Human Cathepsin B Antibody | R&D Systems AF953 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human Cathepsin B Antibody

catalog :

AF953

quantity :

100 ug (also 25 ug)

price :

479 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 20

Reference
Drobny A, Boros F, Balta D, Prieto Huarcaya S, Caylioglu D, Qazi N, et al. Reciprocal effects of alpha-synuclein aggregation and lysosomal homeostasis in synucleinopathy models. Transl Neurodegener. 2023;12:31 pubmed publisher
Ercan H, Resch U, Hsu F, Mitulovic G, Bileck A, Gerner C, et al. A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection. Cells. 2023;12: pubmed publisher
Nakamura H, Tanaka T, Ji Y, Zheng C, Afione S, Warner B, et al. Salivary gland LAMP3 mRNA expression is a possible predictive marker in the response to hydroxychloroquine in Sjögren's disease. PLoS ONE. 2023;18:e0282227 pubmed publisher
Shah H, Stankov M, Panayotova Dimitrova D, Yazdi A, Budida R, Klusmann J, et al. Autolysosomal activation combined with lysosomal destabilization efficiently targets myeloid leukemia cells for cell death. Front Oncol. 2023;13:999738 pubmed publisher
Barral D, Staiano L, Guimas Almeida C, Cutler D, Eden E, Futter C, et al. Current methods to analyze lysosome morphology, positioning, motility and function. Traffic. 2022;23:238-269 pubmed publisher
Kovalyova Y, Bak D, Gordon E, Fung C, Shuman J, Cover T, et al. An infection-induced oxidation site regulates legumain processing and tumor growth. Nat Chem Biol. 2022;18:698-705 pubmed publisher
Chen D, Zheng Q, Sun L, Ji M, Li Y, Deng H, et al. ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress. Dev Cell. 2021;56:3250-3263.e5 pubmed publisher
Tang Q, Liu M, Liu Y, Hwang R, Zhang T, Wang J. NDST3 deacetylates α-tubulin and suppresses V-ATPase assembly and lysosomal acidification. EMBO J. 2021;40:e107204 pubmed publisher
Kern U, Fröhlich K, Bedacht J, Schmidt N, Biniossek M, Gensch N, et al. Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells. Cells. 2021;10: pubmed publisher
Cuddy L, Mazzulli J. Analysis of lysosomal hydrolase trafficking and activity in human iPSC-derived neuronal models. STAR Protoc. 2021;2:100340 pubmed publisher
Miao G, Zhao H, Li Y, Ji M, Chen Y, Shi Y, et al. ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation. Dev Cell. 2021;56:427-442.e5 pubmed publisher
Oelschlaegel D, Weiss Sadan T, Salpeter S, Krug S, Blum G, Schmitz W, et al. Cathepsin Inhibition Modulates Metabolism and Polarization of Tumor-Associated Macrophages. Cancers (Basel). 2020;12: pubmed publisher
Gomez Auli A, Hillebrand L, Christen D, G xfc nther S, Biniossek M, Peters C, et al. The secreted inhibitor of invasive cell growth CREG1 is negatively regulated by cathepsin proteases. Cell Mol Life Sci. 2021;78:733-755 pubmed publisher
Kakimoto Y, Sasaki A, Niioka M, Kawabe N, Osawa M. Myocardial cathepsin D is downregulated in sudden cardiac death. PLoS ONE. 2020;15:e0230375 pubmed publisher
Goren L, Zhang G, Kaushik S, Breslin P, Du Y, Foster D. (-)-Oleocanthal and (-)-oleocanthal-rich olive oils induce lysosomal membrane permeabilization in cancer cells. PLoS ONE. 2019;14:e0216024 pubmed publisher
Xu Y, Ren J, He X, Chen H, Wei T, Feng W. YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization. Autophagy. 2019;15:1017-1030 pubmed publisher
Tran A, Sundar S, Yu M, Lang B, Silver J. Modulation of Receptor Protein Tyrosine Phosphatase Sigma Increases Chondroitin Sulfate Proteoglycan Degradation through Cathepsin B Secretion to Enhance Axon Outgrowth. J Neurosci. 2018;38:5399-5414 pubmed publisher
Lee C, Stankowski J, Chew J, Cook C, Lam Y, Almeida S, et al. The lysosomal protein cathepsin L is a progranulin protease. Mol Neurodegener. 2017;12:55 pubmed publisher
Bailleul G, Kravtzoff A, Joulin Giet A, Lecaille F, Labas V, Meudal H, et al. The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity. PLoS ONE. 2016;11:e0161573 pubmed publisher
Huang C, Lee C, Lin H, Chang J. Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors. Sci Rep. 2016;6:29256 pubmed publisher

product information

master code :

AF953

SKU :

AF953

product name :

Human Cathepsin B Antibody

unit size :

100 ug (also 25 ug)

description :

The Human Cathepsin B Antibody from R&D Systems is a goat polyclonal antibody to Cathepsin B. This antibody reacts with avian - chicken,gerbil,human,mouse,rat,xenograft. The Human Cathepsin B Antibody has been validated for the following applications: Western Blot,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Immunoprecipitation,Simple Western,Knockout Validated.

target :

Cathepsin B

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant human Cathepsin B, Arg18-Ile339, Accession # P07858

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Avian - Chicken,Gerbil,Human,Mouse,Rat,Xenograft

specificity :

Detects human Cathepsin B in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse (rm) Cathepsin B is observed and less than 5% cross-reactivity with recombinant human (rh) Cathepsin C, rmCathepsin H, and rhCathepsin L is observed.

gene symbol :

CTSB

Antibody validation :

Knockout/Knockdown

top caption :

Detection of Human Cathepsin B antibody by Western Blot.

accessionNumbers :

P07858

applications :

Western Blot,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Immunoprecipitation,Simple Western,Knockout Validated

USD :

479 USD

alt names :

APP secretase, APPS, cathepsin B, Cathepsin B1, CPSBamyloid precursor protein secretase, CTSB, cysteine protease, EC 3.4.22, EC 3.4.22.1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage