product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody
catalog :
AF887
quantity :
100 ug (also 25 ug)
price :
419 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
R&D Systems product webpage
citations: 29
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; rat; 1:400; loading ...; fig s3c
  • western blot; rat; loading ...; fig s3a
Li H, Yang Q, Wang W, Tian X, Feng F, Zhang S, et al. Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3. J Neuroinflammation. 2021;18:150 pubmed publisher
Meah A, Vedarethinam V, Bronstein R, Gujarati N, Jain T, Mallipattu S, et al. Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers. Biosensors (Basel). 2023;13: pubmed publisher
Zhang F, Xie X, Chen M, Tong J, Chen Q, Feng J, et al. Poly(A)-specific ribonuclease protein promotes the proliferation, invasion and migration of esophageal cancer cells. World J Gastroenterol. 2023;29:4783-4796 pubmed publisher
Festa J, Hussain A, Hackney A, Desai U, Sahota T, Singh H, et al. Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis. Food Sci Nutr. 2023;11:4047-4059 pubmed publisher
Tarawneh N, Hamadneh L, Abu Irmaileh B, Shraideh Z, Bustanji Y, Abdalla S. Berberine Inhibited Growth and Migration of Human Colon Cancer Cell Lines by Increasing Phosphatase and Tensin and Inhibiting Aquaporins 1, 3 and 5 Expressions. Molecules. 2023;28: pubmed publisher
Nag S, Rahaman S, Guha A, Ray P. An AKT1-and TRIM21-mediated phosphodegron controls proteasomal degradation of HuR enabling cell survival under heat shock. iScience. 2023;26:106307 pubmed publisher
Mohamed O, Abdallah D, Fayez A, Mohamed R, El Abhar H. Morin post-treatment surpassed calpeptin in ameliorating 3-NP-induced cortical neurotoxicity via modulation of glutamate/calpain axis, Kidins220, and BDNF/TrkB/AKT/CREB trajectory. Int Immunopharmacol. 2023;116:109771 pubmed publisher
Teran O, Zanotelli M, Lin M, Cerione R, Wilson K. Dock7 regulates AKT and mTOR/S6K activity required for the transformed phenotypes and survival of cancer cells. bioRxiv. 2023;: pubmed publisher
Al Rawashde F, Al Wajeeh A, Vishkaei M, Saad H, Johan M, Taib W, et al. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells. Pharmaceuticals (Basel). 2022;15: pubmed publisher
Zhou M, Zhou S, Han K, Zhang J, Chen Q, Tian C, et al. Th1 immune maturation effects of Nocardia rubra cell-wall skeleton via PI3K/Akt/PAX8 regulatory axis. Sci Prog. 2022;105:368504221092901 pubmed publisher
Hwang J, Kang S, Jung H. Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway. Mol Med Rep. 2022;25: pubmed publisher
Mortoglou M, Manić L, Buha Djordjevic A, Bulat Z, Đorđević V, Manis K, et al. Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs. Toxics. 2022;10: pubmed publisher
Mortoglou M, Buha Djordjevic A, Djordjevic V, Collins H, York L, Mani K, et al. Role of microRNAs in response to cadmium chloride in pancreatic ductal adenocarcinoma. Arch Toxicol. 2022;96:467-485 pubmed publisher
Belluti S, Semeghini V, Rigillo G, Ronzio M, Benati D, Torricelli F, et al. Alternative splicing of NF-YA promotes prostate cancer aggressiveness and represents a new molecular marker for clinical stratification of patients. J Exp Clin Cancer Res. 2021;40:362 pubmed publisher
Guerenne Del Ben T, Couderc V, Duponchel L, Sol V, Leproux P, Petit J. Multiplex coherent anti-Stokes Raman scattering microspectroscopy detection of lipid droplets in cancer cells expressing TrkB. Sci Rep. 2020;10:16749 pubmed publisher
Tu G, Dai C, Qu H, Wang Y, Liao B. Role of exercise and rapamycin on the expression of energy metabolism genes in liver tissues of rats fed a high‑fat diet. Mol Med Rep. 2020;22:2932-2940 pubmed publisher
Ferreira Mendes J, de Faro Valverde L, Torres Andion Vidal M, Paredes B, Coelho P, Allahdadi K, et al. Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma. Int J Mol Sci. 2020;21: pubmed publisher
Sun X, Li Z, Zhang Y, Zhong X, He Z, Zhou J, et al. Theophylline and dexamethasone in combination reduce inflammation and prevent the decrease in HDAC2 expression seen in monocytes exposed to cigarette smoke extract. Exp Ther Med. 2020;19:3425-3431 pubmed publisher
Chen H, Fu X, Jiang J, Han S. C16 Peptide Promotes Vascular Growth and Reduces Inflammation in a Neuromyelitis Optica Model. Front Pharmacol. 2019;10:1373 pubmed publisher
Yang G, Li Z, Mei H, Ye W, Huang S, Liu K, et al. Bupivacaine at clinically relevant concentrations induces toxicity in human intervertebral disc cells via the induction of autophagy in vitro. Mol Med Rep. 2019;20:837-843 pubmed publisher
Huang H, Salavaggione O, Rivera L, Mukherjee S, Brekken R, Tennant B, et al. Woodchuck VEGF (wVEGF) characteristics: Model for angiogenesis and human hepatocellular carcinoma directed therapies. Arch Biochem Biophys. 2019;661:97-106 pubmed publisher
Kim J, Jeong J, Lee K, Kim S, Choe Y, Kwon S, et al. Phosphoinositide 3-kinase-delta could be a biomarker for eosinophilic nasal polyps. Sci Rep. 2018;8:15990 pubmed publisher
Xiao J, Egger M, McMasters K, Hao H. Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines. BMC Cancer. 2018;18:675 pubmed publisher
Agod Z, Pazmandi K, Bencze D, Vereb G, Biro T, Szabo A, et al. Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic Cells via Stabilization of Interferon Regulatory Factor 8. Front Immunol. 2018;9:62 pubmed publisher
Belloni D, Heltai S, Ponzoni M, Villa A, Vergani B, Pecciarini L, et al. Modeling multiple myeloma-bone marrow interactions and response to drugs in a 3D surrogate microenvironment. Haematologica. 2018;103:707-716 pubmed publisher
Fischer D, Kemp C, Cole Strauss A, Polinski N, Paumier K, Lipton J, et al. Subthalamic Nucleus Deep Brain Stimulation Employs trkB Signaling for Neuroprotection and Functional Restoration. J Neurosci. 2017;37:6786-6796 pubmed publisher
Budai M, Tozser J, Benko S. Different dynamics of NLRP3 inflammasome-mediated IL?1? production in GM-CSF- and M-CSF-differentiated human macrophages. J Leukoc Biol. 2017;101:1335-1347 pubmed publisher
Werling D, Reglodi D, Banks W, Salameh T, Kovacs K, Kvarik T, et al. Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents. Invest Ophthalmol Vis Sci. 2016;57:6683-6691 pubmed publisher
Divakar S, Ramana Reddy M, Cosenza S, Baker S, Perumal D, Antonelli A, et al. Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas. Leukemia. 2016;30:86-93 pubmed publisher
product information
brand :
R&D Systems
master code :
AF887
SKU :
AF887
product name :
Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody from R&D Systems is a rabbit polyclonal antibody to AKT. This antibody reacts with human,mouse,n/a,primate,rat. The Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody has been validated for the following applications: Western Blot,Bioassay,ELISA Development,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Intracellular Staining by Flow Cytometry,CyTOF-ready.
target :
Akt
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.5 ug/mL, Simple Western 5 ug/mL, Immunohistochemistry 5-15 ug/mL, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, CyTOF-ready
host :
Rabbit
immunogen :
Phosphopeptide containing human, mouse, and rat Akt1 S473 site
isotype :
IgG
purity :
Antigen and protein A Affinity-purified
species :
Human,Mouse,N/A,Primate,Rat
specificity :
Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
gene symbol :
AKT1
top caption :
Detection of Mouse and Human Phospho-Akt (S473) Pan Specific antibody by Western Blot.
applications :
Simple Western,Intracellular Staining by Flow Cytometry,CyTOF-ready,Bioassay,ELISA Development,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen
USD :
419 USD
alt names :
RAC-ALPHA, AKT, AKT serine/threonine kinase 1, PKB, PKB alpha, PKB beta, PKB gamma, PKB-ALPHA, PRKBA, RAC, RAC-alpha, RAC-beta, RAC-gamma
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.