product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse Osteopontin/OPN Antibody
catalog :
AF808
quantity :
100 ug (also 25 ug)
price :
499 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 147
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig s7b
  • immunohistochemistry; mouse; 1:20; loading ...; fig s7c
  • western blot; mouse; 1:500; loading ...; fig 4n
Shi H, Yin Z, Koronyo Y, Fuchs D, Sheyn J, Davis M, et al. Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer's-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia. Acta Neuropathol Commun. 2022;10:136 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:300; loading ...; fig s1b
Pantasis S, Friemel J, Brütsch S, Hu Z, Krautbauer S, Liebisch G, et al. Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammation. J Cell Sci. 2022;135: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 9c
Chang L, Masada M, Kojima M, Yamamoto N. Involvement of Denervated Midbrain-Derived Factors in the Formation of Ectopic Cortico-Mesencephalic Projection after Hemispherectomy. J Neurosci. 2022;42:749-761 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...
Kindt K, Akturk A, Jarysta A, Day M, Beirl A, Flonard M, et al. EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia. Nat Commun. 2021;12:2861 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 6g
Zarb Y, Sridhar S, Nassiri S, Utz S, Schaffenrath J, Maheshwari U, et al. Microglia control small vessel calcification via TREM2. Sci Adv. 2021;7: pubmed publisher
Ono K, Jarysta A, Hughes N, Jukic A, Chang H, Deans M, et al. Contributions of mirror-image hair cell orientation to mouse otolith organ and zebrafish neuromast function. bioRxiv. 2024;: pubmed publisher
Zhang Q, Xue J, Tang J, Wu S, Liu Z, Wu C, et al. Modulating amacrine cell-derived dopamine signaling promotes optic nerve regeneration and preserves visual function. Sci Adv. 2024;10:eado0866 pubmed publisher
Peruzzotti Jametti L, Willis C, Hamel R, Krzak G, Reisz J, Prag H, et al. Mitochondrial reverse electron transport in myeloid cells perpetuates neuroinflammation. bioRxiv. 2024;: pubmed publisher
Chang L, Ran Y, Yang M, Auferkorte O, Butz E, H xfc ser L, et al. Spike desensitisation as a mechanism for high-contrast selectivity in retinal ganglion cells. Front Cell Neurosci. 2023;17:1337768 pubmed publisher
Winter C, Jacobi A, Su J, Chung L, van Velthoven C, Yao Z, et al. A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons. Nature. 2023;624:403-414 pubmed publisher
Schenberg L, Palou A, Simon F, Bonnard T, Barton C, Fricker D, et al. Multisensory gaze stabilization in response to subchronic alteration of vestibular type I hair cells. elife. 2023;12: pubmed publisher
Wang Y, Zheng Y, Cong L, Wang P, Zhao L, Xing L, et al. Lymphatic platelet thrombosis limits bone repair by precluding lymphatic transporting DAMPs. Res Sq. 2023;: pubmed publisher
Kajander K, Sirki xe4 S, Vallittu P, Heino T, M xe4 xe4 tt xe4 J. Bioactive glasses promote rapid pre-osteoblastic cell migration in contrast to hydroxyapatite, while carbonated apatite shows migration inhibiting properties. Sci Rep. 2023;13:20587 pubmed publisher
Pinanga Y, Lee H, Shin E, Lee H, Pyo K, Kim J, et al. TM4SF5-mediated abnormal food-intake behavior and apelin expression facilitate non-alcoholic fatty liver disease features. iScience. 2023;26:107625 pubmed publisher
Zhao M, Toma K, Kinde B, Li L, Patel A, Wu K, et al. Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy. Cell Rep. 2023;42:113038 pubmed publisher
Tixi W, Maldonado M, Chang Y, Chiu A, Yeung W, Parveen N, et al. Coordination between ECM and cell-cell adhesion regulates the development of islet aggregation, architecture, and functional maturation. elife. 2023;12: pubmed publisher
Grockowiak E, Korn C, Rak J, Lysenko V, Hallou A, Panvini F, et al. Different niches for stem cells carrying the same oncogenic driver affect pathogenesis and therapy response in myeloproliferative neoplasms. Nat Cancer. 2023;4:1193-1209 pubmed publisher
Wang X, Ramos R, Phan A, Yamaga K, Flesher J, Jiang S, et al. Signalling by senescent melanocytes hyperactivates hair growth. Nature. 2023;618:808-817 pubmed publisher
Baines K, Klausner M, Patterson V, Renaud S. Interleukin-15 deficient rats have reduced osteopontin at the maternal-fetal interface. Front Cell Dev Biol. 2023;11:1079164 pubmed publisher
Raas Q, Tawbeh A, Tahri Joutey M, Gondcaille C, Keime C, Kaiser R, et al. Peroxisomal defects in microglial cells induce a disease-associated microglial signature. Front Mol Neurosci. 2023;16:1170313 pubmed publisher
Boonpraman N, Yoon S, Kim C, Moon J, Yi S. NOX4 as a critical effector mediating neuroinflammatory cytokines, myeloperoxidase and osteopontin, specifically in astrocytes in the hippocampus in Parkinson's disease. Redox Biol. 2023;62:102698 pubmed publisher
Yıldız E, El Alam G, Perino A, Jalil A, Denechaud P, Huber K, et al. Hepatic lipid overload triggers biliary epithelial cell activation via E2Fs. elife. 2023;12: pubmed publisher
Li L, Cui L, Lin P, Liu Z, Bao S, Ma X, et al. Kupffer-cell-derived IL-6 is repurposed for hepatocyte dedifferentiation via activating progenitor genes from injury-specific enhancers. Cell Stem Cell. 2023;30:283-299.e9 pubmed publisher
De Schepper S, Ge J, Crowley G, Ferreira L, Garceau D, Toomey C, et al. Perivascular cells induce microglial phagocytic states and synaptic engulfment via SPP1 in mouse models of Alzheimer's disease. Nat Neurosci. 2023;26:406-415 pubmed publisher
Qiu Y, Shen X, Ravid O, Atrakchi D, Rand D, Wight A, et al. Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease. Proc Natl Acad Sci U S A. 2023;120:e2218915120 pubmed publisher
Bai Q, Wang X, Yan H, Wen L, Zhou Z, Ye Y, et al. Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling. J Pers Med. 2023;13: pubmed publisher
Song Y, Jamali N, Sorenson C, Sheibani N. Vitamin D Receptor Expression Limits the Angiogenic and Inflammatory Properties of Retinal Endothelial Cells. Cells. 2023;12: pubmed publisher
Piekna Przybylska D, Na D, Zhang J, Baker C, Ashton J, White P. Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activation. Front Cell Neurosci. 2022;16:1096872 pubmed publisher
Nagashima D, Ishibashi Y, Kawaguchi S, Furukawa M, Toho M, Ohno M, et al. Human Recombinant Lactoferrin Promotes Differentiation and Calcification on MC3T3-E1 Cells. Pharmaceutics. 2022;15: pubmed publisher
Li S, Jakobs T. Secreted phosphoprotein 1 slows neurodegeneration and rescues visual function in mouse models of aging and glaucoma. Cell Rep. 2022;41:111880 pubmed publisher
Pagani C, Bancroft A, Tower R, Livingston N, Sun Y, Hong J, et al. Discoidin domain receptor 2 regulates aberrant mesenchymal lineage cell fate and matrix organization. Sci Adv. 2022;8:eabq6152 pubmed publisher
Spitzer D, Puetz T, Armbrust M, Dunst M, Macas J, Croll F, et al. Anti-osteopontin therapy leads to improved edema and infarct size in a murine model of ischemic stroke. Sci Rep. 2022;12:20925 pubmed publisher
Fukushima Y, Sakamoto K, Matsuda M, Yoshikai Y, Yagita H, Kitamura D, et al. cis interaction of CD153 with TCR/CD3 is crucial for the pathogenic activation of senescence-associated T cells. Cell Rep. 2022;40:111373 pubmed publisher
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels T, et al. Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro. Nat Commun. 2022;13:5427 pubmed publisher
Hada Y, Uchida H, Umebayashi R, Yoshida M, Wada J. Cilostazol Attenuates AngII-Induced Cardiac Fibrosis in apoE Deficient Mice. Int J Mol Sci. 2022;23: pubmed publisher
Kiryu Seo S, Matsushita R, Tashiro Y, Yoshimura T, Iguchi Y, Katsuno M, et al. Impaired disassembly of the axon initial segment restricts mitochondrial entry into damaged axons. EMBO J. 2022;41:e110486 pubmed publisher
Wang L, You X, Ruan D, Shao R, Dai H, Shen W, et al. TET enzymes regulate skeletal development through increasing chromatin accessibility of RUNX2 target genes. Nat Commun. 2022;13:4709 pubmed publisher
Guo Y, Chomiak A, Hong Y, Lowe C, Kopsidas C, Chan W, et al. Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration. iScience. 2022;25:104519 pubmed publisher
Spitzer D, Gu xe9 rit S, Puetz T, Khel M, Armbrust M, Dunst M, et al. Profiling the neurovascular unit unveils detrimental effects of osteopontin on the blood-brain barrier in acute ischemic stroke. Acta Neuropathol. 2022;144:305-337 pubmed publisher
Jacobs I, Cheng Z, Ralph D, O BRIEN K, Flaman L, Howe J, et al. INZ-701, a recombinant ENPP1 enzyme, prevents ectopic calcification in an Abcc6-/- mouse model of pseudoxanthoma elasticum. Exp Dermatol. 2022;31:1095-1101 pubmed publisher
Staats K, Sosa B, Kuyl E, Niu Y, Suhardi V, Turajane K, et al. Intermittent parathyroid hormone increases stability and improves osseointegration of initially unstable implants. Bone Joint Res. 2022;11:260-269 pubmed publisher
Schlecht A, Wolf J, Boneva S, Prinz G, Braunger B, Wieghofer P, et al. Transcriptional and Distributional Profiling of Microglia in Retinal Angiomatous Proliferation. Int J Mol Sci. 2022;23: pubmed publisher
Lin S, Yin S, Shi J, Yang G, Wen X, Zhang W, et al. Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration. Bioact Mater. 2022;18:116-127 pubmed publisher
Guerra San Juan I, Nash L, Smith K, Leyton Jaimes M, Qian M, Klim J, et al. Loss of mouse Stmn2 function causes motor neuropathy. Neuron. 2022;110:1671-1688.e6 pubmed publisher
Khamissi F, Ning L, Kefaloyianni E, Dun H, Arthanarisami A, Keller A, et al. Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure. Sci Adv. 2022;8:eabm5900 pubmed publisher
Long J, Leinroth A, Liao Y, Ren Y, Mirando A, Nguyen T, et al. Hypertrophic chondrocytes serve as a reservoir for marrow-associated skeletal stem and progenitor cells, osteoblasts, and adipocytes during skeletal development. elife. 2022;11: pubmed publisher
Xu C, Dinh V, Kruse K, Jeong H, Watson E, Adams S, et al. Induction of osteogenesis by bone-targeted Notch activation. elife. 2022;11: pubmed publisher
Lin C, Chen Z, Guo D, Zhou L, Lin S, Li C, et al. Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model. Aging (Albany NY). 2022;14:253-271 pubmed publisher
Aggarwal N, Deerhake M, DiPalma D, Shahi S, Gaggioli M, Mangalam A, et al. Secreted osteopontin from CD4+ T cells limits acute graft-versus-host disease. Cell Rep. 2021;37:110170 pubmed publisher
Nonoyama S, Karakida T, Chiba Ohkuma R, Yamamoto R, Ujiie Y, Nagano T, et al. Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells. Cells. 2021;10: pubmed publisher
Popovics P, Jain A, Skalitzky K, Schroeder E, Ruetten H, Cadena M, et al. Osteopontin Deficiency Ameliorates Prostatic Fibrosis and Inflammation. Int J Mol Sci. 2021;22: pubmed publisher
Zhou S, Dai Q, Huang X, Jin A, Yang Y, Gong X, et al. STAT3 is critical for skeletal development and bone homeostasis by regulating osteogenesis. Nat Commun. 2021;12:6891 pubmed publisher
Gallego Ortega A, Vidal Villegas B, Norte Mu xf1 oz M, Salinas Navarro M, Avil xe9 s Trigueros M, Villegas P xe9 rez M, et al. 7,8-Dihydroxiflavone Maintains Retinal Functionality and Protects Various Types of RGCs in Adult Rats with Optic Nerve Transection. Int J Mol Sci. 2021;22: pubmed publisher
D Souza S, Swygart D, Wienbar S, Upton B, Zhang K, Mackin R, et al. Retinal patterns and the cellular repertoire of neuropsin (Opn5) retinal ganglion cells. J Comp Neurol. 2021;: pubmed publisher
Briones Orta M, Delgado Coello B, Guti xe9 rrez Vidal R, Sosa Garrocho M, Mac xed as Silva M, Mas Oliva J. Quantitative Expression of Key Cancer Markers in the AS-30D Hepatocarcinoma Model. Front Oncol. 2021;11:670292 pubmed publisher
Kingston R, Amin D, Misra S, Gross J, Kuwajima T. Serotonin transporter-mediated molecular axis regulates regional retinal ganglion cell vulnerability and axon regeneration after nerve injury. PLoS Genet. 2021;17:e1009885 pubmed publisher
Streng M, Tetzlaff M, Krook Magnuson E. Distinct Fastigial Output Channels and Their Impact on Temporal Lobe Seizures. J Neurosci. 2021;41:10091-10107 pubmed publisher
Namba M, Kobayashi T, Kohno M, Koyano T, Hirose T, Fukushima M, et al. Creation of X-linked Alport syndrome rat model with Col4a5 deficiency. Sci Rep. 2021;11:20836 pubmed publisher
Kyjacova L, Saup R, Rönsch K, Wallbaum S, Dukowic Schulze S, Foss A, et al. IER2-induced senescence drives melanoma invasion through osteopontin. Oncogene. 2021;40:6494-6512 pubmed publisher
Lee S, Park Y, Paik S, Kim I. Differential Response of Müller Cells and Microglia in a Mouse Retinal Detachment Model and Its Implications in Detached and Non-Detached Regions. Cells. 2021;10: pubmed publisher
Cordero Espinoza L, Dowbaj A, Kohler T, Strauss B, Sarlidou O, Belenguer G, et al. Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation. Cell Stem Cell. 2021;28:1907-1921.e8 pubmed publisher
Sun J, Shin D, Eiseman M, Yallowitz A, Li N, Lalani S, et al. SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts. Nat Commun. 2021;12:4611 pubmed publisher
Lu C, Liu Z, Klement J, Yang D, Merting A, Poschel D, et al. WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape. J Immunother Cancer. 2021;9: pubmed publisher
Jan T, Eltawil Y, Ling A, Chen L, Ellwanger D, Heller S, et al. Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics. Cell Rep. 2021;36:109358 pubmed publisher
Sivaraj K, Jeong H, Dharmalingam B, Zeuschner D, Adams S, Potente M, et al. Regional specialization and fate specification of bone stromal cells in skeletal development. Cell Rep. 2021;36:109352 pubmed publisher
Hara A, Kato K, Ishihara T, Kobayashi H, Asai N, Mii S, et al. Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity. Genes Cells. 2021;26:495-512 pubmed publisher
Berg I, Mohagheghian E, Habing K, Wang N, Underhill G. Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D. Adv Healthc Mater. 2021;10:e2100223 pubmed publisher
Schlecht A, Zhang P, Wolf J, Thien A, Rosmus D, Boneva S, et al. Secreted Phosphoprotein 1 Expression in Retinal Mononuclear Phagocytes Links Murine to Human Choroidal Neovascularization. Front Cell Dev Biol. 2020;8:618598 pubmed publisher
Yuan Q, Gu J, Zhang J, Liu S, Wang Q, Tian T, et al. MyD88 in myofibroblasts enhances colitis-associated tumorigenesis via promoting macrophage M2 polarization. Cell Rep. 2021;34:108724 pubmed publisher
Bailey S, Sroga G, Hoac B, Katsamenis O, Wang Z, Bouropoulos N, et al. The role of extracellular matrix phosphorylation on energy dissipation in bone. elife. 2020;9: pubmed publisher
Yu L, Zheng Y, Liu B, Kang M, Millar J, Rhee D. Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility. PLoS ONE. 2020;15:e0241294 pubmed publisher
Ratzan E, Moon A, Deans M. Fgf8 genetic labeling reveals the early specification of vestibular hair cell type in mouse utricle. Development. 2020;147: pubmed publisher
Li Y, He X, Kawaguchi R, Zhang Y, Wang Q, Monavarfeshani A, et al. Microglia-organized scar-free spinal cord repair in neonatal mice. Nature. 2020;587:613-618 pubmed publisher
Christodoulou Vafeiadou E, Geka C, Ntari L, Kranidioti K, Argyropoulou E, Meier F, et al. Ectopic bone formation and systemic bone loss in a transmembrane TNF-driven model of human spondyloarthritis. Arthritis Res Ther. 2020;22:232 pubmed publisher
Mahmud F, Du Y, Greif E, Boucher T, Dannals R, Mathews W, et al. Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection. J Neuroinflammation. 2020;17:273 pubmed publisher
Remmerie A, Martens L, Thoné T, Castoldi A, Seurinck R, Pavie B, et al. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver. Immunity. 2020;53:641-657.e14 pubmed publisher
Feng H, Xing W, Han Y, Sun J, Kong M, Gao B, et al. Tendon-derived cathepsin K-expressing progenitor cells activate Hedgehog signaling to drive heterotopic ossification. J Clin Invest. 2020;130:6354-6365 pubmed publisher
Gentile S, Kourouklis A, Ryoo H, Underhill G. Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids. Front Bioeng Biotechnol. 2020;8:792 pubmed publisher
Filiberti A, Gmyrek G, Montgomery M, Sallack R, Carr D. Loss of Osteopontin Expression Reduces HSV-1-Induced Corneal Opacity. Invest Ophthalmol Vis Sci. 2020;61:24 pubmed publisher
Fujita H, Kodama T, du Lac S. Modular output circuits of the fastigial nucleus for diverse motor and nonmotor functions of the cerebellar vermis. elife. 2020;9: pubmed publisher
Maldonado M, Serrill J, Shih H. Painting the Pancreas in Three Dimensions: Whole-Mount Immunofluorescence Method. Methods Mol Biol. 2020;2155:193-200 pubmed publisher
Wei Q, Nakahara F, Asada N, Zhang D, Gao X, Xu C, et al. Snai2 Maintains Bone Marrow Niche Cells by Repressing Osteopontin Expression. Dev Cell. 2020;53:503-513.e5 pubmed publisher
Falero Perez J, Sheibani N, Sorenson C. Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells. PLoS ONE. 2020;15:e0232779 pubmed publisher
Lee S, Zhou P, Whyte S, Shin S. Adeno-Associated Virus Serotype 8-Mediated Genetic Labeling of Cholangiocytes in the Neonatal Murine Liver. Pharmaceutics. 2020;12: pubmed publisher
Nardo A, Gr xfc n N, Zeyda M, Dumanic M, Oberhuber G, Rivelles E, et al. Impact of osteopontin on the development of non-alcoholic liver disease and related hepatocellular carcinoma. Liver Int. 2020;40:1620-1633 pubmed publisher
Xu T, Lu Z, Xiao Z, Liu F, Chen Y, Wang Z, et al. Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-ɑvβ6 integrin in liver fibrosis. Cell Death Dis. 2020;11:199 pubmed publisher
Zhou T, Gao B, Fan Y, Liu Y, Feng S, Cong Q, et al. Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-ß-catenin. elife. 2020;9: pubmed publisher
Song J, Pineault K, Dones J, Raines R, Wellik D. Hox genes maintain critical roles in the adult skeleton. Proc Natl Acad Sci U S A. 2020;117:7296-7304 pubmed publisher
Kole C, Brommer B, Nakaya N, Sengupta M, Bonet Ponce L, Zhao T, et al. Activating Transcription Factor 3 (ATF3) Protects Retinal Ganglion Cells and Promotes Functional Preservation After Optic Nerve Crush. Invest Ophthalmol Vis Sci. 2020;61:31 pubmed publisher
Sivaraj K, Dharmalingam B, Mohanakrishnan V, Jeong H, Kato K, Schröder S, et al. YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells. elife. 2020;9: pubmed publisher
Fu D, Wang L, Chouairi F, Rose I, Abetov D, Miller A, et al. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells. Nat Commun. 2020;11:84 pubmed publisher
Ono K, Keller J, López Ramírez O, González Garrido A, Zobeiri O, Chang H, et al. Retinoic acid degradation shapes zonal development of vestibular organs and sensitivity to transient linear accelerations. Nat Commun. 2020;11:63 pubmed publisher
Tran N, Shekhar K, Whitney I, Jacobi A, Benhar I, Hong G, et al. Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes. Neuron. 2019;104:1039-1055.e12 pubmed publisher
Feng D, Ngov C, Henley N, Boufaied N, Gerarduzzi C. Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury. Sci Rep. 2019;9:16736 pubmed publisher
Dong J, Ma Q. In Vivo Activation and Pro-Fibrotic Function of NF-κB in Fibroblastic Cells During Pulmonary Inflammation and Fibrosis Induced by Carbon Nanotubes. Front Pharmacol. 2019;10:1140 pubmed publisher
Katholnig K, Schütz B, Fritsch S, Schörghofer D, Linke M, Sukhbaatar N, et al. Inactivation of mTORC2 in macrophages is a signature of colorectal cancer that promotes tumorigenesis. JCI Insight. 2019;4: pubmed publisher
Jiang D, Burger C, Casasent A, Albrecht N, Li F, Samuel M. Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae. J Comp Neurol. 2019;: pubmed publisher
Zhang H, Liao M, Cao M, Qiu Z, Yan X, Zhou Y, et al. ATRQβ-001 Vaccine Prevents Experimental Abdominal Aortic Aneurysms. J Am Heart Assoc. 2019;8:e012341 pubmed publisher
Honda S, Namekata K, Kimura A, Guo X, Harada C, Murakami A, et al. Survival of Alpha and Intrinsically Photosensitive Retinal Ganglion Cells in NMDA-Induced Neurotoxicity and a Mouse Model of Normal Tension Glaucoma. Invest Ophthalmol Vis Sci. 2019;60:3696-3707 pubmed publisher
van de Vlekkert D, Demmers J, Nguyen X, Campos Y, Machado E, Annunziata I, et al. Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis. Sci Adv. 2019;5:eaav3270 pubmed publisher
Sayyid Z, Wang T, Chen L, Jones S, Cheng A. Atoh1 Directs Regeneration and Functional Recovery of the Mature Mouse Vestibular System. Cell Rep. 2019;28:312-324.e4 pubmed publisher
Severe N, Karabacak N, Gustafsson K, Baryawno N, Courties G, Kfoury Y, et al. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping. Cell Stem Cell. 2019;25:570-583.e7 pubmed publisher
Ji G, Xu R, Niu Y, Li N, Ivashkiv L, Bostrom M, et al. Vascular endothelial growth factor pathway promotes osseointegration and CD31hiEMCNhi endothelium expansion in a mouse tibial implant model: an animal study. Bone Joint J. 2019;101-B:108-114 pubmed publisher
Bray E, Yungher B, Levay K, Ribeiro M, Dvoryanchikov G, Ayupe A, et al. Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion Cells. Neuron. 2019;: pubmed publisher
Quilichini E, Fabre M, Dirami T, Stedman A, De Vas M, Ozguc O, et al. Pancreatic ductal deletion of Hnf1b disrupts exocrine homeostasis, leads to pancreatitis and facilitates tumorigenesis. Cell Mol Gastroenterol Hepatol. 2019;: pubmed publisher
Riew T, Kim S, Jin X, Kim H, Lee J, Lee M. Osteopontin and its spatiotemporal relationship with glial cells in the striatum of rats treated with mitochondrial toxin 3-nitropropionic acid: possible involvement in phagocytosis. J Neuroinflammation. 2019;16:99 pubmed publisher
Pepe Mooney B, Dill M, Alemany A, Ordovas Montanes J, Matsushita Y, Rao A, et al. Single-Cell Analysis of the Liver Epithelium Reveals Dynamic Heterogeneity and an Essential Role for YAP in Homeostasis and Regeneration. Cell Stem Cell. 2019;: pubmed publisher
Romeo S, Alawi K, Rodrigues J, Singh A, Kusumbe A, Ramasamy S. Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation. Nat Cell Biol. 2019;21:430-441 pubmed publisher
Hu H, Liu Z, Liu C. Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients. Oncol Lett. 2019;17:2788-2794 pubmed publisher
Caglayan E, Trappiel M, Behringer A, Berghausen E, Odenthal M, Wellnhofer E, et al. Pulmonary arterial remodelling by deficiency of peroxisome proliferator-activated receptor-γ in murine vascular smooth muscle cells occurs independently of obesity-related pulmonary hypertension. Respir Res. 2019;20:42 pubmed publisher
Wasgewatte Wijesinghe D, Mackie E, Pagel C. Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells. Skelet Muscle. 2019;9:6 pubmed publisher
Giraud E, Rouault E, Fiette L, Colle J, Smirlis D, Melanitou E. Osteopontin in the host response to Leishmania amazonensis. BMC Microbiol. 2019;19:32 pubmed publisher
Zhao M, Tao F, Venkatraman A, Li Z, Smith S, Unruh J, et al. N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells. Cell Rep. 2019;26:652-669.e6 pubmed publisher
Li Q, Cheng Z, Zhou L, Darmanis S, Neff N, Okamoto J, et al. Developmental Heterogeneity of Microglia and Brain Myeloid Cells Revealed by Deep Single-Cell RNA Sequencing. Neuron. 2019;101:207-223.e10 pubmed publisher
Kaylan K, Berg I, Biehl M, Brougham Cook A, Jain I, Jamil S, et al. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling. elife. 2018;7: pubmed publisher
Wang L, Niu N, Li L, Shao R, Ouyang H, Zou W. H3K36 trimethylation mediated by SETD2 regulates the fate of bone marrow mesenchymal stem cells. PLoS Biol. 2018;16:e2006522 pubmed publisher
Lee M, Hwang H, Oh S, Roshanzadeh A, Kim J, Song J, et al. Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression. Exp Mol Med. 2018;50:142 pubmed publisher
Dhande O, Stafford B, Franke K, El Danaf R, Percival K, Phan A, et al. Molecular Fingerprinting of On-Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits. J Neurosci. 2019;39:78-95 pubmed publisher
Falero Perez J, Song Y, Zhao Y, Teixeira L, Sorenson C, Sheibani N. Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells. PLoS ONE. 2018;13:e0206756 pubmed publisher
Jin Y, Cong Q, Gvozdenovic Jeremic J, Hu J, Zhang Y, Terkeltaub R, et al. Enpp1 inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling. Development. 2018;145: pubmed publisher
D Souza A, Jiang Y, Cast A, Valanejad L, Wright M, Lewis K, et al. Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation. Cell Mol Gastroenterol Hepatol. 2018;6:239-255 pubmed publisher
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product information
brand :
R&D Systems
master code :
AF808
SKU :
AF808
product name :
Mouse Osteopontin/OPN Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Mouse Osteopontin/OPN Antibody from R&D Systems is a goat polyclonal antibody to Osteopontin/OPN. This antibody reacts with human,mouse,rat,transgenic mouse,xenograft. The Mouse Osteopontin/OPN Antibody has been validated for the following applications: Immunohistochemistry,Western Blot,Immunocytochemistry,Neutralization,IHC/IF,Bioassay,ELISA Development,ELISA Development (Capture),Flow Cytometry,Immunofluorescence,Cell Culture,ELISA Capture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,RNAscope compatible,Immunocytochemistry/ Immunofluorescence,ELISA Capture (Matched Antibody Pair).
target :
Osteopontin/OPN
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.1 ug/mL, Immunohistochemistry 5-15 ug/mL, Neutralization 1-3 ug/mL, Immunocytochemistry 5-25 ug/mL, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN (R D Systems, Catalog # 441-OP), Leu17-Asn294 (Glu99Gly), Accession # Q547B5
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat,Transgenic Mouse,Xenograft
specificity :
Detects mouse Osteopontin (OPN) in ELISAs and Western blots.
gene symbol :
SPP1
endotoxin note :
<0.30 EU per 1 ╡g of the antibody by the LAL method.
top caption :
Osteopontin/OPN antibody in C2C12 Mouse Cell Line and Mouse Splenocytes by Immunocytochemistry (ICC).
accessionNumbers :
Q547B5
applications :
ELISA Capture (Matched Antibody Pair),Bioassay,ELISA Development,ELISA Development (Capture),IHC/IF,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Neutralization,Immunofluorescence,Immunocytochemistry,RNAscope compatible,Cell Culture,Immunocytochemistry/ Immunofluorescence,ELISA Capture
USD :
499 USD
alt names :
BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, osteopontin, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), Spp1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, uropontin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.