product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse VEGFR2/KDR/Flk-1 Antibody
catalog :
AF644
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, proximity ligation assay, immunohistochemistry knockout validation
more info or order :
citations: 55
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; loading ...; fig s3c
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3c
Kim J, Park D, Bae H, Park D, Kim D, Lee C, et al. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma. J Clin Invest. 2017;127:3877-3896 pubmed publisher
  • proximity ligation assay; mouse; 1:50; loading ...; fig 3a
Harde E, Nicholson L, Furones Cuadrado B, Bissen D, Wigge S, Urban S, et al. EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development. elife. 2019;8: pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 7j
Diéguez Hurtado R, Kato K, Giaimo B, Nieminen Kelhä M, Arf H, Ferrante F, et al. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019;10:2817 pubmed publisher
  • proximity ligation assay; human; 1:80; fig 2b
Segarra M, Aburto M, Cop F, Llaó Cid C, Härtl R, Damm M, et al. Endothelial Dab1 signaling orchestrates neuro-glia-vessel communication in the central nervous system. Science. 2018;361: pubmed publisher
  • immunohistochemistry - free floating section; mouse; fig s4a
Thion M, Low D, Silvin A, Chen J, Grisel P, Schulte Schrepping J, et al. Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner. Cell. 2018;172:500-516.e16 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig s2d
Hirai M, Arita Y, McGlade C, Lee K, Chen J, Evans S. Adaptor proteins NUMB and NUMBL promote cell cycle withdrawal by targeting ERBB2 for degradation. J Clin Invest. 2017;127:569-582 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1b
Wang X, Chen D, Chen K, Jubran A, Ramirez A, Astrof S. Endothelium in the pharyngeal arches 3, 4 and 6 is derived from the second heart field. Dev Biol. 2017;421:108-117 pubmed publisher
  • immunohistochemistry; mouse; 1:300; loading ...; fig 5c
Lucitti J, Sealock R, Buckley B, Zhang H, Xiao L, Dudley A, et al. Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke. Stroke. 2016;47:3022-3031 pubmed
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3p
Huang J, Woolf A, Kolatsi Joannou M, Baluk P, Sandford R, Peters D, et al. Vascular Endothelial Growth Factor C for Polycystic Kidney Diseases. J Am Soc Nephrol. 2016;27:69-77 pubmed publisher
Wang H, Barry K, Zaini A, Coakley G, Moyat M, Daunt C, et al. Helminth infection driven gastrointestinal hypermotility is independent of eosinophils and mediated by alterations in smooth muscle instead of enteric neurons. PLoS Pathog. 2024;20:e1011766 pubmed publisher
Jannaway M, Iyer D, Mastrogiacomo D, Li K, Sung D, Yang Y, et al. VEGFR3 is required for button junction formation in lymphatic vessels. Cell Rep. 2023;42:112777 pubmed publisher
Arolkar G, Kumar S, Wang H, Gonzalez K, Kumar S, Bishnoi B, et al. Dedifferentiation and Proliferation of Artery Endothelial Cells Drive Coronary Collateral Development in Mice. Arterioscler Thromb Vasc Biol. 2023;43:1455-1477 pubmed publisher
Wang J, Rattner A, Nathans J. Bacterial meningitis in the early postnatal mouse studied at single-cell resolution. elife. 2023;12: pubmed publisher
Mueller Diesing F, Lederle W, Rix A, Koletnik S, Doleschel D, Snelting M, et al. Molecular Ultrasound Imaging Depicts the Modulation of Tumor Angiogenesis by Acetylsalicylic Acid. Int J Mol Sci. 2023;24: pubmed publisher
Wang C, Wu Y, Li Y, Zhang Y, Fung Y, Tong K, et al. Smad4-mediated angiogenesis facilitates the beiging of white adipose tissue in mice. iScience. 2023;26:106272 pubmed publisher
Lee E, Lee E, Kong E, Chon H, Llaiqui Condori M, Park C, et al. An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone. Exp Mol Med. 2023;55:470-484 pubmed publisher
Liu C, Yemanyi F, Bora K, Kushwah N, Blomfield A, Kamenecka T, et al. Genetic deficiency and pharmacological modulation of RORα regulate laser-induced choroidal neovascularization. Aging (Albany NY). 2023;15:37-52 pubmed publisher
Hsu J, Chong C, Serrill J, Goon L, Balayan J, Johnson E, et al. Preclinical Characterization of XL092, a Novel Receptor Tyrosine Kinase Inhibitor of MET, VEGFR2, AXL, and MER. Mol Cancer Ther. 2023;22:179-191 pubmed publisher
Heydarian M, Oak P, Zhang X, Kamgari N, Kindt A, Koschlig M, et al. Relationship between impaired BMP signalling and clinical risk factors at early-stage vascular injury in the preterm infant. Thorax. 2022;77:1176-1186 pubmed publisher
Baluk P, McDonald D. Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts. Methods Mol Biol. 2022;2441:115-134 pubmed publisher
Karaman S, Paavonsalo S, Heinolainen K, Lackman M, Ranta A, Hemanthakumar K, et al. Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance. J Exp Med. 2022;219: pubmed publisher
Yang C, Feng X, Li Z, He Q. Plumbagin inhibits tumor angiogenesis of gastric carcinoma in mice by modulating nuclear factor-kappa B pathway. Transl Cancer Res. 2020;9:556-564 pubmed publisher
Arpino J, Yin H, Prescott E, Staples S, Nong Z, Li F, et al. Low-flow intussusception and metastable VEGFR2 signaling launch angiogenesis in ischemic muscle. Sci Adv. 2021;7:eabg9509 pubmed publisher
Progatzky F, Shapiro M, Chng S, García Cassani B, Classon C, Sevgi S, et al. Regulation of intestinal immunity and tissue repair by enteric glia. Nature. 2021;599:125-130 pubmed publisher
Lanitis E, Kosti P, Ronet C, Cribioli E, Rota G, Spill A, et al. VEGFR-2 redirected CAR-T cells are functionally impaired by soluble VEGF-A competition for receptor binding. J Immunother Cancer. 2021;9: pubmed publisher
Morgani S, Su J, Nichols J, Massague J, Hadjantonakis A. The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos. elife. 2021;10: pubmed publisher
Yeom D, Lee Y, Ryu I, Lee S, Sung B, Lee H, et al. ABL001, a Bispecific Antibody Targeting VEGF and DLL4, with Chemotherapy, Synergistically Inhibits Tumor Progression in Xenograft Models. Int J Mol Sci. 2020;22: pubmed publisher
Nitzsche A, Pietilä R, Love D, Testini C, Ninchoji T, Smith R, et al. Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis. EMBO Rep. 2021;22:e50218 pubmed publisher
Gula G, Ruminski S, Niderla Bielinska J, Jasińska A, Kiernozek E, Jankowska Steifer E, et al. Potential functions of embryonic cardiac macrophages in angiogenesis, lymphangiogenesis and extracellular matrix remodeling. Histochem Cell Biol. 2021;155:117-132 pubmed publisher
Hong S, Yang M, Cho H, Park I, Bae H, Choe K, et al. Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi. Nat Commun. 2020;11:4102 pubmed publisher
Su L, Lei X, Ma H, Feng C, Jiang J, Jiao J. PRDM16 orchestrates angiogenesis via neural differentiation in the developing brain. Cell Death Differ. 2020;27:2313-2329 pubmed publisher
Luck R, Urban S, Karakatsani A, Harde E, Sambandan S, Nicholson L, et al. VEGF/VEGFR2 signaling regulates hippocampal axon branching during development. elife. 2019;8: pubmed publisher
Duc D, Vigne S, Bernier Latmani J, Yersin Y, Ruiz F, Gaïa N, et al. Disrupting Myelin-Specific Th17 Cell Gut Homing Confers Protection in an Adoptive Transfer Experimental Autoimmune Encephalomyelitis. Cell Rep. 2019;29:378-390.e4 pubmed publisher
Morales Navarrete H, Nonaka H, Scholich A, Segovia Miranda F, de Back W, Meyer K, et al. Liquid-crystal organization of liver tissue. elife. 2019;8: pubmed publisher
Hendrikx S, Coso S, Prat Luri B, Wetterwald L, Sabine A, Franco C, et al. Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2. Cell Rep. 2019;26:1227-1241.e6 pubmed publisher
Das S, Goldstone A, Wang H, Farry J, D Amato G, Paulsen M, et al. A Unique Collateral Artery Development Program Promotes Neonatal Heart Regeneration. Cell. 2019;176:1128-1142.e18 pubmed publisher
FARBER G, Parks M, Lustgarten Guahmich N, Zhang Y, Monette S, Blanchard S, et al. ADAM10 controls the differentiation of the coronary arterial endothelium. Angiogenesis. 2019;22:237-250 pubmed publisher
Choquet C, Nguyen T, Sicard P, Buttigieg E, Tran T, Kober F, et al. Deletion of Nkx2-5 in trabecular myocardium reveals the developmental origins of pathological heterogeneity associated with ventricular non-compaction cardiomyopathy. PLoS Genet. 2018;14:e1007502 pubmed publisher
Su T, Stanley G, Sinha R, D Amato G, Das S, Rhee S, et al. Single-cell analysis of early progenitor cells that build coronary arteries. Nature. 2018;559:356-362 pubmed publisher
Rosin J, Kurrasch D. In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus. J Neuroinflammation. 2018;15:181 pubmed publisher
Kim I, Kim K, Lee E, Oh D, Park C, Park S, et al. Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality. J Exp Med. 2018;215:963-983 pubmed publisher
Rhee S, Chung J, King D, D Amato G, Paik D, Duan A, et al. Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease. Nat Commun. 2018;9:368 pubmed publisher
Drummond C, Hanna J, Garcia M, Devine D, Heyrana A, Finkelstein D, et al. Hedgehog Pathway Drives Fusion-Negative Rhabdomyosarcoma Initiated From Non-myogenic Endothelial Progenitors. Cancer Cell. 2018;33:108-124.e5 pubmed publisher
Sones J, Merriam A, Seffens A, Brown Grant D, Butler S, Zhao A, et al. Angiogenic factor imbalance precedes complement deposition in placentae of the BPH/5 model of preeclampsia. FASEB J. 2017;: pubmed publisher
Chen Y, Xu H, Lin G. Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse. Cell Discov. 2017;3:17046 pubmed publisher
Chang A, Raftrey B, D Amato G, Surya V, Poduri A, Chen H, et al. DACH1 stimulates shear stress-guided endothelial cell migration and coronary artery growth through the CXCL12-CXCR4 signaling axis. Genes Dev. 2017;31:1308-1324 pubmed publisher
Wu H, Zhao J, Fu B, Yin S, Song C, Zhang J, et al. Retinoic acid-induced upregulation of miR-219 promotes the differentiation of embryonic stem cells into neural cells. Cell Death Dis. 2017;8:e2953 pubmed publisher
Heinolainen K, Karaman S, D Amico G, Tammela T, Sormunen R, Eklund L, et al. VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling. Circ Res. 2017;120:1414-1425 pubmed publisher
Langen U, Pitulescu M, Kim J, Enriquez Gasca R, Sivaraj K, Kusumbe A, et al. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. Nat Cell Biol. 2017;19:189-201 pubmed publisher
Ulvmar M, Martínez Corral I, Stanczuk L, Makinen T. Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins. Genesis. 2016;54:350-8 pubmed publisher
Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, et al. Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. J Clin Invest. 2015;125:2661-76 pubmed publisher
Rama N, Dubrac A, Mathivet T, Ní Chárthaigh R, Genet G, Cristofaro B, et al. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med. 2015;21:483-91 pubmed publisher
Hou S, Nilchi L, Li X, Gangaraju S, Jiang S, Aylsworth A, et al. Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage. Sci Rep. 2015;5:7890 pubmed publisher
Wirzenius M, Tammela T, Uutela M, He Y, Odorisio T, Zambruno G, et al. Distinct vascular endothelial growth factor signals for lymphatic vessel enlargement and sprouting. J Exp Med. 2007;204:1431-40 pubmed
Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A, et al. VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. J Cell Biol. 2003;161:1163-77 pubmed
product information
master code :
AF644
SKU :
AF644
product name :
Mouse VEGFR2/KDR/Flk-1 Antibody
unit size :
100 ug (also 25 ug)
description :
The Mouse VEGFR2/KDR/Flk-1 Antibody from R&D Systems is a goat polyclonal antibody to VEGFR2/KDR/Flk-1. This antibody reacts with canine,human,mouse,rat,transgenic mouse. The Mouse VEGFR2/KDR/Flk-1 Antibody has been validated for the following applications: Immunohistochemistry,Immunoprecipitation,Immunocytochemistry,Neutralization,Western Blot,Bioassay,ELISA Development,Flow Cytometry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,CyTOF-ready,Dual RNAscope ISH-IHC Compatible.
target :
VEGFR2/KDR/Flk-1
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant mouse VEGFR2, Ala20-Glu762, Accession # P35918
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Canine,Human,Mouse,Rat,Transgenic Mouse
specificity :
Detects mouse VEGFR2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VEGFR2 is observed and less than 2% cross-reactivity with recombinant mouse (rm) VEGFR1 and rmVEGFR3 is observed.
gene symbol :
KDR
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
top caption :
VEGFR2/KDR/Flk-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).
accessionNumbers :
P35918
applications :
Immunoprecipitation,Neutralization,Immunocytochemistry,Immunohistochemistry,Western Blot,Bioassay,ELISA Development,Flow Cytometry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,CyTOF-ready,Dual RNAscope ISH-IHC Compatible
USD :
509 USD
alt names :
CD309, CD309 antigen, EC 2.7.10, EC 2.7.10.1, Fetal liver kinase 1, fetal liver kinase-1, Flk1, Flk-1, FLK1tyrosine kinase growth factor receptor, KDR, Kinase insert domain receptor, kinase insert domain receptor (a type III receptor tyrosine kinase), KRD1, Ly73, Protein-tyrosine kinase receptor flk-1, soluble VEGFR2, vascular endothelial growth factor receptor 2, VEGF R2, VEGFR, VEGFR2, VEGFR-2
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.