product summary
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company name :
R&D Systems
product type :
antibody
product name :
Human/Mouse E-Cadherin Antibody
catalog :
AF648
quantity :
100 ug (also 25 ug)
price :
549 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, dogs, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 58
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:600; fig 2a
Sarvestani S, SIGNS S, Hu B, Yeu Y, Feng H, Ni Y, et al. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity. Nat Commun. 2021;12:262 pubmed publisher
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Tan Y, Flynn W, Sivajothi S, Luo D, Bozal S, Dav xe9 M, et al. Single-cell analysis of endometriosis reveals a coordinated transcriptional programme driving immunotolerance and angiogenesis across eutopic and ectopic tissues. Nat Cell Biol. 2022;24:1306-1318 pubmed publisher
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Hamade H, Stamps J, Stamps D, More S, Thomas L, Blackwood A, et al. BATF3 Protects Against Metabolic Syndrome and Maintains Intestinal Epithelial Homeostasis. Front Immunol. 2022;13:841065 pubmed publisher
Olmsted Z, Paluh J. A combined human gastruloid model of cardiogenesis and neurogenesis. iScience. 2022;25:104486 pubmed publisher
Koide T, Koyanagi Aoi M, Uehara K, Kakeji Y, Aoi T. CDX2-induced intestinal metaplasia in human gastric organoids derived from induced pluripotent stem cells. iScience. 2022;25:104314 pubmed publisher
Hong Y, Shan S, Gu Y, Huang H, Zhang Q, Han Y, et al. Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica. Nat Commun. 2022;13:1309 pubmed publisher
Kiss E, Forika G, Dank M, Krenacs T, Nemeth Z. Methyl Donors Reduce Cell Proliferation by Diminishing Erk-Signaling and NFkB Levels, While Increasing E-Cadherin Expression in Panc-1 Cell Line. Int J Mol Sci. 2022;23: pubmed publisher
Uehara K, Koyanagi Aoi M, Koide T, Itoh T, Aoi T. Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids. Stem Cell Reports. 2022;17:820-834 pubmed publisher
Estrada H, Patel S, RABIZADEH S, Casero D, Targan S, Barrett R. Development of a Personalized Intestinal Fibrosis Model Using Human Intestinal Organoids Derived From Induced Pluripotent Stem Cells. Inflamm Bowel Dis. 2022;28:667-679 pubmed publisher
Eicher A, Kechele D, Sundaram N, Berns H, Poling H, Haines L, et al. Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells. Cell Stem Cell. 2022;29:36-51.e6 pubmed publisher
Yucer N, Ahdoot R, Workman M, Laperle A, Recouvreux M, Kurowski K, et al. Human iPSC-derived fallopian tube organoids with BRCA1 mutation recapitulate early-stage carcinogenesis. Cell Rep. 2021;37:110146 pubmed publisher
Ruiz Torres S, Lambert P, Wikenheiser Brokamp K, Wells S. Directed differentiation of human pluripotent stem cells into epidermal stem and progenitor cells. Mol Biol Rep. 2021;48:6213-6222 pubmed publisher
Bedard M, de Alarcon A, Kou Y, Lee D, Sestito A, Duggins A, et al. HPV Strain Predicts Severity of Juvenile-Onset Recurrent Respiratory Papillomatosis with Implications for Disease Screening. Cancers (Basel). 2021;13: pubmed publisher
Zhang Y, Devocelle A, Desterke C, de Souza L, Hadadi x, Acloque H, et al. BMAL1 Knockdown Leans Epithelial-Mesenchymal Balance toward Epithelial Properties and Decreases the Chemoresistance of Colon Carcinoma Cells. Int J Mol Sci. 2021;22: pubmed publisher
Zolghadr F, Tse N, Loka D, Joun G, Meppat S, Wan V, et al. A Wnt-mediated phenotype switch along the epithelial-mesenchymal axis defines resistance and invasion downstream of ionising radiation in oral squamous cell carcinoma. Br J Cancer. 2021;124:1921-1933 pubmed publisher
Koike H, Iwasawa K, Ouchi R, Maezawa M, Kimura M, Kodaka A, et al. Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells. Nat Protoc. 2021;16:919-936 pubmed publisher
Bedard M, Brusadelli M, Carlile A, Ruiz Torres S, Lodin H, Lee D, et al. Patient-Derived Organotypic Epithelial Rafts Model Phenotypes in Juvenile-Onset Recurrent Respiratory Papillomatosis. Viruses. 2021;13: pubmed publisher
Workman M, Troisi E, Targan S, Svendsen C, Barrett R. Modeling Intestinal Epithelial Response to Interferon-γ in Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids. Int J Mol Sci. 2020;22: pubmed publisher
Janssen A, Duivenvoorde L, Rijkers D, Nijssen R, Peijnenburg A, Van Der Zande M, et al. Cytochrome P450 expression, induction and activity in human induced pluripotent stem cell-derived intestinal organoids and comparison with primary human intestinal epithelial cells and Caco-2 cells. Arch Toxicol. 2021;95:907-922 pubmed publisher
Chen L, Dalton S. Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells. STAR Protoc. 2020;1:100031 pubmed publisher
Soleimany A, Kirkpatrick J, Su S, Dudani J, Zhong Q, Bekdemir A, et al. Activatable Zymography Probes Enable In Situ Localization of Protease Dysregulation in Cancer. Cancer Res. 2021;81:213-224 pubmed publisher
Caron T, Scott K, Sinha N, Muthupalani S, Baqai M, Ang L, et al. Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells. Cell Mol Gastroenterol Hepatol. 2021;11:783-801 pubmed publisher
Liu J, Pan T, Chen Y, Liu Y, Yang F, Chen Q, et al. Repair of acute liver damage with immune evasive hESC derived hepato-blasts. Stem Cell Res. 2020;49:102010 pubmed publisher
Alexander K, Serrano C, Chakraborty A, Nearing M, Council L, Riquelme A, et al. Modulation of glycosyltransferase ST6Gal-I in gastric cancer-derived organoids disrupts homeostatic epithelial cell turnover. J Biol Chem. 2020;295:14153-14163 pubmed publisher
Dabelsteen S, Pallesen E, Marinova I, Nielsen M, Adamopoulou M, Rømer T, et al. Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model. Dev Cell. 2020;54:669-684.e7 pubmed publisher
Dias A, Lozovska A, Wymeersch F, Novoa A, Binagui Casas A, Sobral D, et al. A Tgfbr1/Snai1-dependent developmental module at the core of vertebrate axial elongation. elife. 2020;9: pubmed publisher
Thompson W, Takebe T. Generation of multi-cellular human liver organoids from pluripotent stem cells. Methods Cell Biol. 2020;159:47-68 pubmed publisher
Daoud A, Múnera J. Generation of human colonic organoids from human pluripotent stem cells. Methods Cell Biol. 2020;159:201-227 pubmed publisher
Koterazawa Y, Koyanagi Aoi M, Uehara K, Kakeji Y, Aoi T. Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium. J Gastroenterol. 2020;55:763-774 pubmed publisher
Lakshmi Narayan P, Kajon A. Human mastadenovirus-B (HAdV-B)-specific E3-CR1β and E3-CR1γ glycoproteins interact with each other and localize at the plasma membrane of non-polarized airway epithelial cells. Virology. 2020;546:67-78 pubmed publisher
Rao W, Wang S, Duleba M, Niroula S, Goller K, Xie J, et al. Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis. Cell. 2020;181:848-864.e18 pubmed publisher
Cui W, Sathyanarayan A, Lopresti M, Aghajan M, Chen C, Mashek D. Lipophagy-derived fatty acids undergo extracellular efflux via lysosomal exocytosis. Autophagy. 2020;:1-16 pubmed publisher
Kline K, Lian H, Zhong X, Luo X, Winston J, Cong Y, et al. Neonatal Injury Increases Gut Permeability by Epigenetically Suppressing E-Cadherin in Adulthood. J Immunol. 2020;204:980-989 pubmed publisher
Praveschotinunt P, Duraj Thatte A, Gelfat I, Bahl F, Chou D, Joshi N. Engineered E. coli Nissle 1917 for the delivery of matrix-tethered therapeutic domains to the gut. Nat Commun. 2019;10:5580 pubmed publisher
Graf K, Last A, Gratz R, Allert S, Linde S, Westermann M, et al. Keeping Candida commensal: how lactobacilli antagonize pathogenicity of Candida albicans in an in vitro gut model. Dis Model Mech. 2019;12: pubmed publisher
Devocelle A, Lecru L, Francois H, Desterke C, Gallerne C, Eid P, et al. Inhibition of TGF-β1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-β1-Induced EMT in Renal Fibrosis. Int J Cell Biol. 2019;2019:9151394 pubmed publisher
Kiener M, Chen L, Krebs M, Grosjean J, Klima I, Kalogirou C, et al. miR-221-5p regulates proliferation and migration in human prostate cancer cells and reduces tumor growth in vivo. BMC Cancer. 2019;19:627 pubmed publisher
Colunga T, Hayworth M, Kreß S, Reynolds D, Chen L, Nazor K, et al. Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair. Cell Rep. 2019;26:2566-2579.e10 pubmed publisher
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Yucer N, Holzapfel M, Jenkins Vogel T, Lenaeus L, Ornelas L, Laury A, et al. Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium. Sci Rep. 2017;7:10741 pubmed publisher
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product information
brand :
R&D Systems
master code :
AF648
SKU :
AF648
product name :
Human/Mouse E-Cadherin Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Human/Mouse E-Cadherin Antibody from R&D Systems is a goat polyclonal antibody to E-Cadherin. This antibody reacts with canine,human,mouse,ovine,primate - macaca mulatta (rhesus macaque),transgenic mouse. The Human/Mouse E-Cadherin Antibody has been validated for the following applications: Western Blot,Immunocytochemistry,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Flow Cytometry,Neutralization,Immunohistochemistry-Paraffin,Simple Western,CyTOF-ready,Dual RNAscope ISH-IHC Compatible.
target :
E-Cadherin
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.5 ug/mL, Simple Western 5 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 0.3-15 ug/mL, Immunocytochemistry 5-15 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 0.3-15 ug/mL
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant human E-Cadherin, Asp155-Ile707, Accession # P12830
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Canine,Human,Mouse,Ovine,Primate - Macaca mulatta (Rhesus Macaque),Transgenic Mouse
specificity :
Detects human E-Cadherin in direct ELISAs and Western blots.
gene symbol :
CDH1
Antibody validation :
Orthogonal Validation
top caption :
Detection of Human and Mouse E-Cadherin antibody by Western Blot.
accessionNumbers :
P12830
applications :
Simple Western,CyTOF-ready,Dual RNAscope ISH-IHC Compatible,Flow Cytometry,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry
USD :
549 USD
alt names :
Arc-1, CAD1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CD324 antigen, CDH1, CDHE, cell-CAM 120/80, Cell-CAM120/80, ECAD, E-cadherin, ECadherin, Epithelial cadherin, LCAM, L-CAM, UVOE-Cadherin, uvomorulin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.