Mouse Leptin R Antibody | R&D Systems AF497 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Mouse Leptin R Antibody

catalog :

AF497

quantity :

100 ug (also 25 ug)

price :

499 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 32

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig 8a	Berger C, Heyne H, Heiland T, Dommel S, Höfling C, Guiu Jurado E, et al. A novel compound heterozygous leptin receptor mutation causes more severe obesity than in Leprdb/db mice. J Lipid Res. 2021;62:100105 pubmed publisher
	Meng S, Wang Z, Liu X, Shen K, Gu Y, Yu B, et al. Uptake of ox-LDL by binding to LRP6 mediates oxidative stress-induced BMSCs senescence promoting obesity-related bone loss. Cell Signal. 2024;117:111114 pubmed publisher
	Pourebrahim R, Heinz Montoya R, Alaniz Z, Ostermann L, Lin P, Liu B, et al. Mdm2/p53 levels in bone marrow mesenchymal stromal cells are essential for maintaining the hematopoietic niche in response to DNA damage. Cell Death Dis. 2023;14:371 pubmed publisher
	Pourebrahim R, Montoya R, Alaniz Z, Ostermann L, Lin P, Liu B, et al. Mdm2/p53 levels in bone marrow mesenchymal stromal cells is essential for maintaining the hematopoietic niche in response to DNA damage. Res Sq. 2023;: pubmed publisher
	Decourtye Espiard L, Clemessy M, Leneuve P, Mire E, Ledent T, Le Bouc Y, et al. Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice. Nutrients. 2023;15: pubmed publisher
	Doolittle M, Saul D, Kaur J, Rowsey J, Vos S, Pavelko K, et al. Multiparametric senescent cell phenotyping reveals CD24 osteolineage cells as targets of senolytic therapy in the aged murine skeleton. bioRxiv. 2023;: pubmed publisher
	Mitchell C, Verovskaya E, Calero Nieto F, Olson O, Swann J, Wang X, et al. Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageing. Nat Cell Biol. 2023;25:30-41 pubmed publisher
	Zhang W, Xu Z, Hao X, He T, Li J, Shen Y, et al. Bone Metastasis Initiation Is Coupled with Bone Remodeling through Osteogenic Differentiation of NG2+ Cells. Cancer Discov. 2023;13:474-495 pubmed publisher
	Yuan S, Stewart K, Yang Y, Abdusselamoglu M, Parigi S, Feinberg T, et al. Ras drives malignancy through stem cell crosstalk with the microenvironment. Nature. 2022;612:555-563 pubmed publisher
	Jeffery E, Mann T, Pool J, Zhao Z, Morrison S. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair. Cell Stem Cell. 2022;29:1547-1561.e6 pubmed publisher
	Palmisano B, Labella R, Donsante S, Remoli C, Spica E, Coletta I, et al. GsαR201C and estrogen reveal different subsets of bone marrow adiponectin expressing osteogenic cells. Bone Res. 2022;10:50 pubmed publisher
	Sa xe7 ma M, Matteini F, Mulaw M, Hageb A, Bogeska R, Sakk V, et al. Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues. STAR Protoc. 2022;3:101483 pubmed publisher
	Long J, Leinroth A, Liao Y, Ren Y, Mirando A, Nguyen T, et al. Hypertrophic chondrocytes serve as a reservoir for marrow-associated skeletal stem and progenitor cells, osteoblasts, and adipocytes during skeletal development. elife. 2022;11: pubmed publisher
	Riffault M, Johnson G, Owen M, Javaheri B, Pitsillides A, Hoey D. Loss of Adenylyl Cyclase 6 in Leptin Receptor-Expressing Stromal Cells Attenuates Loading-Induced Endosteal Bone Formation. JBMR Plus. 2020;4:e10408 pubmed publisher
	Lee C, Song D, Park C, Choi J, Kang G, Shin S, et al. Primary cilia mediate early life programming of adiposity through lysosomal regulation in the developing mouse hypothalamus. Nat Commun. 2020;11:5772 pubmed publisher
	Marsh B, Blelloch R. Single nuclei RNA-seq of mouse placental labyrinth development. elife. 2020;9: pubmed publisher
	Zhai L, Zhao J, Bai Y, Wei W, Sun Q, Jia L. Combined effects of obesity and di-(2-ethylhexyl) phthalate on testosterone levels and kisspeptin/GPR54 expression in hypothalamus and testes of male mice. J Chin Med Assoc. 2020;83:1020-1028 pubmed publisher
	Zhong L, Yao L, Tower R, Wei Y, Miao Z, Park J, et al. Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment. elife. 2020;9: pubmed publisher
	Sacma M, Pospiech J, Bogeska R, de Back W, Mallm J, Sakk V, et al. Haematopoietic stem cells in perisinusoidal niches are protected from ageing. Nat Cell Biol. 2019;21:1309-1320 pubmed publisher
	Himburg H, Roos M, Fang T, Zhang Y, Termini C, Schlussel L, et al. Chronic myeloid leukemia stem cells require cell-autonomous pleiotrophin signaling. J Clin Invest. 2020;130:315-328 pubmed publisher
	Severe N, Karabacak N, Gustafsson K, Baryawno N, Courties G, Kfoury Y, et al. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping. Cell Stem Cell. 2019;25:570-583.e7 pubmed publisher
	Nent E, Nozaki C, Schmöle A, Otte D, Zimmer A. CB2 receptor deletion on myeloid cells enhanced mechanical allodynia in a mouse model of neuropathic pain. Sci Rep. 2019;9:7468 pubmed publisher
	Piattini F, Le Foll C, Kisielow J, Rosenwald E, Nielsen P, Lutz T, et al. A spontaneous leptin receptor point mutation causes obesity and differentially affects leptin signaling in hypothalamic nuclei resulting in metabolic dysfunctions distinct from db/db mice. Mol Metab. 2019;25:131-141 pubmed publisher
	Frisch B, Hoffman C, Latchney S, Lamere M, Myers J, Ashton J, et al. Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B. JCI Insight. 2019;5: pubmed publisher
	Shen B, Vardy K, Hughes P, Tasdogan A, Zhao Z, Yue R, et al. Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass. elife. 2019;8: pubmed publisher
	Nozaki C, Nent E, Bilkei Gorzo A, Zimmer A. Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain. Sci Rep. 2018;8:10827 pubmed publisher
	Wang X, Xie L, Crane J, Zhen G, Li F, Yang P, et al. Aberrant TGF-β activation in bone tendon insertion induces enthesopathy-like disease. J Clin Invest. 2018;128:846-860 pubmed publisher
	Coutu D, Kokkaliaris K, Kunz L, Schroeder T. Multicolor quantitative confocal imaging cytometry. Nat Methods. 2018;15:39-46 pubmed publisher
	Li C, Chai Y, Wang L, Gao B, Chen H, Gao P, et al. Programmed cell senescence in skeleton during late puberty. Nat Commun. 2017;8:1312 pubmed publisher
	Chang K, Lin Y, Lin C, Tsai M, Huang W, Shih Y, et al. Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion. Data Brief. 2017;15:567-572 pubmed publisher
	Martin R, Cordova C, Gutierrez B, Hernández M, Nieto M. A dangerous liaison: Leptin and sPLA2-IIA join forces to induce proliferation and migration of astrocytoma cells. PLoS ONE. 2017;12:e0170675 pubmed publisher
	Asada N, Kunisaki Y, Pierce H, Wang Z, Fernandez N, Birbrair A, et al. Differential cytokine contributions of perivascular haematopoietic stem cell niches. Nat Cell Biol. 2017;19:214-223 pubmed publisher

product information

brand :

R&D Systems

master code :

AF497

SKU :

AF497

product name :

Mouse Leptin R Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Mouse Leptin R Antibody from R&D Systems is a goat polyclonal antibody to Leptin R. This antibody reacts with human,mouse,transgenic mouse. The Mouse Leptin R Antibody has been validated for the following applications: Immunohistochemistry,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-ready.

target :

Leptin R

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 0.1 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, CyTOF-ready

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant mouse Leptin R, Ala20-Gly839, Accession # Q3US58

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Transgenic Mouse

specificity :

Detects mouse Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Leptin R is observed.

gene symbol :

LEPR

accessionNumbers :

Q3US58

applications :

CyTOF-ready,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Immunocytochemistry

USD :

499 USD

alt names :

B219, CD295, CD295 antigen, DB, DKFZp686B1731, huB219, LEPR, LEP-R, leptin receptor, LeptinR, OB R, OB receptor, OB-R, OBRCD295

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage