher
Thiyagarajan R, Taub M. Studies with Human-Induced Pluripotent Stem Cells Reveal That CTNS Mutations Can Alter Renal Proximal Tubule Differentiation. Int J Mol Sci. 2023;24: pubmed publisher
Sun N, Zhang C, Wang J, Yue X, Kim H, Zhang R, et al. Hierarchical integration of DNA nanostructures and NanoGold onto a microchip facilitates covalent chemistry-mediated purification of circulating tumor cells in head and neck squamous cell carcinoma. Nano Today. 2023;49: pubmed publisher
Hosokawa M, Mikawa R, Hagiwara A, Okuno Y, Awaya T, Yamamoto Y, et al. Cryptotanshinone is a candidate therapeutic agent for interstitial lung disease associated with a BRICHOS-domain mutation of SFTPC. iScience. 2023;26:107731 pubmed publisher
Szabo R, Kawaguchi M, Kataoka H, Bugge T. Early-onset tufting enteropathy in HAI-2-deficient mice is independent of matriptase-mediated cleavage of EpCAM. Development. 2023;150: pubmed publisher
Zhao C, Wang D, Li Z, Zhang Z, Xu Y, Liu J, et al. IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer. Int Immunopharmacol. 2023;121:110457 pubmed publisher
Matkovic Leko I, Schneider R, Thimraj T, Schrode N, Beitler D, Liu H, et al. A distal lung organoid model to study interstitial lung disease, viral infection and human lung development. Nat Protoc. 2023;18:2283-2312 pubmed publisher
Sperger J, Helzer K, Stahlfeld C, Jiang D, Singh A, Kaufmann K, et al. Expression and Therapeutic Targeting of TROP-2 in Treatment-Resistant Prostate Cancer. Clin Cancer Res. 2023;29:2324-2335 pubmed publisher
Leko I, Schrode N, Torres J, Pezet M, Thimraj T, BEAUMONT K, et al. Generation and expansion of transitional lung organoids from human pluripotent stem cells. bioRxiv. 2023;: pubmed publisher
Wang J, Sun N, Lee Y, Kim M, Vagner T, Rohena Rivera K, et al. Prostate cancer extracellular vesicle digital scoring assay - a rapid noninvasive approach for quantification of disease-relevant mRNAs. Nano Today. 2023;48: pubmed publisher
Chen M, Assis D, Benet M, McClung C, Gordon E, Ghose S, et al. Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer. Commun Biol. 2023;6:48 pubmed publisher
Rodems T, Heninger E, Stahlfeld C, Gilsdorf C, Carlson K, Kircher M, et al. Reversible epigenetic alterations regulate class I HLA loss in prostate cancer. Commun Biol. 2022;5:897 pubmed publisher
Peterson H, Chin L, Iwamoto Y, Oh J, Carlson J, Lee H, et al. Integrated Analytical System for Clinical Single-Cell Analysis. Adv Sci (Weinh). 2022;9:e2200415 pubmed publisher
Yang H, Deng Q, Ni T, Liu Y, Lu L, Dai H, et al. Targeted Inhibition of LPL/FABP4/CPT1 fatty acid metabolic axis can effectively prevent the progression of nonalcoholic steatohepatitis to liver cancer. Int J Biol Sci. 2021;17:4207-4222 pubmed publisher
Zhang J, Nguyen L, Hickey R, Walters N, Wang X, Kwak K, et al. Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids. Sci Rep. 2021;11:8034 pubmed publisher
Chan K, Gleadle J, Li J, Michl T, Vasilev K, Macgregor M. Improving hexaminolevulinate enabled cancer cell detection in liquid biopsy immunosensors. Sci Rep. 2021;11:7283 pubmed publisher
Heyward C, Dong L, Shakhzadyan H, Wan C, Stokol T. Detection of Epithelial Cell Adhesion Molecule in Feline Normal and Tumor Cell Lines and Tissues With Selected Commercial Anti-human EpCAM Antibodies. Front Vet Sci. 2021;8:622189 pubmed publisher
Bade R, Schehr J, Emamekhoo H, Gibbs B, Rodems T, Mannino M, et al. Development and initial clinical testing of a multiplexed circulating tumor cell assay in patients with clear cell renal cell carcinoma. Mol Oncol. 2021;15:2330-2344 pubmed publisher
Koike H, Iwasawa K, Ouchi R, Maezawa M, Kimura M, Kodaka A, et al. Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells. Nat Protoc. 2021;16:919-936 pubmed publisher
Hor P, Ichida J, Borok Z, Ryan A. Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells. STAR Protoc. 2020;1:100068 pubmed publisher
Lin L, Kost E, Lin C, Valente P, Wang C, Kolonin M, et al. PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer. Cell Rep. 2020;33:108253 pubmed publisher
Sun N, Lee Y, Zhang R, Kao R, Teng P, Yang Y, et al. Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring. Nat Commun. 2020;11:4489 pubmed publisher
Dias A, Lozovska A, Wymeersch F, Novoa A, Binagui Casas A, Sobral D, et al. A Tgfbr1/Snai1-dependent developmental module at the core of vertebrate axial elongation. elife. 2020;9: pubmed publisher
Morral C, Stanisavljevic J, Hernando Momblona X, Mereu E, Álvarez Varela A, Cortina C, et al. Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer. Cell Stem Cell. 2020;26:845-861.e12 pubmed publisher
Singh A, Denu R, Wolfe S, Sperger J, Schehr J, Witkowsky T, et al. Centrosome amplification is a frequent event in circulating tumor cells from subjects with metastatic breast cancer. Mol Oncol. 2020;: pubmed publisher
Yoshida S, Miwa H, Kawachi T, Kume S, Takahashi K. Generation of intestinal organoids derived from human pluripotent stem cells for drug testing. Sci Rep. 2020;10:5989 pubmed publisher
Tian L, Ye Z, Kafka K, Stewart D, Anders R, Schwarz K, et al. Biliary Atresia Relevant Human Induced Pluripotent Stem Cells Recapitulate Key Disease Features in a Dish. J Pediatr Gastroenterol Nutr. 2019;68:56-63 pubmed publisher
Garnier D, Li R, Delbos F, Fourrier A, Collet C, Guguen Guillouzo C, et al. Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system. Sci Rep. 2018;8:8222 pubmed publisher
Ueki T, Yoshihara A, Teramura Y, Takai M. Fast and selective cell isolation from blood sample by microfiber fabric system with vacuum aspiration. Sci Technol Adv Mater. 2016;17:807-815 pubmed
Day K, Lorenzatti Hiles G, Kozminsky M, Dawsey S, Paul A, Broses L, et al. HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone. Cancer Res. 2017;77:74-85 pubmed publisher
Hsu Y, Osmulski P, Wang Y, Huang Y, Liu L, Ruan J, et al. EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells That Promote Epithelial-to-Mesenchymal Transition. Cancer Res. 2016;76:6171-6182 pubmed
product information
master code :
AF960
SKU :
AF960
product name :
Human EpCAM/TROP-1 Antibody
unit size :
100 ug (also 25 ug)
description :
The Human EpCAM/TROP-1 Antibody from R&D Systems is a goat polyclonal antibody to EpCAM/TROP1. This antibody reacts with human,mouse. The Human EpCAM/TROP-1 Antibody has been validated for the following applications: Flow Cytometry,Immunohistochemistry,Adhesion Assay,Bioassay,Capture Assay,CTC,CyTof,Cell Selection,ELISA Capture,Immunocytochemistry,Western Blot,Simple Western,CyTOF-ready.
target :
EpCAM/TROP1
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1, extracellular domain, Gln24-Lys265, Accession # CAA32870
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse
specificity :
Detects human EpCAM/TROP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TROP-2 and approximately 26% cross-reactivity with recombinant mouse EpCAM/TROP-1 is observed.
gene symbol :
EPCAM
top caption :
Detection of Human EpCAM/TROP-1 antibody by Western Blot.
accessionNumbers :
CAA32870
applications :
Immunohistochemistry,Adhesion Assay,Bioassay,Capture Assay,CTC,CyTof,Flow Cytometry,Cell Selection,ELISA Capture,Immunocytochemistry,Western Blot,Simple Western,CyTOF-ready
USD :
499 USD
alt names :
17-1A, 323/A3, ACSTD1, antigen identified by monoclonal AUA1, CD326, CD326 antigen, Cell surface glycoprotein Trop-1, chromosome 4, surface marker (35kD glycoprotein), DIAR5, EGP, EGP-2, EGP314, EGP40, EpCAM, epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733-2, GA733-2EGP34, gp40, hEGP314, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1, M4S1Ly74, Major gastrointestinal tumor-associated protein GA733-2, MIC18MH99, MOC31, TACST-1, TACSTD1, TROP1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.