Human/Mouse EphB2 Antibody | R&D Systems AF467 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Human/Mouse EphB2 Antibody

catalog :

AF467

quantity :

100 ug (also 25 ug)

price :

509 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation

more info or order :

R&D Systems product webpage

citations: 34

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; loading ...; fig 1e	Huang Z, Liu S, Tang A, Al Rabadi L, Henkemeyer M, Mimche P, et al. Key role for EphB2 receptor in kidney fibrosis. Clin Sci (Lond). 2021;135:2127-2142 pubmed publisher
	Weible Ii M, Lovelace M, Mundell H, Pang T, Chan Ling T. BMPRII+ neural precursor cells isolated and characterized from organotypic neurospheres: an in vitro model of human fetal spinal cord development. Neural Regen Res. 2024;19:447-457 pubmed publisher
	Chang C, Banerjee S, Park S, Zhang X, Cotnoir White D, Opperman K, et al. Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function. bioRxiv. 2023;: pubmed publisher
	Gonz xe1 lez Gonz xe1 lez I, Gray J, Ferreira J, Conde Dusman M, Bouchet D, Perez Ota xf1 o I, et al. GluN3A subunit tunes NMDA receptor synaptic trafficking and content during postnatal brain development. Cell Rep. 2023;42:112477 pubmed publisher
	Suzuki Y, Okabayashi K, Hasegawa H, Tsuruta M, Seishima R, Tokuda T, et al. Role of EphB2/ephrin-B1 signalling in the development and progression of obesity-associated colorectal cancer. Oncol Lett. 2022;24:316 pubmed publisher
	Azkanaz M, Corominas Murtra B, Ellenbroek S, Bruens L, Webb A, Laskaris D, et al. Retrograde movements determine effective stem cell numbers in the intestine. Nature. 2022;607:548-554 pubmed publisher
	Banerjee S, Lessard F, Chartier F, Jacquet K, Osornio Hernandez A, Teyssier V, et al. EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks. Cell Rep. 2022;40:111031 pubmed publisher
	Guaiquil V, Xiao C, Lara D, Dimailig G, Zhou Q. Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury. Exp Eye Res. 2022;219:109054 pubmed publisher
	Lewis A, Kuwahara A, Franzosi J, Bush J. Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting. Cell Rep. 2022;38:110510 pubmed publisher
	López Arribillaga E, Yan B, Lobo Jarne T, Guillén Y, Menendez S, Andreu M, et al. Accumulation of Paneth Cells in Early Colorectal Adenomas Is Associated with Beta-Catenin Signaling and Poor Patient Prognosis. Cells. 2021;10: pubmed publisher
	M Gagn xe9 L, Morin N, Lavoie N, Bisson N, Lambert J, Mallette F, et al. Tyrosine phosphorylation of DEPTOR functions as a molecular switch to activate mTOR signaling. J Biol Chem. 2021;297:101291 pubmed publisher
	Zhou J, Lin Y, Huynh T, Noguchi H, Bush J, Pleasure S. NMDA receptors control development of somatosensory callosal axonal projections. elife. 2021;10: pubmed publisher
	Ahmed M, Wang P, Nguyen N, Nakada Y, Menendez Montes I, Ismail M, et al. Identification of tetracycline combinations as EphB1 tyrosine kinase inhibitors for treatment of neuropathic pain. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
	Kischel A, Audouard C, Fawal M, Davy A. Ephrin-B2 paces neuronal production in the developing neocortex. BMC Dev Biol. 2020;20:12 pubmed publisher
	Morral C, Stanisavljevic J, Hernando Momblona X, Mereu E, Álvarez Varela A, Cortina C, et al. Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer. Cell Stem Cell. 2020;26:845-861.e12 pubmed publisher
	Wei S, Huang Y, Hsieh M, Lin Y, Shyu W, Chen H, et al. Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling. Cell Death Dis. 2020;11:307 pubmed publisher
	Vreeken D, Bruikman C, Cox S, Zhang H, Lalai R, Koudijs A, et al. EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands. J Leukoc Biol. 2020;: pubmed publisher
	Yoshida S, Miwa H, Kawachi T, Kume S, Takahashi K. Generation of intestinal organoids derived from human pluripotent stem cells for drug testing. Sci Rep. 2020;10:5989 pubmed publisher
	Farr L, Ghosh S, Jiang N, Watanabe K, Parlak M, Bucala R, et al. CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing. Cell Mol Gastroenterol Hepatol. 2020;10:101-112 pubmed publisher
	Washburn H, Xia N, Zhou W, Mao Y, Dalva M. Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility. Nat Commun. 2020;11:570 pubmed publisher
	Liu Y, Horning A, Lieberman B, Kim M, Lin C, Hung C, et al. Spatial EGFR Dynamics and Metastatic Phenotypes Modulated by Upregulated EphB2 and Src Pathways in Advanced Prostate Cancer. Cancers (Basel). 2019;11: pubmed publisher
	Zhang Z, Ye M, Li Q, You Y, Yu H, Ma Y, et al. The Schizophrenia Susceptibility Gene OPCML Regulates Spine Maturation and Cognitive Behaviors through Eph-Cofilin Signaling. Cell Rep. 2019;29:49-61.e7 pubmed publisher
	Wu Z, Ashlin T, Xu Q, Wilkinson D. Role of forward and reverse signaling in Eph receptor and ephrin mediated cell segregation. Exp Cell Res. 2019;381:57-65 pubmed publisher
	Ernst A, Böhler L, Hagenston A, Hoffmann A, Heiland S, Sticht C, et al. EphB2-dependent signaling promotes neuronal excitotoxicity and inflammation in the acute phase of ischemic stroke. Acta Neuropathol Commun. 2019;7:15 pubmed publisher
	Mimche P, Lee C, Mimche S, Thapa M, Grakoui A, Henkemeyer M, et al. EphB2 receptor tyrosine kinase promotes hepatic fibrogenesis in mice via activation of hepatic stellate cells. Sci Rep. 2018;8:2532 pubmed publisher
	Morgenstern Y, Das Adhikari U, Ayyash M, Elyada E, Tóth B, Moor A, et al. Casein kinase 1-epsilon or 1-delta required for Wnt-mediated intestinal stem cell maintenance. EMBO J. 2017;36:3046-3061 pubmed publisher
	Miura S, Suzuki A. Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming. Cell Stem Cell. 2017;21:456-471.e5 pubmed publisher
	Liu Y, Tsai J, Chen J, Yang W, Chang P, Cheng P, et al. Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon. Sci Rep. 2017;7:42895 pubmed publisher
	Tüysüz N, van Bloois L, van den Brink S, Begthel H, Verstegen M, Cruz L, et al. Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells. Nat Commun. 2017;8:14578 pubmed publisher
	Shi X, Sun K, Hu R, Liu X, Hu Q, Sun X, et al. Blocking the Interaction between EphB2 and ADDLs by a Small Peptide Rescues Impaired Synaptic Plasticity and Memory Deficits in a Mouse Model of Alzheimer's Disease. J Neurosci. 2016;36:11959-11973 pubmed
	van Lidth de Jeude J, Meijer B, Wielenga M, Spaan C, Baan B, Rosekrans S, et al. Induction of endoplasmic reticulum stress by deletion of Grp78 depletes Apc mutant intestinal epithelial stem cells. Oncogene. 2017;36:3397-3405 pubmed publisher
	O Neill A, Kindberg A, Niethamer T, Larson A, Ho H, Greenberg M, et al. Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation. J Cell Biol. 2016;215:217-229 pubmed
	Cisse M, Duplan E, Lorivel T, Dunys J, Bauer C, Meckler X, et al. The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model. Mol Psychiatry. 2017;22:1562-1575 pubmed publisher
	Depner C, Zum Buttel H, Bogurcu N, Cuesta A, Aburto M, Seidel S, et al. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance. Nat Commun. 2016;7:12329 pubmed publisher

product information

brand :

R&D Systems

master code :

AF467

SKU :

AF467

product name :

Human/Mouse EphB2 Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Human/Mouse EphB2 Antibody from R&D Systems is a goat polyclonal antibody to EphB2. This antibody reacts with human,mouse,rat,frog - xenopus (african clawed frog). The Human/Mouse EphB2 Antibody has been validated for the following applications: Western Blot,Immunohistochemistry,Immunoprecipitation,Simple Western,Neutralization,Functional Assay,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Immunofluorescence,Immunocytochemistry,Flow Cytometry,CyTOF-ready.

target :

EphB2

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 0.1 ug/mL, Simple Western 20 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 1-15 ug/mL, Immunocytochemistry 5-15 ug/mL, CyTOF-ready

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant mouse EphB2, Val27-Lys548, Accession # P54763

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Rat,Frog - Xenopus (African Clawed Frog)

specificity :

Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.

gene symbol :

EPHB2

top caption :

Detection of Recombinant Human and Mouse EphB2 antibody by Western Blot.

accessionNumbers :

P54763

applications :

Flow Cytometry,CyTOF-ready,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Simple Western,Neutralization,Immunofluorescence,Immunocytochemistry,Functional Assay

USD :

509 USD

alt names :

CAPB, Cek5, Drt, DRTEphB2, EC 2.7.10, EC 2.7.10.1, EK5, elk-related tyrosine kinase, EPH receptor B2, eph tyrosine kinase 3, EPH-like kinase 5, ephrin type-B receptor 2, EPHT3MGC87492, EPTH3, Erk, ERKHek5, HEK5, Nuk, PCBC, protein-tyrosine kinase HEK5, Qek2, Renal carcinoma antigen NY-REN-47, Sek3, Tyro5, Tyrosine-protein kinase receptor EPH-3, Tyrosine-protein kinase TYRO5

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage