Human/Mouse/Rat Ephrin-B2 Antibody | R&D Systems AF496 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human/Mouse/Rat Ephrin-B2 Antibody

catalog :

AF496

quantity :

100 ug (also 25 ug)

price :

509 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, proximity ligation assay

more info or order :

R&D Systems product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; loading ...; fig 1e	Fan Z, Turiel G, Ardicoglu R, Ghobrial M, Masschelein E, Kocijan T, et al. Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4+ endothelial cells. Cell Metab. 2021;: pubmed publisher
	Banerjee K, Lin Y, Gahn J, Cordero J, Gupta P, Mohamed I, et al. SMAD4 maintains the fluid shear stress set point to protect against arterial-venous malformations. J Clin Invest. 2023;133: pubmed publisher
	Urban M, Charsar B, Heinsinger N, Markandaiah S, Sprimont L, Zhou W, et al. EphrinB2 knockdown in spinal cord astrocytes preserves diaphragm innervation in a mutant SOD1 mouse model of ALS. bioRxiv. 2023;: pubmed publisher
	Takaya K, Okabe K, Ishigami A, Imbe Y, Kanazawa H, Sakai S, et al. Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration. Sci Rep. 2022;12:15913 pubmed publisher
	Ang L, Nguyen A, Liu K, Chen A, Xiong X, Curtis M, et al. Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra viruses. Cell. 2022;185:2523-2541.e30 pubmed publisher
	Maruoka H, Zhao S, Yoshino H, Abe M, Yamamoto T, Hongo H, et al. Histochemical examination of blood vessels in murine femora with intermittent PTH administration. J Oral Biosci. 2022;64:329-336 pubmed publisher
	Guaiquil V, Xiao C, Lara D, Dimailig G, Zhou Q. Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury. Exp Eye Res. 2022;219:109054 pubmed publisher
	Yamasaki S, Kuwahara A, Kishino A, Kimura T, Takahashi M, Mandai M. Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture. Regen Ther. 2022;19:24-34 pubmed publisher
	Wang Y, Shao C, Qi L, Tan J, Zhao Y, Xue M, et al. EphrinB2-RhoA upregulation attenuates sympathetic hyperinnervation and decreases the incidence of ventricular arrhythmia after myocardial infarction. J Cardiol. 2022;79:423-431 pubmed publisher
	Ebrahim A, Hailat Z, Bandyopadhyay S, Neill D, Kandouz M. The Value of EphB2 Receptor and Cognate Ephrin Ligands in Prognostic and Predictive Assessments of Human Breast Cancer. Int J Mol Sci. 2021;22: pubmed publisher
	Wang T, Liu J, Liu H, Lee S, Gonzalez L, Gorecka J, et al. Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous Fistulae. J Surg Res. 2021;262:224-239 pubmed publisher
	Frye M, Stritt S, Ortsäter H, Hernandez Vasquez M, Kaakinen M, Vicente A, et al. EphrinB2-EphB4 signalling provides Rho-mediated homeostatic control of lymphatic endothelial cell junction integrity. elife. 2020;9: pubmed publisher
	Ge Y, Feng K, Liu X, Chen H, Sun Z, Wang C, et al. The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo. Oxid Med Cell Longev. 2020;2020:1404915 pubmed publisher
	Shimizu Y, Nakamura K, Yoshii A, Yokoi Y, Kikuchi M, Shinozaki R, et al. Paneth cell α-defensin misfolding correlates with dysbiosis and ileitis in Crohn's disease model mice. Life Sci Alliance. 2020;3: pubmed publisher
	Li M, Nishio S, Naruse C, Riddell M, Sapski S, Katsuno T, et al. Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome. Nat Commun. 2020;11:1343 pubmed publisher
	Harde E, Nicholson L, Furones Cuadrado B, Bissen D, Wigge S, Urban S, et al. EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development. elife. 2019;8: pubmed publisher
	Altalhi W, Hatkar R, Hoying J, Aghazadeh Y, Nunes S. Type I Diabetes Delays Perfusion and Engraftment of 3D Constructs by Impinging on Angiogenesis; Which can be Rescued by Hepatocyte Growth Factor Supplementation. Cell Mol Bioeng. 2019;12:443-454 pubmed publisher
	Shi W, Wang Y, Peng J, Qi S, Vitale N, Kaneda N, et al. EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells. J Biol Chem. 2019;294:6871-6887 pubmed publisher
	Coucha M, Barrett A, Elgebaly M, Ergul A, Abdelsaid M. Inhibition of Ephrin-B2 in brain pericytes decreases cerebral pathological neovascularization in diabetic rats. PLoS ONE. 2019;14:e0210523 pubmed publisher
	Groppa E, Brkic S, Uccelli A, Wirth G, Korpisalo Pirinen P, Filippova M, et al. EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF. EMBO Rep. 2018;19: pubmed publisher
	Tsang K, Hyun J, Cheng K, Vargas M, Mehta D, Ushio Fukai M, et al. Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1?. Stem Cell Reports. 2017;9:796-806 pubmed publisher
	Toray H, Hasegawa T, Sakagami N, Tsuchiya E, Kudo A, Zhao S, et al. Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice. Biomed Res. 2017;38:123-134 pubmed publisher
	Depner C, Zum Buttel H, Bogurcu N, Cuesta A, Aburto M, Seidel S, et al. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance. Nat Commun. 2016;7:12329 pubmed publisher

product information

brand :

R&D Systems

master code :

AF496

SKU :

AF496

product name :

Human/Mouse/Rat Ephrin-B2 Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human/Mouse/Rat Ephrin-B2 Antibody from R&D Systems is a goat polyclonal antibody to Ephrin-B2. This antibody reacts with human,mouse,rat,transgenic mouse,frog - xenopus (african clawed frog). The Human/Mouse/Rat Ephrin-B2 Antibody has been validated for the following applications: Immunofluorescence,Immunocytochemistry,Flow Cytometry,Immunohistochemistry,Proximity Ligation Assay (PLA),Immunoprecipitation,Western Blot,Functional Assay,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,CyTOF-ready.

target :

Ephrin-B2

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunocytochemistry 5-15 ug/mL, CyTOF-ready

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-B2, Arg27-Ala227, Accession # AAA82934

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Rat,Transgenic Mouse,Frog - Xenopus (African Clawed Frog)

specificity :

Detects mouse Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Ephrin-B1 is observed.

gene symbol :

EFNB2

top caption :

Detection of Mouse Ephrin-B2 antibody by Western Blot.

accessionNumbers :

AAA82934

applications :

CyTOF-ready,Proximity Ligation Assay (PLA),Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,Functional Assay

USD :

509 USD

alt names :

EFNB2, ELF-2, ephrin B2, EphrinB2, EPLG5, Htk-L, HTKL, LERK5, LERK-5, NLERK-1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage