product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse/Rat Wnt-5a Antibody
catalog :
AF645
quantity :
100 ug (also 25 ug)
price :
489 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
R&D Systems product webpage
citations: 27
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 7a
Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J. 2018;37: pubmed publisher
  • western blot; human; 1:300; loading ...; fig s3d
Dass R, Sarshad A, Carson B, Feenstra J, Kaur A, Obrdlik A, et al. Wnt5a Signals through DVL1 to Repress Ribosomal DNA Transcription by RNA Polymerase I. PLoS Genet. 2016;12:e1006217 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:20; loading ...; fig s2e
Cantú A, Altshuler Keylin S, Laird D. Discrete somatic niches coordinate proliferation and migration of primordial germ cells via Wnt signaling. J Cell Biol. 2016;214:215-29 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 3
Skaria T, Burgener J, Bächli E, Schoedon G. IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling. PLoS ONE. 2016;11:e0156002 pubmed publisher
Ahmad S, Attisano L. Wnt5a Promotes Axon Elongation in Coordination with the Wnt-Planar Cell Polarity Pathway. Cells. 2024;13: pubmed publisher
Mehdawi L, Ghatak S, Chakraborty P, Sj xf6 lander A, Andersson T. LGR5 Expression Predicting Poor Prognosis Is Negatively Correlated with WNT5A in Colon Cancer. Cells. 2023;12: pubmed publisher
Patel J, Joel M, Lee K, Kambala A, Cornman H, Oladipo O, et al. Single-cell RNA sequencing reveals dysregulated fibroblast subclusters in prurigo nodularis. bioRxiv. 2023;: pubmed publisher
Thomson C, Pundavela J, Perrino M, Coover R, Choi K, Chaney K, et al. WNT5A inhibition alters the malignant peripheral nerve sheath tumor microenvironment and enhances tumor growth. Oncogene. 2021;40:4229-4241 pubmed publisher
van Vliet A, Lee J, van der Poel M, Mason M, Noordermeer J, Fradkin L, et al. Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injury. PLoS ONE. 2021;16:e0249748 pubmed publisher
Lund C, Dyhl Polk A, Nielsen D, Riis L. Wnt5a expression and prognosis in stage II-III colon cancer. Transl Oncol. 2021;14:100892 pubmed publisher
Inestrosa N, Tapia Rojas C, Lindsay C, Zolezzi J. Wnt Signaling Pathway Dysregulation in the Aging Brain: Lessons From the Octodon degus. Front Cell Dev Biol. 2020;8:734 pubmed publisher
Kawarazaki W, Mizuno R, Nishimoto M, Ayuzawa N, Hirohama D, Ueda K, et al. Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency. J Clin Invest. 2020;130:4152-4166 pubmed publisher
Ikezu S, Yeh H, Delpech J, Woodbury M, Van Enoo A, Ruan Z, et al. Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities. Mol Psychiatry. 2020;: pubmed publisher
Wu X, Van Dijk E, Ng Blichfeldt J, Bos I, Ciminieri C, Konigshoff M, et al. Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors. Cells. 2019;8: pubmed publisher
Mehmeti M, Bergenfelz C, Källberg E, Millrud C, Björk P, Ivars F, et al. Wnt5a is a TLR2/4-ligand that induces tolerance in human myeloid cells. Commun Biol. 2019;2:176 pubmed publisher
Gignac S, Hosseini Farahabadi S, Akazawa T, Schuck N, Fu K, Richman J. Robinow syndrome skeletal phenotypes caused by the WNT5AC83S variant are due to dominant interference with chondrogenesis. Hum Mol Genet. 2019;28:2395-2414 pubmed publisher
Oliveira L, Oshima C, Soffner P, Silva M, Lins R, Malinverni A, et al. THE CANONICAL WNT PATHWAY IN GASTRIC CARCINOMA. Arq Bras Cir Dig. 2019;32:e1414 pubmed publisher
Han C, Li J, Wang C, Ouyang H, Ding X, Liu Y, et al. Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling Pathway. Invest Ophthalmol Vis Sci. 2018;59:3449-3460 pubmed publisher
Meo Burt P, Xiao L, Hurley M. FGF23 Regulates Wnt/β-Catenin Signaling-Mediated Osteoarthritis in Mice Overexpressing High-Molecular-Weight FGF2. Endocrinology. 2018;159:2386-2396 pubmed publisher
Xu J, Li X, Cole A, Sherman Z, Du W. Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis-Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells. Stem Cells. 2018;36:785-795 pubmed publisher
Susman M, Karuna E, Kunz R, Gujral T, Cantú A, Choi S, et al. Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates. elife. 2017;6: pubmed publisher
Chen C, Orefice L, Chiu S, Legates T, Hattar S, Huganir R, et al. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice. Proc Natl Acad Sci U S A. 2017;114:E619-E628 pubmed publisher
Baarsma H, Skronska Wasek W, Mutze K, Ciolek F, Wagner D, John Schuster G, et al. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD. J Exp Med. 2017;214:143-163 pubmed publisher
Uchiyama K, Sakiyama T, Hasebe T, Musch M, Miyoshi H, Nakagawa Y, et al. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development. Sci Rep. 2016;6:32094 pubmed publisher
Ramirez V, Ramos Fernández E, Henriquez J, Lorenzo A, Inestrosa N. Wnt-5a/Frizzled9 Receptor Signaling through the G?o-G?? Complex Regulates Dendritic Spine Formation. J Biol Chem. 2016;291:19092-107 pubmed publisher
Skaria T, Bächli E, Schoedon G. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells. Cell Adh Migr. 2017;11:24-38 pubmed publisher
Rider V, Talbott A, Bhusri A, Krumsick Z, Foster S, Wormington J, et al. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation. J Endocrinol. 2016;229:197-207 pubmed publisher
product information
master code :
AF645
SKU :
AF645
product name :
Mouse/Rat Wnt-5a Antibody
unit size :
100 ug (also 25 ug)
description :
The Mouse/Rat Wnt-5a Antibody from R&D Systems is a goat polyclonal antibody to Wnt-5a. This antibody reacts with human,mouse,rat,frog - xenopus (african clawed frog). The Mouse/Rat Wnt-5a Antibody has been validated for the following applications: Immunocytochemistry,Western Blot,Bioassay,Blocking,Immunodepletion,Neutralization,ELISA Detection,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen.
target :
Wnt-5a
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
E. coli -derived recombinant mouse Wnt-5a peptide, Gln254-Cys334, Accession # P22725
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Human,Mouse,Rat,Frog - Xenopus (African Clawed Frog)
specificity :
Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt-5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.
gene symbol :
WNT5A
top caption :
Wnt-5a antibody in Mouse Embryonic Rib by Immunohistochemistry (IHC-P).
accessionNumbers :
P22725
applications :
Western Blot,Immunocytochemistry,Bioassay,Blocking,Immunodepletion,Neutralization,ELISA Detection,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen
USD :
489 USD
alt names :
hWNT5A, protein Wnt-5a, wingless-type MMTV integration site family, member 5A, Wnt5a, WNT-5A protein
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.