Rat GFR alpha-1/GDNF R alpha-1 Antibody | R&D Systems AF560 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Rat GFR alpha-1/GDNF R alpha-1 Antibody

catalog :

AF560

quantity :

100 ug (also 25 ug)

price :

499 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig 5b	Dong F, Chen M, Jiang L, Shen Z, Ma L, Han C, et al. PRMT5 Is Involved in Spermatogonial Stem Cells Maintenance by Regulating Plzf Expression via Modulation of Lysine Histone Modifications. Front Cell Dev Biol. 2021;9:673258 pubmed publisher
	Nitahara K, Kawamura A, Kitamura Y, Kato K, Namekawa S, Nishiyama M. Chromatin remodeler CHD8 is required for spermatogonial proliferation and early meiotic progression. Nucleic Acids Res. 2024;52:2995-3010 pubmed publisher
	Anbarci D, McKey J, Levic D, Bagnat M, Capel B. Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary. bioRxiv. 2024;: pubmed publisher
	Whiley P, Nathaniel B, Stanton P, Hobbs R, Loveland K. Spermatogonial fate in mice with increased activin A bioactivity and testicular somatic cell tumours. Front Cell Dev Biol. 2023;11:1237273 pubmed publisher
	Luo Y, Yamada M, N Tumba Byn T, Asif H, Gao M, Hu Y, et al. SPRY4-dependent ERK negative feedback demarcates functional adult stem cells in the male mouse germline†. Biol Reprod. 2023;109:533-551 pubmed publisher
	Feng X, Matsumura T, Yamashita Y, Sato T, Hashimoto K, Odaka H, et al. In vitro spermatogenesis in isolated seminiferous tubules of immature mice. PLoS ONE. 2023;18:e0283773 pubmed publisher
	Peng Y, Tang X, Shu H, Dong W, Shao H, Zhou B. Sertoli cells are the source of stem cell factor for spermatogenesis. Development. 2023;150: pubmed publisher
	Nieto Rostro M, Patel R, Dickenson A, Dolphin A. Nerve injury increases native Ca V 2.2 trafficking in dorsal root ganglion mechanoreceptors. Pain. 2023;164:1264-1279 pubmed publisher
	Blount Q, Hernandez Morato I, Moayedi Y, Pitman M. Expression of Glial Cell-Derived Neurotrophic Factor Receptors Within Nucleus Ambiguus During Rat Development. Laryngoscope. 2023;133:2240-2247 pubmed publisher
	Prakash Yadav R, Leskinen S, Ma L, M xe4 kel xe4 J, Kotaja N. Chromatin remodelers HELLS, WDHD1 and BAZ1A are dynamically expressed during mouse spermatogenesis. Reproduction. 2023;165:49-63 pubmed publisher
	Uchida A, Imaimatsu K, Suzuki H, Han X, Ushioda H, Uemura M, et al. SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse. Nat Commun. 2022;13:7860 pubmed publisher
	Khan M, Cherukuri P, Negro F, Rajput A, Fabrowski P, Bansal V, et al. ERR2 and ERR3 promote the development of gamma motor neuron functional properties required for proprioceptive movement control. PLoS Biol. 2022;20:e3001923 pubmed publisher
	Kirsanov O, Johnson T, Malachowski T, Niedenberger B, Gilbert E, Bhowmick D, et al. Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro. Development. 2022;149: pubmed publisher
	Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro K, et al. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis. Commun Biol. 2022;5:504 pubmed publisher
	Barroca V, Racine C, Pays L, Fouchet P, Coureuil M, Allemand I. The netrin-1 receptor UNC5C contributes to the homeostasis of undifferentiated spermatogonia in adult mice. Stem Cell Res. 2022;60:102723 pubmed publisher
	Zou Q, Yang L, Shi R, Qi Y, Zhang X, Qi H. Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis. iScience. 2021;24:103396 pubmed publisher
	Muzyka V, Badea T. Genetic interplay between transcription factor Pou4f1/Brn3a and neurotrophin receptor Ret in retinal ganglion cell type specification. Neural Dev. 2021;16:5 pubmed publisher
	Mori Y, Takashima S, Kanatsu Shinohara M, Yi Z, Shinohara T. Cdc42 is required for male germline niche development in mice. Cell Rep. 2021;36:109550 pubmed publisher
	Yamamuro T, Nakamura S, Yamano Y, Endo T, Yanagawa K, Tokumura A, et al. Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function. PLoS Genet. 2021;17:e1009688 pubmed publisher
	Osawa Y, Murata K, Usui M, Kuba Y, Le H, Mikami N, et al. EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. elife. 2021;10: pubmed publisher
	Suzuki S, McCarrey J, Hermann B. Differential RA responsiveness among subsets of mouse late progenitor spermatogonia. Reproduction. 2021;161:645-655 pubmed publisher
	Imura Kishi K, Uchida A, Tsunekawa N, Suzuki H, Takase H, Hirate Y, et al. Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules. Sci Rep. 2021;11:1110 pubmed publisher
	Tsuchimoto A, Tone M, Ogonuki N, Hada M, Ogura A, Takashima S. Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency. Sci Rep. 2020;10:10796 pubmed publisher
	Bonafina A, Trinchero M, Ríos A, Bekinschtein P, SCHINDER A, Paratcha G, et al. GDNF and GFRα1 Are Required for Proper Integration of Adult-Born Hippocampal Neurons. Cell Rep. 2019;29:4308-4319.e4 pubmed publisher
	Varuzhanyan G, Rojansky R, Sweredoski M, Graham R, Hess S, Ladinsky M, et al. Mitochondrial fusion is required for spermatogonial differentiation and meiosis. elife. 2019;8: pubmed publisher
	Sharma M, Srivastava A, Fairfield H, BERGSTROM D, Flynn W, Braun R. Identification of EOMES-expressing spermatogonial stem cells and their regulation by PLZF. elife. 2019;8: pubmed publisher
	Cao J, Lin Z, Tong M, Zhang Y, Li Y, Zhou Y. Mechanistic target of rapamycin kinase (Mtor) is required for spermatogonial proliferation and differentiation in mice. Asian J Androl. 2020;22:169-176 pubmed publisher
	Legrand J, Chan A, La H, Rossello F, Ankö M, Fuller Pace F, et al. DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia. Nat Commun. 2019;10:2278 pubmed publisher
	Tang R, Fan L. PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation. Asian J Androl. 2019;21:309-318 pubmed publisher
	Hermann B, Cheng K, Singh A, Roa De La Cruz L, Mutoji K, Chen I, et al. The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids. Cell Rep. 2018;25:1650-1667.e8 pubmed publisher
	Guo J, Grow E, Mlcochova H, Maher G, Lindskog C, Nie X, et al. The adult human testis transcriptional cell atlas. Cell Res. 2018;28:1141-1157 pubmed publisher
	Serra N, Velte E, Niedenberger B, Kirsanov O, Geyer C. The mTORC1 component RPTOR is required for maintenance of the foundational spermatogonial stem cell pool in mice†. Biol Reprod. 2019;100:429-439 pubmed publisher
	Niedenberger B, Geyer C. Advanced immunostaining approaches to study early male germ cell development. Stem Cell Res. 2018;27:162-168 pubmed publisher
	Kurita Suzuki A, Kamo Y, Uchida C, Tanemura K, Hara K, Uchida T. Prolyl isomerase Pin1 is required sperm production by promoting mitosis progression of spermatogonial stem cells. Biochem Biophys Res Commun. 2018;497:388-393 pubmed publisher
	Arnold J, Cantu M, Kasanga E, Nejtek V, Papa E, Bugnariu N, et al. Aging-related limit of exercise efficacy on motor decline. PLoS ONE. 2017;12:e0188538 pubmed publisher
	Schaller S, Buttigieg D, Alory A, Jacquier A, Barad M, Merchant M, et al. Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons. Proc Natl Acad Sci U S A. 2017;114:E2486-E2493 pubmed publisher

product information

brand :

R&D Systems

master code :

AF560

SKU :

AF560

product name :

Rat GFR alpha-1/GDNF R alpha-1 Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Rat GFR alpha-1/GDNF R alpha-1 Antibody from R&D Systems is a goat polyclonal antibody to GFR alpha-1/GDNF R alpha-1. This antibody reacts with avian - chicken,human,mouse,porcine,rat,transgenic mouse. The Rat GFR alpha-1/GDNF R alpha-1 Antibody has been validated for the following applications: Immunohistochemistry,IFC,Immunocytochemistry,Immunochromatography,Flow Cytometry,Western Blot,Neutralization,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Blockade of Receptor-ligand Interaction.

target :

GFR alpha-1/GDNF R alpha-1

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 0.2 ug/mL, Immunohistochemistry 5-15 ug/mL, Blockade of Receptor-ligand Interaction

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant rat GFR alpha -1/GDNF R alpha -1, Asp25-Leu445, Accession # Q62997

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Avian - Chicken,Human,Mouse,Porcine,Rat,Transgenic Mouse

specificity :

Detects rat GFR alpha -1/GDNF R alpha -1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha -1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha -2 is observed.

gene symbol :

GFRA1

top caption :

Detection of Rat GFRa-1/GDNF Ra-1 antibody by Western Blot.

accessionNumbers :

Q62997

applications :

Blockade of Receptor-ligand Interaction,Immunochromatography,IFC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry

USD :

499 USD

alt names :

DKFZp313E1012, DKFZp686J0156, GDNF family receptor alpha 1, GDNF family receptor alpha-1, GDNF R alpha-1, GDNF receptor alpha-1, GDNFRAGFR-ALPHA-1, GDNFR-alpha-1, GDNFRMGC23045, GFR alpha1, GFRa-1, GFRA1, GFR-alpha-1, Glial cell line-derived neurotrophic factor receptor alpha, GPI-linked anchor protein, PI-linked cell-surface accessory protein, RET ligand 1, RET1L, RETL1FLJ10561, TGF-beta related neurotrophic factor receptor 1, TGF-beta-related neurotrophic factor receptor 1, TRNR1FLJ31546

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage