product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse/Rat Tie-2 Antibody
catalog :
AF762
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat, chicken
application :
western blot, immunohistochemistry, neutralization, immunohistochemistry - frozen section, immunohistochemistry - free floating section, immunohistochemistry knockout validation
more info or order :
R&D Systems product webpage
citations: 28
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; loading ...; fig s1b
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
  • immunohistochemistry - frozen section; human; loading ...; fig 2a
Kim J, Park D, Bae H, Park D, Kim D, Lee C, et al. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma. J Clin Invest. 2017;127:3877-3896 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:50; fig 9
  • western blot; mouse; 1:500; fig 8
Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, et al. Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling. Acta Neuropathol. 2016;131:753-73 pubmed publisher
Cash A, de Jager C, Brickler T, Soliman E, Ladner L, Kaloss A, et al. Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood-brain barrier integrity. Proc Natl Acad Sci U S A. 2023;120:e2204700120 pubmed publisher
Koo Y, Lim C, Darai A, Lee J, Kim W, Han I, et al. Shape-memory collagen scaffold combined with hyaluronic acid for repairing intervertebral disc. Biomater Res. 2023;27:26 pubmed publisher
Lee E, Lee E, Kong E, Chon H, Llaiqui Condori M, Park C, et al. An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone. Exp Mol Med. 2023;55:470-484 pubmed publisher
Shirakura K, Baluk P, Nottebaum A, Ipe U, Peters K, McDonald D, et al. Shear stress control of vascular leaks and atheromas through Tie2 activation by VE-PTP sequestration. EMBO Mol Med. 2023;15:e16128 pubmed publisher
Lask A, Gutbier B, Kershaw O, Nouailles G, Gruber A, M xfc ller Redetzky H, et al. Adjunctive therapy with the Tie2 agonist Vasculotide reduces pulmonary permeability in Streptococcus pneumoniae infected and mechanically ventilated mice. Sci Rep. 2022;12:15531 pubmed publisher
Kowalski E, Soliman E, Kelly C, Basso E, Leonard J, Pridham K, et al. Monocyte proinflammatory phenotypic control by ephrin type A receptor 4 mediates neural tissue damage. JCI Insight. 2022;7: pubmed publisher
Bhatia S, Nguyen D, Darragh L, Van Court B, Sharma J, Knitz M, et al. EphB4 and ephrinB2 act in opposition in the head and neck tumor microenvironment. Nat Commun. 2022;13:3535 pubmed publisher
Choe Y, Yoon J, Joo J, Kim B, Hong S, Koh G, et al. Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain. Front Cell Neurosci. 2022;16:848764 pubmed publisher
Zhu J, Li J, Chung C, Lomas Neira J, Ayala A. Patho-Mechanisms for Hemorrhage/Sepsis-Induced Indirect Acute Respiratory Distress Syndrome: A Role for Lung TIE1 and Its Regulation by Neutrophils. Shock. 2022;57:608-615 pubmed publisher
van Leeuwen A, Dekker N, Van Slyke P, De Groot E, Vervloet M, Roelofs J, et al. The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats. Intensive Care Med Exp. 2021;9:23 pubmed publisher
Lee B, Shin H, Oh J, Park J, Park M, Yang S, et al. An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1. Autophagy. 2021;17:1649-1666 pubmed publisher
Park M, Hong S, Park S, Kim Y, Yang S, Kim H, et al. Perivascular Stem Cell-Derived Cyclophilin A Improves Uterine Environment with Asherman's Syndrome via HIF1α-Dependent Angiogenesis. Mol Ther. 2020;28:1818-1832 pubmed publisher
Chatterjee A, Eshwaran R, Huang H, Zhao D, Schmidt M, Wieland T, et al. Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency. Int J Mol Sci. 2020;21: pubmed publisher
Dong W, Li R, Yang H, Lu Y, Zhou L, Sun L, et al. Mesenchymal-endothelial transition-derived cells as a potential new regulatory target for cardiac hypertrophy. Sci Rep. 2020;10:6652 pubmed publisher
Singhal M, Gengenbacher N, La Porta S, Gehrs S, Shi J, Kamiyama M, et al. Preclinical validation of a novel metastasis-inhibiting Tie1 function-blocking antibody. EMBO Mol Med. 2020;12:e11164 pubmed publisher
Li Z, Korhonen E, Merlini A, Strauss J, Wihuri E, Nurmi H, et al. Angiopoietin-2 blockade ameliorates autoimmune neuroinflammation by inhibiting leukocyte recruitment into the CNS. J Clin Invest. 2020;130:1977-1990 pubmed publisher
Chen H, Fu X, Jiang J, Han S. C16 Peptide Promotes Vascular Growth and Reduces Inflammation in a Neuromyelitis Optica Model. Front Pharmacol. 2019;10:1373 pubmed publisher
Qu X, Violette K, Sewell Loftin M, Soslow J, Saint Jean L, Hinton R, et al. Loss of flow responsive Tie1 results in Impaired
Aortic valve remodeling. Dev Biol. 2019;455:73-84 pubmed publisher
Besnier M, Shantikumar S, Anwar M, Dixit P, Chamorro Jorganes A, Sweaad W, et al. miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy System in Limb Ischemia. Mol Ther Nucleic Acids. 2019;17:49-62 pubmed publisher
Pedrosa A, Bodrug N, Gómez Escudero J, Carter E, Reynolds L, Georgiou P, et al. Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861. Cancer Res. 2019;: pubmed publisher
Kim J, Park J, Choi J, Park I, Hwang Y, Bae H, et al. Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration. Sci Adv. 2019;5:eaau6732 pubmed publisher
Kato K, Diéguez Hurtado R, Park D, Hong S, Kato Azuma S, Adams S, et al. Pulmonary pericytes regulate lung morphogenesis. Nat Commun. 2018;9:2448 pubmed publisher
Higgins S, De Ceunynck K, Kellum J, Chen X, Gu X, Chaudhry S, et al. Tie2 protects the vasculature against thrombus formation in systemic inflammation. J Clin Invest. 2018;128:1471-1484 pubmed publisher
Morooka N, Futaki S, Sato Nishiuchi R, Nishino M, Totani Y, Shimono C, et al. Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling. Circ Res. 2017;120:1276-1288 pubmed publisher
Zhou Y, Williams J, Smallwood P, Nathans J. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina. PLoS ONE. 2015;10:e0143650 pubmed publisher
Hakanpaa L, Sipilä T, Leppänen V, Gautam P, Nurmi H, Jacquemet G, et al. Endothelial destabilization by angiopoietin-2 via integrin β1 activation. Nat Commun. 2015;6:5962 pubmed publisher
product information
master code :
AF762
SKU :
AF762
product name :
Mouse/Rat Tie-2 Antibody
unit size :
100 ug (also 25 ug)
description :
The Mouse/Rat Tie-2 Antibody from R&D Systems is a goat polyclonal antibody to Tie-2. This antibody reacts with avian - chicken,human,mouse,rat,transgenic mouse. The Mouse/Rat Tie-2 Antibody has been validated for the following applications: Immunocytochemistry/Immunofluorescence,Immunohistochemistry-Frozen,Western Blot,Neutralization,Immunohistochemistry.
target :
Tie-2
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse Tie-2, Ala23-Lys744, Accession # CAA47857
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Avian - Chicken,Human,Mouse,Rat,Transgenic Mouse
specificity :
Detects mouse and rat Tie-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) Tie-2 is observed and less than 1% cross-reactivity with recombinant zebrafish Tie-2 and rhTie-1 is observed.
gene symbol :
TEK
top caption :
Tie-2 antibody in Human Intestine Tissue by Immunohistochemistry (IHC-P).
accessionNumbers :
CAA47857
applications :
Neutralization,Western Blot,Immunohistochemistry,Immunohistochemistry-Frozen
USD :
509 USD
alt names :
angiopoietin-1 receptor, CD202B, CD202b antigen, EC 2.7.10, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, TEK, TEK tyrosine kinase, endothelial, Tie2, TIE-2, TIE2CD202b, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.