product summary
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company name :
R&D Systems
product type :
antibody
product name :
Mouse/Rat Neuropilin-1 Antibody
catalog :
AF566
quantity :
100 ug (also 25 ug)
price :
509 USD
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat, chicken
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 41
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; mouse; loading ...; fig 1c, 1e
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2a, 2d
Sherafat A, Pfeiffer F, Reiss A, Wood W, Nishiyama A. Microglial neuropilin-1 promotes oligodendrocyte expansion during development and remyelination by trans-activating platelet-derived growth factor receptor. Nat Commun. 2021;12:2265 pubmed publisher
Barnkob M, Michaels Y, Andr xe9 V, Macklin P, Gileadi U, Valvo S, et al. Semmaphorin 3 A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells. Nat Commun. 2024;15:3173 pubmed publisher
Hani T, Fujita K, Kudo T, Taya Y, Sato K, Soeno Y. Tissue-Targeted Transcriptomics Reveals SEMA3D Control of Hypoglossal Nerve Projection to Mouse Tongue Primordia. Acta Histochem Cytochem. 2024;57:35-46 pubmed publisher
Sauvé F, Nampoothiri S, Clarke S, Fernandois D, Ferreira Co xea lho C, Dewisme J, et al. Long-COVID cognitive impairments and reproductive hormone deficits in men may stem from GnRH neuronal death. EBioMedicine. 2023;96:104784 pubmed publisher
Altounian M, Bellon A, Mann F. Neuronal miR-17-5p contributes to interhemispheric cortical connectivity defects induced by prenatal alcohol exposure. Cell Rep. 2023;42:113020 pubmed publisher
Suzuki K, Kunisada Y, Miyamura N, Eikawa S, Hurtado de Mendoza T, Mose E, et al. Tumor-resident regulatory T cells in pancreatic cancer express the αvβ5 integrin as a targetable activation marker. bioRxiv. 2023;: pubmed publisher
Bosseboeuf E, Chikh A, Chaker A, Mitchell T, Vignaraja D, Rajendrakumar R, et al. Neuropilin-1 interacts with VE-cadherin and TGFBR2 to stabilize adherens junctions and prevent activation of endothelium under flow. Sci Signal. 2023;16:eabo4863 pubmed publisher
Matsuda T, Misato K, Tamiya S, Akeda Y, Nakase I, Kuroda E, et al. Efficient antigen delivery by dendritic cell-targeting peptide via nucleolin confers superior vaccine effects in mice. iScience. 2022;25:105324 pubmed publisher
Martins L, Brambilla I, Motta A, de Pretis S, Bhat G, Badaloni A, et al. Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity. Neuron. 2022;110:4090-4107.e11 pubmed publisher
Rossignol J, Belaid Z, Fouquet G, Guillem F, Rignault R, Milpied P, et al. Neuropilin-1 cooperates with PD-1 in CD8+ T cells predicting outcomes in melanoma patients treated with anti-PD1. iScience. 2022;25:104353 pubmed publisher
Chen M, Pekosz A, Villano J, Shen W, Zhou R, Kulaga H, et al. Evolution of nasal and olfactory infection characteristics of SARS-CoV-2 variants. bioRxiv. 2022;: pubmed publisher
Boulan B, Ravanello C, Peyrel A, Bosc C, Delphin C, Appaix F, et al. CRMP4-mediated fornix development involves Semaphorin-3E signaling pathway. elife. 2021;10: pubmed publisher
Termini C, Pang A, Fang T, Roos M, Chang V, Zhang Y, et al. Neuropilin 1 regulates bone marrow vascular regeneration and hematopoietic reconstitution. Nat Commun. 2021;12:6990 pubmed publisher
Galindo Riera N, Newbold S, Sledziowska M, Llinares Benadero C, Griffiths J, Mire E, et al. Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment. Eneuro. 2021;8: pubmed publisher
Dani N, Herbst R, McCabe C, Green G, Kaiser K, Head J, et al. A cellular and spatial map of the choroid plexus across brain ventricles and ages. Cell. 2021;184:3056-3074.e21 pubmed publisher
Andriessen E, Binet F, Fournier F, Hata M, Dejda A, Mawambo G, et al. Myeloid-resident neuropilin-1 promotes choroidal neovascularization while mitigating inflammation. EMBO Mol Med. 2021;13:e11754 pubmed publisher
Limoni G, Murthy S, Jabaudon D, Dayer A, Niquille M. PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination. Cell Rep. 2021;34:108644 pubmed publisher
Abdullah A, Akhand S, Paez J, Brown W, Pan L, Libring S, et al. Epigenetic targeting of neuropilin-1 prevents bypass signaling in drug-resistant breast cancer. Oncogene. 2021;40:322-333 pubmed publisher
Apóstolo N, Smukowski S, Vanderlinden J, Condomitti G, Rybakin V, Ten Bos J, et al. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer. Nat Commun. 2020;11:5171 pubmed publisher
Gonzales J, Le Berre Scoul C, Dariel A, Bréhéret P, Neunlist M, Boudin H. Semaphorin 3A controls enteric neuron connectivity and is inversely associated with synapsin 1 expression in Hirschsprung disease. Sci Rep. 2020;10:15119 pubmed publisher
Danelon V, Goldner R, Martinez E, Gokhman I, Wang K, Yaron A, et al. Modular and Distinct Plexin-A4/FARP2/Rac1 Signaling Controls Dendrite Morphogenesis. J Neurosci. 2020;40:5413-5430 pubmed publisher
Pemmari T, Ivanova L, May U, Lingasamy P, Tobi A, Pasternack A, et al. Exposed CendR Domain in Homing Peptide Yields Skin-Targeted Therapeutic in Epidermolysis Bullosa. Mol Ther. 2020;28:1833-1845 pubmed publisher
Morin E, Lindskog C, Johansson M, Egevad L, Sandstrom P, Harmenberg U, et al. Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma. J Pathol. 2020;250:387-396 pubmed publisher
Luck R, Urban S, Karakatsani A, Harde E, Sambandan S, Nicholson L, et al. VEGF/VEGFR2 signaling regulates hippocampal axon branching during development. elife. 2019;8: pubmed publisher
Barry D, McMillan E, Kunar B, Lis R, Zhang T, Lu T, et al. Molecular determinants of nephron vascular specialization in the kidney. Nat Commun. 2019;10:5705 pubmed publisher
Taroc E, Naik A, Lin J, Peterson N, Keefe D, Genis E, et al. Gli3 Regulates Vomeronasal Neurogenesis, Olfactory Ensheathing Cell Formation, and GnRH-1 Neuronal Migration. J Neurosci. 2020;40:311-326 pubmed publisher
Klibi J, Li S, Amable L, Joseph C, Brunet S, Delord M, et al. Characterization of the developmental landscape of murine RORγt+ iNKT cells. Int Immunol. 2020;32:105-116 pubmed publisher
Hossain M, Tsuzuki T, Sakakibara K, Imaizumi F, Ikegaya A, Inagaki M, et al. PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice. PLoS ONE. 2019;14:e0221440 pubmed publisher
Bye C, Rytova V, Alsanie W, Parish C, Thompson L. Axonal Growth of Midbrain Dopamine Neurons is Modulated by the Cell Adhesion Molecule ALCAM Through Trans-Heterophilic Interactions with L1cam, Chl1, and Semaphorins. J Neurosci. 2019;39:6656-6667 pubmed publisher
Campos Mora M, Contreras Kallens P, Gálvez Jirón F, Rojas M, Rojas C, Refisch A, et al. CD4+Foxp3+T Regulatory Cells Promote Transplantation Tolerance by Modulating Effector CD4+ T Cells in a Neuropilin-1-Dependent Manner. Front Immunol. 2019;10:882 pubmed publisher
Nilsson D, Heglind M, Arani Z, Enerback S. Foxc2 is essential for podocyte function. Physiol Rep. 2019;7:e14083 pubmed publisher
LEE P, Gao N, Dike M, Shkilnyy O, Me R, Zhang Y, et al. Opposing Effects of Neuropilin-1 and -2 on Sensory Nerve Regeneration in Wounded Corneas: Role of Sema3C in Ameliorating Diabetic Neurotrophic Keratopathy. Diabetes. 2019;68:807-818 pubmed publisher
Wilson A, Shao Z, Grenier V, Mawambo G, Daudelin J, Dejda A, et al. Neuropilin-1 expression in adipose tissue macrophages protects against obesity and metabolic syndrome. Sci Immunol. 2018;3: pubmed publisher
Lee H, Macpherson L, PARADA C, Zuker C, Ryba N. Rewiring the taste system. Nature. 2017;548:330-333 pubmed publisher
Inokuchi K, Imamura F, Takeuchi H, Kim R, Okuno H, Nishizumi H, et al. Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses. Nat Commun. 2017;8:15977 pubmed publisher
Pinskey J, Franks N, McMellen A, Giger R, Allen B. Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif. J Biol Chem. 2017;292:15192-15204 pubmed publisher
Yang N, Zhang W, He T, Xing Y. Suppression of Retinal Neovascularization by Inhibition of Galectin-1 in a Murine Model of Oxygen-Induced Retinopathy. J Ophthalmol. 2017;2017:5053035 pubmed publisher
Yang N, Zhang W, He T, Xing Y. Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy. Exp Eye Res. 2017;159:1-15 pubmed publisher
Fernández Robredo P, Selvam S, Powner M, Sim D, Fruttiger M. Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation. PLoS ONE. 2017;12:e0169865 pubmed publisher
Zapiec B, Bressel O, Khan M, Walz A, Mombaerts P. Neuropilin-1 and the Positions of Glomeruli in the Mouse Olfactory Bulb. Eneuro. 2016;3: pubmed
Saito Y, Miranda Rottmann S, Ruggiu M, Park C, Fak J, Zhong R, et al. NOVA2-mediated RNA regulation is required for axonal pathfinding during development. elife. 2016;5: pubmed publisher
product information
brand :
R&D Systems
master code :
AF566
SKU :
AF566
product name :
Mouse/Rat Neuropilin-1 Antibody
unit size :
100 ug (also 25 ug)
seo description :
The Mouse/Rat Neuropilin-1 Antibody from R&D Systems is a goat polyclonal antibody to Neuropilin-1. This antibody reacts with avian - chicken,human,mouse,rat,transgenic mouse. The Mouse/Rat Neuropilin-1 Antibody has been validated for the following applications: Western Blot,Flow Cytometry,Immunohistochemistry,Immunocytochemistry,Neutralization,Immunoprecipitation,Functional Assay,Cell Culture,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Blockade of Receptor-ligand Interaction,CyTOF-ready.
target :
Neuropilin-1
category :
Primary Antibodies
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.
clonality :
Polyclonal
concentration :
LYOPH
conjugate :
Unconjugated
dilution :
Western Blot 0.1 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 3-15 ug/mL, Blockade of Receptor-ligand Interaction, CyTOF-ready
host :
Goat
immunogen :
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin-1, Phe22-Ala810 (Lys811Arg), Ser829-Asp854, Accession # Q9QWJ9
isotype :
IgG
purity :
Antigen Affinity-purified
species :
Avian - Chicken,Human,Mouse,Rat,Transgenic Mouse
specificity :
Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
gene symbol :
NRP1
endotoxin note :
<0.10 EU per 1 ╡g of the antibody by the LAL method.
Antibody validation :
Knockout/Knockdown
top caption :
Detection of Recombinant Human, Mouse, and Rat Neuropilin-1 antibody by Western Blot.
accessionNumbers :
Q9QWJ9
applications :
Blockade of Receptor-ligand Interaction,CyTOF-ready,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry,Functional Assay,Cell Culture
USD :
509 USD
alt names :
BDCA4, BDCA-4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, neuropilin 1, neuropilin-1, Neuropilin1, NP1, NRP, NRP1, transmembrane receptor, Vascular endothelial cell growth factor 165 receptor, VEGF165R
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.