Human SPARC Antibody | R&D Systems AF941 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human SPARC Antibody

catalog :

AF941

quantity :

100 ug (also 25 ug)

price :

499 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

R&D Systems product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...	Tsutsui K, Machida H, Nakagawa A, Ahn K, Morita R, Sekiguchi K, et al. Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions. Nat Commun. 2021;12:2577 pubmed publisher
	Lin L, Nayak B, Osmulski P, Wang E, Wang C, Valente P, et al. PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms. Cell Rep. 2024;43:114527 pubmed publisher
	Kazakova E, Rakina M, Sudarskikh T, Iamshchikov P, Tarasova A, Tashireva L, et al. Angiogenesis regulators S100A4, SPARC and SPP1 correlate with macrophage infiltration and are prognostic biomarkers in colon and rectal cancers. Front Oncol. 2023;13:1058337 pubmed publisher
	Jang H, Song M, Park C, Youn H, Lee Y, Cheon G, et al. Imaging of Indocyanine Green-Human Serum Albumin (ICG-HSA) Complex in Secreted Protein Acidic and Rich in Cysteine (SPARC)-Expressing Glioblastoma. Int J Mol Sci. 2023;24: pubmed publisher
	Kim K, Huang H, Parida P, He L, Marquez Palencia M, Reese T, et al. Cell Competition Shapes Metastatic Latency and Relapse. Cancer Discov. 2023;13:85-97 pubmed publisher
	Naito T, Yuge R, Kitadai Y, Takigawa H, Higashi Y, Kuwai T, et al. Mesenchymal stem cells induce tumor stroma formation and epithelial‑mesenchymal transition through SPARC expression in colorectal cancer. Oncol Rep. 2021;45: pubmed publisher
	Ewanchuk B, Arnold C, Balce D, Premnath P, Orsetti T, Warren A, et al. A non-immunological role for γ-interferon-inducible lysosomal thiol reductase (GILT) in osteoclastic bone resorption. Sci Adv. 2021;7: pubmed publisher
	Yin S, Gao Y, Xie X, Xu D, Riabov V, Du W. Accumulation of stabilin-1 positive macrophages in the early stage of gastric cancer is associated with short cumulative survival. Oncol Lett. 2020;19:2404-2412 pubmed publisher
	Park C, Jo J, Song M, Park J, Kim Y, Youn H, et al. Secreted protein acidic and rich in cysteine mediates active targeting of human serum albumin in U87MG xenograft mouse models. Theranostics. 2019;9:7447-7457 pubmed publisher
	Park J, Park J, Jun Y, Oh Y, Ryoo G, Jeong Y, et al. Expanding therapeutic utility of carfilzomib for breast cancer therapy by novel albumin-coated nanocrystal formulation. J Control Release. 2019;302:148-159 pubmed publisher
	Zhang J, Hao N, Liu W, Lu M, Sun L, Chen N, et al. In-depth proteomic analysis of tissue interstitial fluid for hepatocellular carcinoma serum biomarker discovery. Br J Cancer. 2017;117:1676-1684 pubmed publisher
	Riabov V, Yin S, Song B, Avdic A, Schledzewski K, Ovsiy I, et al. Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model. Oncotarget. 2016;7:31097-110 pubmed publisher

product information

brand :

R&D Systems

master code :

AF941

SKU :

AF941

product name :

Human SPARC Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human SPARC Antibody from R&D Systems is a goat polyclonal antibody to SPARC. This antibody reacts with human,mouse. The Human SPARC Antibody has been validated for the following applications: Intracellular Staining by Flow Cytometry,Western Blot,Immunocytochemistry,ELISA Development,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunofluorescence,Immunocytochemistry/ Immunofluorescence,ELISA,Simple Western,CyTOF-ready.

target :

SPARC

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 2 ug/mL, Simple Western 20 ug/mL, ELISA, Immunohistochemistry 5-15 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant human SPARC/Osteonectin, Ala18-Ile303, Accession # P09486

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse

specificity :

Detects human SPARC/Osteonectin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse SPARC is observed, and less than 3% cross-reactivity with recombinant human SPARC L1 is observed.

gene symbol :

SPARC

top caption :

Detection of Human SPARC antibody by Western Blot.

accessionNumbers :

P09486

applications :

ELISA,Simple Western,Intracellular Staining by Flow Cytometry,CyTOF-ready,ELISA Development,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunofluorescence,Immunocytochemistry,Immunocytochemistry/ Immunofluorescence

USD :

499 USD

alt names :

Basement-membrane protein 40, BM-40, ONcysteine-rich protein, osteonectin, Secreted protein acidic and rich in cysteine, secreted protein, acidic, cysteine-rich (osteonectin)

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage