Mouse EphB4 Antibody | R&D Systems AF446 product information

product summary

company name :

R&D Systems

product type :

antibody

product name :

Mouse EphB4 Antibody

catalog :

AF446

quantity :

100 ug (also 25 ug)

price :

509 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

R&D Systems product webpage

citations: 20

Reference
Chen D, Wiggins D, Sevick E, Davis M, King P. An EPHB4-RASA1 signaling complex inhibits shear stress-induced Ras-MAPK activation in lymphatic endothelial cells to promote the development of lymphatic vessel valves. bioRxiv. 2023;: pubmed publisher
Zhao S, Mekbib K, van der Ent M, Allington G, Prendergast A, Chau J, et al. Mutation of key signaling regulators of cerebrovascular development in vein of Galen malformations. Nat Commun. 2023;14:7452 pubmed publisher
Zhao S, Mekbib K, van der Ent M, Allington G, Prendergast A, Chau J, et al. Genetic dysregulation of an endothelial Ras signaling network in vein of Galen malformations. bioRxiv. 2023;: pubmed publisher
Kohara S, Ogawa K. Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells. Biomedicines. 2022;10: pubmed publisher
Maruoka H, Zhao S, Yoshino H, Abe M, Yamamoto T, Hongo H, et al. Histochemical examination of blood vessels in murine femora with intermittent PTH administration. J Oral Biosci. 2022;64:329-336 pubmed publisher
Guaiquil V, Xiao C, Lara D, Dimailig G, Zhou Q. Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury. Exp Eye Res. 2022;219:109054 pubmed publisher
Lewis A, Kuwahara A, Franzosi J, Bush J. Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting. Cell Rep. 2022;38:110510 pubmed publisher
Alam J, Ogawa K. Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary. Reprod Biol. 2021;21:100511 pubmed publisher
Donato C, Kunz L, Castro Giner F, Paasinen Sohns A, Strittmatter K, Szczerba B, et al. Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells. Cell Rep. 2020;32:108105 pubmed publisher
Luxan G, Stewen J, Díaz N, Kato K, Maney S, Aravamudhan A, et al. Endothelial EphB4 maintains vascular integrity and transport function in adult heart. elife. 2019;8: pubmed publisher
Payne S, Gunadasa Rohling M, Neal A, Redpath A, Patel J, Chouliaras K, et al. Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart. Nat Commun. 2019;10:3276 pubmed publisher
Bhatia S, Oweida A, Lennon S, Darragh L, Milner D, Phan A, et al. Inhibition of EphB4-Ephrin-B2 Signaling Reprograms the Tumor Immune Microenvironment in Head and Neck Cancers. Cancer Res. 2019;79:2722-2735 pubmed publisher
Neal A, Nornes S, Payne S, Wallace M, Fritzsche M, Louphrasitthiphol P, et al. Venous identity requires BMP signalling through ALK3. Nat Commun. 2019;10:453 pubmed publisher
Uhl C, Markel M, Broggini T, Nieminen M, Kremenetskaia I, Vajkoczy P, et al. EphB4 mediates resistance to antiangiogenic therapy in experimental glioma. Angiogenesis. 2018;21:873-881 pubmed publisher
Groppa E, Brkic S, Uccelli A, Wirth G, Korpisalo Pirinen P, Filippova M, et al. EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF. EMBO Rep. 2018;19: pubmed publisher
Toray H, Hasegawa T, Sakagami N, Tsuchiya E, Kudo A, Zhao S, et al. Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice. Biomed Res. 2017;38:123-134 pubmed publisher
Depner C, Zum Buttel H, Bogurcu N, Cuesta A, Aburto M, Seidel S, et al. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance. Nat Commun. 2016;7:12329 pubmed publisher
Fuggetta M, Bordignon V, Cottarelli A, Macchi B, Frezza C, Cordiali Fei P, et al. Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol. J Exp Clin Cancer Res. 2016;35:118 pubmed publisher
Martin Almedina S, Martínez Corral I, Holdhus R, Vicente A, Fotiou E, Lin S, et al. EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis. J Clin Invest. 2016;126:3080-8 pubmed publisher
Wang M, Collins M, Foster T, Bai H, Hashimoto T, Santana J, et al. Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase. J Vasc Surg. 2017;65:179-189 pubmed publisher

product information

brand :

R&D Systems

master code :

AF446

SKU :

AF446

product name :

Mouse EphB4 Antibody

unit size :

100 ug (also 25 ug)

seo description :

The Mouse EphB4 Antibody from R&D Systems is a goat polyclonal antibody to EphB4. This antibody reacts with human,mouse,primate - chlorocebus aethiops (african green monkey),rat,fish - danio rerio (zebrafish). The Mouse EphB4 Antibody has been validated for the following applications: Bioassay,Flow Cytometry,Immunoprecipitation,Western Blot,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,CyTOF-ready.

target :

EphB4

category :

Primary Antibodies

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 ╡m filtered solution in PBS.

clonality :

Polyclonal

concentration :

LYOPH

conjugate :

Unconjugated

dilution :

Western Blot 0.1 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, CyTOF-ready

host :

Goat

immunogen :

Mouse myeloma cell line NS0-derived recombinant mouse EphB4, Leu16-Ala539, Accession # P54761

isotype :

IgG

purity :

Antigen Affinity-purified

species :

Human,Mouse,Primate - Chlorocebus aethiops (African Green Monkey),Rat,Fish - Danio rerio (Zebrafish)

specificity :

Detects mouse EphB4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed.

gene symbol :

EPHB4

top caption :

EphB4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

accessionNumbers :

P54761

applications :

CyTOF-ready,Bioassay,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence

USD :

509 USD

alt names :

EC 2.7.10, EC 2.7.10.1, EPH receptor B4, EphB4, ephrin type-B receptor 4, hepatoma transmembrane kinase, Htk, HTKephrin receptor EphB4, Mdk2, Myk1, soluble EPHB4 variant 1, soluble EPHB4 variant 2, soluble EPHB4 variant 3, Tyro11, Tyrosine-protein kinase receptor HTK, Tyrosine-protein kinase TYRO11

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 6 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage